s******e
发帖数: 111 | 1 Bio & Beyond 公司现提供免费 糖苷表面活性剂 DDM （n-dodecyl-ß-D-
maltoside）， 1克包装， 有从事膜蛋白相关的朋友可向[email protected]
/* */ 索
取, 链接是www.bioandbeyond.com, 点击最上方提示可进。 |
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X******r
发帖数: 189 | 2 换了Johnson&Johnson婴幼儿的洗发水，感觉好多了。
不喜欢里面有sodium dodecyl sulfate的东西，个人对此很敏感。 |
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T***y
发帖数: 6600 | 3 难道大家都在用“高级”的牙膏，肥皂，及洗涤剂了？这些东西里大都含有SLS。
还有，SLS致癌，基本还只是谣传：
http://zh.wikipedia.org/wiki/%E5%8D%81%E4%BA%8C%E7%83%B7%E5%9F%
十二烷基硫酸钠（Sodium dodecyl sulfate，SDS）或月桂基硫酸鈉(Sodium lauryl
sulfate SLS)，NaC12H25SO4，是洗洁精的主要成分。常用于DNA提取过程中，使蛋白质
变性后与DNA分开。通常被误读为十二烷基磺酸钠。十二烷基硫酸钠（SLS）作为发泡剂
被广泛应用于牙膏、肥皂、浴液、洗发香波、洗衣粉，以及化妆品中。95%的个人护肤
用品和家居清洁用品中都含有十二烷基硫酸钠。
此成分在一段時間當中被謠傳為致癌物，美國化妝品及衛浴、芳香劑協會(The
Cosmetic, Toiletry, and Fragrance Associaton )因此公開在其官方網站(www.ctfa.
org)上澄清，目前並無證據顯示此成分會導致癌症。美國癌症學會(American Cancer
Society)亦作如此表示。... 阅读全帖 |
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c******n
发帖数: 5697 | 4 你记错了吧? aluminum hydroxide是不会跟油其反应的, 应当是牙膏中的肥皂粉SDS,
那种dodecyl/lauryl sulfate, 可以把口腔表层的油膜洗掉 |
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T**********t
发帖数: 1604 | 5 http://dx.doi.org/10.1016/0003-2697(82)90113-0
Electrophoresis buffers for polyacrylamide gels at various pH
Tracy McLellan
Analytical Biochemistry
Volume 126, Issue 1, October 1982, Pages 94-99
http://dx.doi.org/10.1016/0003-2697(87)90587-2
Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the
separation of proteins in the range from 1 to 100 kDa
Hermann Schägger and Gebhard von Jagow
Analytical Biochemistry
Volume 166, Issue 2, 1 November 1987, Pages 368-379
http://www |
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F******y
发帖数: 1988 | 6 Anal Biochem. 1987 Nov 1;166(2):368-79.
Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the
separation of proteins in the range from 1 to 100 kDa.
Schägger H, von Jagow G.
Institut für Physikalische Biochemie der Universität München, Federal
Republic of Germany. |
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s*********y
发帖数: 387 | 7 Thanks for the infor.
Results as below from Google Scholar
the one you mentioned for methodology is 4k citation comparable to
XiaoDong's case.
"
Peptide mapping by limited proteolysis in sodium dodecyl sulfate and
analysis by gel electrophoresis.
jbc.org [PDF]… , SG Fischer, MW Kirschner, UK Laemmli - Journal of
Biological …, 1977 - ASBMB
THE JOURNAL OF Bm.oorc~~ CHEMISTRY Vol. 252, No. 3, Issue of February
10, pp.
1102-1106, 1971 Prrnfed m USA. ... Peptide Mapping by Limited
Proteolysis in Sodi |
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b*******3
发帖数: 99 | 8 To denature, use a loading buffer with the anionic denaturing detergent
sodium dodecyl sulfate (SDS), and boil the mixture at 95-100°C for 5
minutes.
Heating at 70°C for 5-10 minutes is also acceptable and may be preferable
when studying multi-pass membrane proteins. These tend to aggregate when
boiled and the aggregates may not enter the gel efficiently.
http://www.abcam.com/ps/pdf/protocols/WB-beginner.pdf
Denature |
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g***j
发帖数: 40861 | 9 s*****[email protected]
谢谢！
1.
Inactivation of Escherichia coli O157:H7 and Salmonella Typhimurium DT 104
on Alfalfa Seeds by Levulinic Acid and Sodium Dodecyl Sulfate
Authors: Zhao, Tong; Zhao, Ping; Doyle, Michael P.
Source: Journal of Food Protection®, Volume 73, Number 11, November
2010 , pp. 2010-2017(8)
2.
Control of Salmonella on Sprouting Mung Bean and Alfalfa Seeds by Using a
Biocontrol Preparation Based on Antagonistic Bacteria and Lytic
Bacteriophages
Authors: Ye, Jianxiong1; Kostrzyns... 阅读全帖 |
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l**********1
发帖数: 5204 | 10 CA630 1%
Immunoprecipitation of IMC components. Highly synchronized late-stage
parasites were purified over 70% (wt/vol) Percoll, and proteins were
extracted by
incubating them on ice for 15 min in RIPA buffer (1%
octylphenoxypolyethoxyethanol
[IGEPAL CA-630], 150 mM NaCl, 0.5% sodium deoxycholate
[DOC], 0.1% sodium dodecyl sulfate [SDS], 50 mM Tris, pH 7.4). Soluble and
insoluble fractions were separated by centrifugation at 12,000 g for 10
min at
4°C. Soluble lysates were incubated for 1 h... 阅读全帖 |
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f********3
发帖数: 8 | 11 f*********[email protected]
多谢！
High-resolution separation of peptides by sodium dodecyl sulfate-
polyacrylamide gel “focusing”
Gleb Zilberstein, Leonid Korol, Ilya Shlar, Pier Giorgio Righetti, Shmuel
Bukshpan Dr.
ELECTROPHORESIS
Volume 29, Issue 8, pages 1749–1752, No. 8 April 2008 |
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s******e
发帖数: 111 | 12 Bio & Beyond 公司现提供免费 糖苷表面活性剂 DDM （n-dodecyl-ß-D-
maltoside）， 1克包装， 有从事膜蛋白相关的朋友可向 [email protected]
/* */ 咨
询索取， 数量有限，先到先得。 该公司提供高纯度各类表面活性剂及蛋白膜分离相关
试剂， 详情请查 http://www.bioandbeyond.com/ |
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h*****b
发帖数: 492 | 13 Title: Detection of carbonylated proteins in two-dimensional sodium dodecyl
sulfate polyacrylamide gel electrophoresis separations
Journal: Methods Mol Biol. 2008;476:153-63
Authors: Sultana R, Newman SF, Huang Q, Butterfield DA.
PMID: 19157015
thanks
please send it to h*****[email protected] |
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n*****n
发帖数: 91 | 16 Journal of Chromatography A
Volume 744, Issues 1-2, 13 September 1996, Pages 295-301
Capillary isoelectric focusing and sodium dodecyl sulfate-capillary gel
electrophoresis of recombinant humanized monoclonal antibody HER2
doi:10.1016/0021-9673(96)00437-2
g******[email protected]
Thank you! |
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j**********i
发帖数: 187 | 17 Electrophoresis. 1999 Jul;20(10):2039-45.
On the mechanism of background silver staining during sodium dodecyl
sulphate-polyacrylamide gel electrophoresis.
Patras G, Qiao GG, Solomon DH.
Department of Chemical Engineering, The University of Melbourne, Parkville,
Australia. |
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M**i
发帖数: 331 | 18 Preparation of n-dodecyl mercaptan from 1-dodecanol
Huaxue Gongchengshi 2010,24(4), 62-64
w****[email protected]
Many thanks. |
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i*n
发帖数: 9793 | 19 1: Deming, S. N., J. Chromatogr. 1991, 550, 15–25.
2: Safa, F., Hadjmohammadi, M. R., J. Chromatogr. A.2005, 1078, 42–50.
3: A silver stain protocol for proteins yielding high resolution and
transparent background in sodium dodecyl sulfate-polyacrylamide gels.
Electrophoresis, 16(1995) pages 948–951.
Marianne Swain and Dr. Neil W. Ross
thanks! |
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r*****e
发帖数: 1 | 20 【 以下文字转载自 Chemistry 讨论区 】
发信人: recycle (rec), 信区: Chemistry
标 题: 请教一个英文翻译
发信站: BBS 未名空间站 (Tue Feb 27 18:43:45 2007)
Sodium dodecyl benzenesulfonate
pentafluorobenzoic acid
这两个词翻成中文咋说??
谢过好心人了~ |
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