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c********l
发帖数: 8138 | 2 屏幕分辨率低对于我这样的人是一个最大的大优点
T60p |
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b*********f
发帖数: 1585 | 3 Today’s opening keynote at NVIDIA’s GTC had a number of interesting
announcements (we’ll get to those in other articles) but the “star” of
the show was the upcoming Tesla K20.
Destined to spend its life in HPC systems and supercomputers, the K20 is
based off on a Kepler GK110 core which is billed as the “largest, fastest”
processing core ever designed. It features a threefold increase in double
precision performance over a comparable Fermi core and also incorporates
high level dynamic paralleli... 阅读全帖 |
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x*******e
发帖数: 3 | 5 I am at the bay area, founded a startup, and was in big incubator. Seen many
startups and how they work and hire.
Designers (especially UI) are in high demand right now. To get into this
path, you really don't need any certificate. What you need is to showcase
your past work (get your own blog, or put on dribbble.com/). The way to
accumulate these work is to do work for startups. You might also try
99designs.com, where you compete with other designers for work. If you love
design, these work sho... 阅读全帖 |
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x*******e
发帖数: 3 | 6 I am at the bay area, founded a startup, and was in big incubator. Seen many
startups and how they work and hire.
Designers (especially UI) are in high demand right now. To get into this
path, you really don't need any certificate. What you need is to showcase
your past work (get your own blog, or put on dribbble.com/). The way to
accumulate these work is to do work for startups. You might also try
99designs.com, where you compete with other designers for work. If you love
design, these work sho... 阅读全帖 |
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M****e
发帖数: 70 | 8 this is a good paper that tries to dissect the regulatory
mechanism of strict control on DNA replication during cell
cycle. to ensure only one round of DNA replication at each
S phase, yeast S. cerevisiae exploits cdk2 kinase to regulate
functionally reduntant pathways: degradation of Cdc6, export
of MCM from the nucleus, and phosphorylation of ORC. however,
the mechanism in metazoan is unclear while the cellular
targets of cdk2 have not been completely understood yet.
the author (only one besid |
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s*******p
发帖数: 41 | 9 Postdoctoral Fellow (Bioinformatics-focused)
This position is open immediately in the Lo Laboratory, which is focused on
melanoma research in three thematic areas:
Discovering somatically mutated genes in melanoma using exon capture and
next-generation sequencing and studying these genes in the pathogenesis of
melanoma.
Dissecting oncogene co-dependent survival networks in melanoma using V600EB-
RAF as a prototypic melanoma oncogene and siRNA library as a discovery tool.
Understand the mechanism |
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s******y
发帖数: 28562 | 10 We use Jenco (USA). It come with a digital camera adapter.
We have been using it for our mouse embryo manipulation for several years.
Pretty convenient for our work, and very very affordable. |
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a***e
发帖数: 1010 | 11 no price on their website |
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s******y
发帖数: 28562 | 12 You need to contact their sale rep for a quote. I think we got our system
for a few thousand bucks with camera, lens and other stuffs. |
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s******y
发帖数: 28562 | 13 GL7-290. We ordered it through ISC Bio-express. I think you can check the
price through their website. http://www.bioexpress.com/
E6.5 embryo? That would be very, very small. I am sure you can see it under
that microscope, but you will have to have very good hands to handle it.
We only deal with stuffs older than E12.
for |
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a***e
发帖数: 1010 | 15 nikon & olympus are so expensive |
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s******y
发帖数: 28562 | 16 I double checked the price on ISC Bioexpress, the system we are using is
only around $ 1,200. Very cheap |
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a******n
发帖数: 392 | 17 Olympus nikon leica zeiss 都生产好的解剖镜，个人觉得奥林巴斯的较好些。买之前
可以让代理提供试用。要注
意变倍范围，工作距离，照明方式（暗视野对解剖小东西很关键）。拍照的话一般不建
议用解剖镜拍6、5天的
胚胎，因为放大倍率不够。工作距离是镜头到底座的距离，直接影响解剖时的舒适度，
以大为好。 |
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n********k
发帖数: 2818 | 19 watch out Rowitch DH, last times he came here and gave a very very nice talk
. I think he is gearing up to
dissect some of those with genetic and lineage tracing tools on astrocytes,
which were very nice and
impressive... |
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m****r
发帖数: 383 | 20 嗯。顺带说一句，这个cell line长primary tumor真够快的，长出的melanoma也忒恶心
，昨天一边dissect一边想吐,nnd |
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k*****d
发帖数: 177 | 21 带友发文，请勿回站内，谢谢~
以下为全文：
I am rotating in a lab and struggling with the chick embryonic neural tube
injection. I have
already practised for at least 5 times and still could not get it. Now I can
see the neural tube
under the dissection microscope, but I feel really hard to blow and do the
injection. I think the
main problem I have is my failure to dip the micropipette in a correct way.
Or maybe I did not
blow in the correct time point, or maybe the pipette does not have a proper-
sized hole. I feel |
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g**********y
发帖数: 423 | 22 我也重复了nervins组的jeffery的subpathway dissecting的工作，太random了，结果
不能重复。 |
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n********k
发帖数: 2818 | 23 I think Qinghua Liu is doing well in the RNAi pathway, his study on kinase
is nice...and also shall watch out
Zhong qing at stanford who is dissecting authophagy with a biochemical and
purification approach...
ce |
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a****k
发帖数: 1130 | 24 How to dissect autophagy with biochemical purification approach?? I think
the biggest problem for this field is that they don't have good assays to
measure autophagy. The requirement of an assay which is good enough for
biochemical purification is even higher. |
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n********k
发帖数: 2818 | 26 It is likely too late for a PHD or starting postdoc...I just found out last
night, a few of ideas I am planning on lncRNA have been worked OUT and just published in either cell, mole
cell etc...sure, there would be still a couple of ys to gold-rush in term of general mechanistic studies and
many ys ahead for functional dissections... and I am sure there are a lot more groups out there working on
lncRNA now... |
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l*****l
发帖数: 1451 | 27 Methods in Molecular Biology, 2009, Volume 545, 21-37, DOI: 10.1007/978-1-
60327-993-2_2
Functional Dissection of Mitotic Regulators Through Gene Targeting in Human
Somatic Cells |
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y******8
发帖数: 1764 | 28 One sample from google. Good luck!
Dear PLoS Biology editor:
I would like to submit a presubmission inquiry regarding suitability of our
manuscript, "Subversion of cellular autophagy pathway by RNA viruses" by
William T. Jackson, Thomas H. Giddings Jr., Sara Mulinyawe, Ron Kopito and
Karla Kirkegaard for publication in PLoS Biology. Cellular autophagy is a
field that is just entering a period of rapid discovery, because the
mammalian homologs for the many yeast genes that affect autophagy have v... 阅读全帖 |
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p*******r
发帖数: 4048 | 29 Yes, and chinese medicine.
I want to dissect the circuit, then develop a drug specifically for that
type of cell.
kind of like David Anderson, *blush*
interesting
one
the
a |
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n********k
发帖数: 2818 | 30 but the DPS may help the research and pinpoint where the problem is...As far
as I could think, DPS eventually
could well be a history as a therapy means but I see it potentially as a
great tool to dissect many outstanding
disease related questions before we could have other tools...say an
effective drug or whatever... |
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p*****m
发帖数: 7030 | 31 I highly doubt there is anything novel coming out (of your discussion with
puppeteer). In the field of neural
regulation of metabolism (incl. obesity), everyone knows the circuitry is
important and there are tons of
excellent papers out there in the past decades, dissecting pieces of
microcircuitry that regulate different
aspects of energy intake/expenditure... the optogenetics may add sth here (
like what DJA did), but again,
everyone is chasing after optogenetcs, what is your advantage? |
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w******a
发帖数: 1527 | 32 This is really interesting~~~ It may be able to dissect the human love in a
more objective way.
Sometimes girls can’t distinguish among true love, like, admiration,
dependence, etc， so this may help to diagnose their confusion…
Hope the 忘情水，孟婆汤 can come true~~~ |
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m*********r
发帖数: 136 | 33 I cannot get access to the database
y******[email protected]
Thanks |
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a********k
发帖数: 2273 | 35 这个。。。你确认你不是信口开河？
虽然俺不做fish imprinted gene，但是听说很多mammalian的imprinted chromosome的
synteny在鱼里面也都是保守的。当然别人paper作假另当别论。你那个imprinting的机制
，没有哪个能证明吧。。。至少俺是没看见过，等高人答疑。
鱼对于Xenopus的优势无非就是genetics那一点点，能够有很多的突变体，能够做
transgenic fish。injection难度忽略不计，因为每个人都能做，速度不比蛙慢。
microdisection会难很多，但是dissect出来那点点东西不能用来做imprinting吧。另
外就是xenopus个大，产卵却不比鱼多多少，卵黄太多，不太好做生化。
当然，鱼比起老鼠的那一点点优势也就在于能做发育早期一个很小的window，xenopus
在我看来是更没啥可取的，连那一点点window都因为不好做fluorescence消失殆尽。 |
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F*********n
发帖数: 2914 | 36 http://news.yahoo.com/s/digitaltrends/nasascientistfindsevidenc
Aliens exist, and we have proof.
That astonishingly awesome claim comes from Dr. Richard B. Hoover, an
astrobiologist at NASA’s Marshall Space Flight Center, who says he has
found conclusive evidence of alien life — fossils of bacteria found in an
extremely rare class of meteorite called CI1 carbonaceous chondrites. (There
are only nine such meteorites on planet Earth.) Hoover’s findings were
published late Friday night in the Journ... 阅读全帖 |
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w*******2
发帖数: 435 | 37 You don't need to cut the head off.\
you can use 91460-11 x1 to cut open skull from the top carefully without
damage the brain.Then take the whole brain off.
I have no idea why you need thin wax board.
We put 2% agarose in a small tissue culture dish.You can put the brain with
PBS in the dish for farther micro-dissection with tool 11255-20 x2
91500-09 x1.
good luck. |
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r*****m
发帖数: 231 | 38 Do you have someone to teach you how to do this? If you don't even know
which tools to use, I don't see the actual dissection as a trivial task for
you... |
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z****u
发帖数: 1007 | 39 microdissection是我们的daily work. 在dissection microsope下面解剖E10.5 mouse
embryo的first branchial arch..整个长度不超过2mm吧。然后把distal part ,
proximal part 分开，体外培养。
楼主需要的agar dish.2% agar加入碳粉让它变黑，然后autoclave之后铺petri dish.
black agar dish 背景解剖容易得多。
finescience的那些工具不错，我们一直都用它。
一定需要人带着做一遍。 |
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m*********n
发帖数: 215 | 40 stereomicroscope，那个有自带的黑色背景台。#10 surgical blade和＃5 镊子足够了。E12.5到p14都做过，在dish中加1Xpbs dissect。cerebellum, thalamus还有hippocampis都是这么明显的结构。。。 |
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l**s
发帖数: 55 | 41 try to dissect cartilage alone, also try 55 degrees (for both in situ and
wash) |
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m**********2
发帖数: 57 | 42 This is E15.5 bone tissue. It's difficult to dissect cartilage alone.
I will try the lower temperature first. Thank your suggestion.
Actually, I found the mineral zones in the middle attach firmly after
decalcification, while chondrocytes and hypertrophic chondrocytes are easier
to detach after in situ incubation compared to no decalcification tissues.
The pic is no decalcification tissues. |
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m****M
发帖数: 360 | 43 Can anyone send me a copy of the following paper?
Peptide scalpels for site-specific dissection of the DNA–protein interface
Thank you so much
m********[email protected] |
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c********r
发帖数: 1125 | 44
不止一篇pnas,一篇cell metabolism。。。
Zhou KM; Dong YM; Ge Q ... Xu T, PKA activation bypasses the requirement for
UNC-31 in the docking of dense core vesicles from C.elegans neurons, Neuron
, 2007, 56: 657-669.
Bai L, Wang Y, Fan JM... Xu T. Dissecting multiple steps of GLUT4
trafficking and identifying the sites of insulin action. Cell Metabolism.
2007, 5:47-57.
Kang LJ, He ZX, Xu PY, Fan J, Betz A, Brose N, Xu T. Munc13-1 is required
for the sustained release of insulin from pancreatic beta cells. C... 阅读全帖 |
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y******8
发帖数: 1764 | 48 You have to try the used one before you buy, because there is no money-back
policy. |
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n********k
发帖数: 2818 | 49 So using screening approach, how would you dissect interaction between the
organ? I know this is one very active area now...The Mango has a story
about FGF-something from Gallbladder regulate the intestine function/
homeostasis...
adipo |
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