a******a
发帖数: 283 | 1 We want to detect a gene of interest inserted into human genome by
retrovirus, using qPCR. That said human genome will be in the background and
the gene of interest should have no overlap with human genome. However the
gene of interest is high GC content (around 70%). Currently we are building
a standard curve by mocking the reaction condition, but the result is not
that well.
Any recommendation? Anybody familiar with detecting gene insert in the
genome? Any recommendation regarding good literatures?
Thanks. | o**i
发帖数: 1165 | 2 "Currently we are building
a standard curve by mocking the reaction condition, but the result is not
that well."
can you be more specific? | a******a
发帖数: 283 | 3 Hi thank you for your reply. I have solved the question.
【在 o**i 的大作中提到】
: "Currently we are building
: a standard curve by mocking the reaction condition, but the result is not
: that well."
: can you be more specific?
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