|
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c*********i
发帖数: 674 | 3 中国一年出生两千万，一个收费五万，年收入就是一万亿，咋会不如马工 |
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w********2
发帖数: 632 | 4 三步
1.加sgrna
2.加cas9
3.加你想加的基因片段
都用pipette，加20ul，最好定量。 |
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p***n
发帖数: 17190 | 5 有個前輩跟我講 CRISP 後果不是只有你想修剪得那個
還會有其他很多的其他效應 |
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发帖数: 1 | 6 狗屁，排除crisp的潜在风险，基因编辑优化人类我觉得一点伦理问题都不存在。
以后中国人各个男的彭于晏 女的高圆圆 你说爽不爽 智商爱因斯坦。身高180+
你说爽不爽？
现在人反对不过是因为自己错过了好时候吧？
如果技术成熟，我第一个给孩子做基因编辑。
作伴 |
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p***n
发帖数: 17190 | 7 只有做過部分已知基因的篩選
crisp 最大的問題是
你把 DNA 兩股都剪斷了
那整個細胞會起動很大的反應
你並不知道因此接上去形成怎樣的基因序列
只做部份已知基因的篩選是沒法知道這些的
基改人應該做全基因定序
再去比對已知基因
這樣可以有效增加其中有問題的基因的篩選
要不然
人類基因庫中又多了好幾條病毒來的基因... |
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p***n
发帖数: 17190 | 8 我以前做生物晶片的
crisp的部份是該業界的前輩告訴我的
帝 區基改人 還吃基改食物
基改加基改
改上加改
怪不得全世界對帝區基改人要投以懷疑的眼神 |
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L*****d
发帖数: 5093 | 9 https://www.huffingtonpost.com/rodales-organic-life/the-top-10-tapwater-
cities-in-america_b_8184138.html
The Top 10 Tap Water Cities in America
09/23/2015 12:29 pm ET Updated Dec 06, 2017
What makes a great glass of water? A pristine source, plus careful testing
and high-tech filtration and treatment.
by Michael Park, for Rodale’s Organic Life
New York City
More than 1 billion daily gallons of sweet, mineral-rich water travels over
125 miles from carefully guarded Catskill Mountains reservoirs.... 阅读全帖 |
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s*f
发帖数: 1071 | 10 •Hamilton, Ohio – This city has won the Berkeley Springs
International Water Testing competition (locally nicknamed the “Academy
Awards of Water”) three times recently, in 2009, 2010, and 2015. Hamilton’
s only municipal water source is the Great Miami Valley Buried Aquifer.
•Clearbrook, Abbottsford, British Columbia – In the 2016 Berkeley
Springs International Water Testing event Clearbrook won the award for Best
Municipal Water in the world. The Clearbrook Waterworks District has w... 阅读全帖 |
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发帖数: 1 | 11
crisp 9应该弄不了那么长吧？
而且要往里插一段长的也很难 |
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v***t
发帖数: 27100 | 12 张峰,CRISP大拿, 比能教授年轻四岁, 现有组员20多人,不少白牛白弟 |
|
发帖数: 1 | 13 尽管张峰的crisp也是偷的
但尼玛张峰显然是诺奖level的
★ 发自iPhone App: ChinaWeb 1.1.5 |
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l**t
发帖数: 6971 | 16 【 以下文字转载自 USANews 讨论区 】
发信人: lopt (NextStep), 信区: USANews
标 题: McCain, Obama和Hillary的tax plan的比较
发信站: BBS 未名空间站 (Fri Mar 14 01:55:19 2008)
大家刚刚reveal details。
McCain：劫贫济富
Obama: 劫富济贫
Hillary: 罗罗嗦嗦，没有主题。研究半天才明白是benefit middle class。
Hillary一直就是缺乏一个crisp的message。她的policy，即使是好的policy，也很难让大众理解。
Hillary要搞各种各样的deduction和rebate。。。
Obama要大大提高long term capital gain tax。这个以前没有具体说过，现在说出来以后令我很失望。 |
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k*******3
发帖数: 918 | 17 Falun Gong sophister since July 1999 after the United States, more bad days,
one after another in the past ten years, leader Li Hongzhi promised a "
satisfactory" has become elusive, round heart on the brink of collapse. In
order to hold its ground, Falun Gong executives have called development
projects, while the leader Li Hongzhi concentrate on the "charm", trying to
charm into Falun Gong and trumpeter money grabbing hand. Unfortunately, the
"charm" XinBiTianGao, life than paper thin, but no o... 阅读全帖 |
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a*******k
发帖数: 74 | 19 This is really a great choice.
-This shows that he is really a maverick, making his own decisions.
-Also brings a change theme to his compaign.
-Use Palin to appeal to those still undecided female Clinton supporters.
-compensate for his old age and lack of executive experience.
Liked Palin instantly half way through her speech. She has working
classroots, and is not a washington insider. Her speech is clear and crisp.
She is energetic. Consevertive by reformist.
The election is getting more and |
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F********d
发帖数: 124 | 20 各大媒体和政治评论员，对第二次总统候选人辩论中麦凯恩的出色表现表示认同和赞赏，汇集整理如下：
Commentary Magazine’s Daniel Casse:
“McCain is delivering tight, crisp, and extremely effective answers.
Every answer has a similar structure: (a) I care about this issue (b) I’ve
stood up against Bush/special interests on this issue (c) Obama has never
taken a stand, never acted on this (d) so let’s compare records.”
==============================================
Balz, Kornblut, Abromowitz of the Washington Post weighed in:
McCain played th |
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C*******r
发帖数: 10345 | 21 As the Commander in Chief Deliberates, Frustration Builds Within the Ranks
WASHINGTON — Only nine months ago, the Pentagon pronounced itself reassured
by the early steps of a new commander in chief. President Obama was moving
slowly on an American withdrawal from Iraq, had retained former President
George W. Bush’s defense secretary and, in a gesture much noticed, had
executed his first military salute with crisp precision.
But now, after nearly a month of deliberations by Mr. Obama over whether |
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l****z
发帖数: 29846 | 22 By Dan Gainor
December 9, 2011
According to police, a letter bomb was recently sent to the CEO of Deutsche
Bank in Frankfurt, Germany. It was only a matter of time.
Left wing protesters – from the union loonies at SEIU to the equally loony,
but more radical Occupy Wall Street crowd – have been targeting bank
executives for some time. It was bound to happen that someone would take all
the venom aimed by the media and the left at so-called “banksters” and
turn it into a Unabomber homage.
The term ... 阅读全帖 |
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l****z
发帖数: 29846 | 23 Egypt’s New Leader Spells Out Terms for U.S.-Arab Ties
By DAVID D. KIRKPATRICK and STEVEN ERLANGER
CAIRO — On the eve of his first trip to the United States as Egypt’s new
Islamist president, Mohamed Morsi said the United States needed to
fundamentally change its approach to the Arab world, showing greater respect
for its values and helping build a Palestinian state, if it hoped to
overcome decades of pent-up anger.
A former leader of the Muslim Brotherhood and Egypt’s first democratically
elect... 阅读全帖 |
|
发帖数: 1 | 24 Michael Straw, a senior at Penn State University, returned to campus this
week, put on a crisp blue suit, and walked into a Trump buzzsaw.
The president of the school’s chapter of College Republicans had a sense of
what he was in for. Less than two weeks before, the group had announced in
a Facebook post that they would not endorse Donald Trump after holding an
online vote — a move that sparked outrage, including a call from the
chairman of the Pennsylvania Federation of College Republicans for ... 阅读全帖 |
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b********n
发帖数: 38600 | 25 https://genius.com/Ice-cube-black-korea-lyrics
[Blends into intro with dialogue from Spike Lee's Do the Right Thing]
"Twenty D Energizers."
"Twenty C Energizer?"
"D, not C, D."
"C Energizer?"
"D, motherfucker, D! Learn to speak English first, alright? D!"
"How many you say?"
"Twenty, motherfucker, twenty."
"Honey..."
"Mother fuck you!"
[Ice Cube]
Every time I wanna go get a fuckin' brew
I gotta go down to the store with the two
Oriental one penny countin' motherfuckers
That make a nigga mad enou... 阅读全帖 |
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t**x
发帖数: 20965 | 26 By 3:30 p.m. the massacre was well under way.[19] A group of women in Rock
Springs had gathered at the plank bridge, where they stood and cheered on
the rampage. Two of the women reportedly fired shots at the Chinese.[19] As
the riot wore on into the night, the Chinese miners scattered into the hills
, lying in the grass to hide. Between four and nine p.m., rioters set fire
to the camp houses belonging to the coal company. By nine p.m., all but one
Chinese camp house was burned completely. In al... 阅读全帖 |
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i****x
发帖数: 17565 | 27 你说的各方面都有，这个不好描述，只有开过好车才能体会到
最重要的可能还是转向，fun car转向特别crisp，指哪打哪。你仔细体会一般车的驾驶
过程，你转方向盘的一瞬间车先是侧倾，然后车头跟进，然后车尾才跟进，有两个延迟
。打正过程也是一样，车头先停止转动，然后车尾才正过来，然后整个车的侧倾消失。
侧倾延迟跟悬挂硬度有关，而车尾跟进的延迟跟前后轮距离（轴距）有关。fun car的
共同特征是悬挂较硬，侧倾小、第一个延迟几乎不存在，另外轴距不太长，所以第二个
延迟也很小。当你感受过那种方向盘转哪怕只转一丁点，身体都能毫无延迟马上感觉到
车也转了一丁点的灵巧，你就知道啥叫fun了。
更高程度上，当转向接近车极限时，一般车都会前轮略微打滑，车转动的方向比你想让
它转动的方向小，俗称推头，正规名称understeer。但是fun car的推头趋势都比较小
，就算车接近极限了，照样还是会按照你让它走的路线前进，给人感觉车辆极限很高，
而且很鼓励你逼近那个极限，这种成就感也是驾驶乐趣之一。
其他的还包括大马力，好听的引擎声音，顺滑清晰的手动档位等。贴个我玩车的视频，
凡是能大雨里还如此操控自如的... 阅读全帖 |
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t**********3
发帖数: 12623 | 28 MB P1 option comes with HK Logic7 with tuned-down bass considering music
taste of most MB owners. In reality, it sounds more crisp than BMW's Logic7.
But they're both crap, and don't sound better than base stock - I don't
think those are Bose.
AMG cars are equipped with Bang & Olufsen, so much better sounding. |
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g********d
发帖数: 19244 | 29 http://www.motorward.com/2012/12/toyota-gt86-named-top-gear-mag
The amazing Toyota GT86 wins another major accolade as Top Gear Magazine
names it their 2012 Car of the Year. These guys know a thing or two about
what makes a car truly great, and the GT86 must have that in spades to
convince them that it抯 the winner.
This affordable Japanese sports car is also Jeremy Clarkson抯 personal Car
of the Year and the TopGear Coupe of the Year. They are blown away by the
amount of fun the GT86 delivers for... 阅读全帖 |
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f******t
发帖数: 7283 | 30 不参加争吵，这里提供一些关于MDX的信息：
1. 很多人给MDX和TL（SH-AWD版）做过dyno，四个轮的轮上马力大概在280多左右，网
上可以查到很多dyno实测结果，最好的甚至轮上就已经超过300马力。种种迹象表明
Acura低标了马力。从我实际试开TL和MDX的感觉来讲，还有各媒体实测MDX的加速成绩
，我不太相信它们的实际输出只有厂家标称的那个数值。
2. 一些关于那个3.7L V6的信息，抄自官方新闻稿：
ENGINE ARCHITECTURE
The MDX's 3.7-liter V-6 engine employs an inherently smooth-running 60-
degree V-angle. The cylinder block is constructed of aluminum and has high-
silicon aluminum cylinder liners for added weight savings and excellent
cooling. The cylinder heads are cast of lightwei... 阅读全帖 |
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g******e
发帖数: 3760 | 31 The 2010 model year marked the last of the sixth-generation Volkswagen
Passats. Cast in the hyper-competitive mid-size sedan segment, the Passat
differentiated itself from the Camrys and Accords by bringing larger
dimensions, crisp styling and a high-end interior that made friends easily.
It also offered a five-door wagon, a rarity in the category.
Most Passats came with Volkswagen’s 200-hp 2.0 L turbocharged four cylinder
; optional was the 280-hp 3.6 L VR6. Both engines were early adopters of
... 阅读全帖 |
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g********d
发帖数: 19244 | 32 ☆─────────────────────────────────────☆
lilyamao (lilyamao) 于 (Sat Feb 9 22:51:07 2013, 美东) 提到:
前段时间版上嘀咕沃而沃这车没人买什么的，当时就想上来吭一声，这volvo在我们东
北疙瘩还是横行天下的，路上很多见。
这车我开了两年多了，买的时候，仔细根mdx比较了一下，两个车价格差不多，但是同
样价格，mdx配置要好一些，尤其是mdx的内部装修，看上去volvo要好，能方东西的地
方多，这volvo的设计吧，小处上真是没人性， 到现在我都不知道纸巾盒子可以放哪里
，真是的，在车里丢来丢去，最后这盒子总是惨不忍睹。
当时驾驶的时候，操作感也是mdx轻松，动力感觉也是mdx好
至于为什么最后选择了volvo呢， 也没有什么理由，就是年少时候喜欢欧洲车，就这么
着，最后还是奔着年少时候的一个梦想而去了。
开了两年多了，总体感觉还好，没有什么毛病，就是换轮胎的时候小心痛了一下， 两
万多迈就要换了， 一换还得1200美元左右，我以前的mazda，5，6万迈才换一次轮胎，
换一回，好像才40... 阅读全帖 |
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x*b
发帖数: 5723 | 33 这种设计就是明显为了更好的操控和风阻不惜牺牲空间而转向coupe化，这个只有
Porsche敢这么做啊。
转一些专业评论:
Three engine choices at the introduction:
Macan S: 3.0 V6 B-Turbo 340hp, 460NM (340lbs-ft)
Macan Turbo: 3.6 V6 Bi-Turbo 400hp, 550NM (405 lbs-ft)
Macan S Diesel: 3.0 V6 258hp, 600NM (442lbs-ft)
- 0-62mph for Macan Turbo: 4.5 sec
- 4-cylinder engines arrive in 2015 (Both petrol and diesel)
- Much lower center of gravity than Audi Q5 (Macan is an inch lower),
despite being slightly heavier than Q5.
- They call it the most agile SUV ever... 阅读全帖 |
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O*********h
发帖数: 4674 | 34 最近旧车一堆毛病，dump给carmax了，carmax给了个很好的价，比craigslist 拿的
offer一样
比dealer多3000
马上会commute比较远，一年估计会有2w2-2w5 miles吧
328i 高速大家一般说34没问题
fuelly combine 28.8
328d fuelly combined 38
tdi passat fuelly combine 37
算了一下，貌似一年汽油vs柴油差价 670左右
现在328i价格便宜不少， 328d没的选，要么买很高配的，要么custom order，坏处就
是要等3-4个月
现在等车用，去hertz租3个月1500
tdi现在有高配的车，3w otd应该没太大问题
自己喜欢一直开bmw，这代F30 3我开了感觉虽然的确是没以前那么crisp，但是还算很
balance
实惠貌似是tdi实惠多了，车大，省油，还便宜，内饰凑合
明天准备下手，大家给个建议 |
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i****x
发帖数: 17565 | 35 不要脸的婊子媒体CR，拿了福特和通用的钱就把它们选成首选车型了，我呸！
Consumer Reports’ 2016 Top Picks By Category:
MIDSIZED CAR: Toyota Camry ($24,089-$32,603) The Camry’s no-fuss driving
experience – great outward visibility, controls that fall easily to hand, a
roomy interior – may not be the most thrilling in its class, but it’s far
from plain. The solid Camry delivers year after year of outstanding
reliability, which when combined with impressive crash-tests results, make
it a near-perfect sedan.
SUBCOMPACT CAR: Honda Fit (... 阅读全帖 |
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W***n
发帖数: 11530 | 36 Full Expert Review: 2017 Chrysler Pacifica
What's New for 2017
The 2017 Chrysler Pacifica is an all-new minivan. In effect, though, it's
the replacement for the previous Town & Country minivan, which Chrysler has
discontinued.
Introduction
If you've either been a passenger in or owned a steady flow of Honda
Odysseys and Toyota Siennas throughout your life, you are forgiven for
thinking that Chrysler minivans have largely existed in a state of perpetual
rental fleet mediocrity. While Chrysler was... 阅读全帖 |
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b****l
发帖数: 97 | 37 双离合目前里面最顺滑的是vw的，特别是新A4的7速，0-60时间直接爆炸。
AT最好是zf 纵置8速，crisp & fast，宝马全系，捷豹路虎，大切，rolls royce都用
。爱信的横置8速也还可以，就是出得早成本低，换挡速度远不如zf 8at，volvo和丰田
系用的多。福特和gm的新9at挺一般的，当然比zf那个9at好，dog clutch坑爹。 |
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f*****h
发帖数: 4489 | 38 双离合加速确实厉害 但低速顿挫只能改良 要达到at的水平貌似无解？
zf8速这么牛逼 为啥9速就不行了 偏偏本田的pilot就买他的9速 为啥呢
大众为啥喜欢买丰田的爱信 而不去买zf 是因为便宜吗
高手能否谈谈手动和cvt哪家好？
：双离合目前里面最顺滑的是vw的，特别是新A4的7速，0-60时间直接爆炸。
：AT最好是zf 纵置8速，crisp & fast，宝马全系，捷豹路虎，大切，rolls royce都用 |
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W***n
发帖数: 11530 | 39 wonderful mtn cruising yesterday
t air is so crisp
t views r so nice |
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x*********n
发帖数: 28013 | 40 好便宜。
我一般都是3块多一磅，最贵一次买过4.99一磅的， |
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s****g
发帖数: 5 | 41 Electric Appliance
Canon PIXMA MP490 Inkjet Photo All-In-One Printer
$47
Like new with great condition.
Product Dimensions: 17.8 x 13.2 x 6.1 inches ; 14.6 pounds
Technical Details
• Use the new Auto Photo Fix II feature to automatically adjust and
correct your photos
• The ChromaLife100+1 system gives you beautiful and long-lasting
photos
• Collect, combine and edit multiple web pages to print your own
layout using Easy-WebPrint EX
• Features pigment black in... 阅读全帖 |
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d*****0
发帖数: 141 | 42 现出售全新未拆封的佳能彩色照片打印机一台，市价$399.99，甩卖价$300
洛杉矶地区优先。可以当场验货，只接受现金或者支票。
纯粹个人用品，保证真货。
Canon PIXMA iP4700 Premium Inkjet Photo Printer
Technical Details
Incredible 9600 x 2400 color dpi1 for the best in photo lab printing
Print amazing 4 x 6 inch borderless photos in approx. 20 seconds
The ChromaLife100+ system gives you beautiful and long lasting photos
Keep It Green - Built-in 2 sided printing helps the environment and cuts
your paper usage by 50%
Do even more with the included software from easily pri... 阅读全帖 |
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s********8
发帖数: 440 | 43 $150/each, Your label
Canon PowerShot SD4500 IS Silver Bundle w/ 8GB Memory, Reader, Case, Mini
Tripod, More
Condition: Brand new, USA Warranty
Kit Includes:
Canon - PowerShot SD4500IS 10MP Digital Elph Camera w/ 10x Optical Zoom
1080P HD Video
Full HD 1080P Video
Compact Design w/ 10x Image stabilized zoom
Canon's HS System: 10MP CMOS Sensor DIGIC 4 Image Processor
3 inch LCD
HDMI output
General Brand - Hi-Speed 8GB Secure Digital SD Memory Card
Perfect for expanding your camera memory to tak... 阅读全帖 |
|
s********8
发帖数: 440 | 44 $130/each, Your label
Canon PowerShot SD4500 IS Silver Bundle w/ 8GB Memory, Reader, Case, Mini
Tripod, More
Condition: Brand new, USA Warranty
Kit Includes:
Canon - PowerShot SD4500IS 10MP Digital Elph Camera w/ 10x Optical Zoom
1080P HD Video
Full HD 1080P Video
Compact Design w/ 10x Image stabilized zoom
Canon's HS System: 10MP CMOS Sensor DIGIC 4 Image Processor
3 inch LCD
HDMI output
General Brand - Hi-Speed 8GB Secure Digital SD Memory Card
Perfect for expanding your camera memory to tak... 阅读全帖 |
|
s********3
发帖数: 31 | 45 Moving sale:
This TV is pretty new with great performace (please check it out by the
attached pictures)! I brought it half of years ago with the orignal price is
$380. The features is as the blow:
HD 720p resolution panel
Enjoy crisp, detailed and vibrant images in 720p picture quality while
watching movies, sports and primetime HD television broadcasts via the 16:9
wide screen panel (1366 x 768).
Digital noise reduction
Cleaner, clearer, more vivid pictures from all inputs, even USB and PC! Now... 阅读全帖 |
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a****n
发帖数: 18 | 46 我们组招经理，有兴趣的同学请给a**********[email protected]写信投简历。如果有认识的
人适合该职位，也请相互refer.
http://www.amazon.com/gp/jobs/177886/
Investigations operations manager, Amazon China
US, WA, Seattle • Job ID 177886 • Amazon Corporate LLC
Job Description
Join one of the most important teams at Amazon and make a difference. TRMS
is charged with making Amazon.com/Amazon.cn the safest place to transact
online. We achieve this through a combination of automated and manual
investigations of the buyers and ... 阅读全帖 |
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a****n
发帖数: 18 | 47 我们仍然在招人，上次贴过了，由于这个职位需要的headcounts很多，所以长期都有效
。唯一有变化的是，我们现在接受opt。所以感兴趣的同学，给amazontrmscn@gmail.
com写信投简历。
http://www.amazon.com/gp/jobs/222555/
Risk Investigator - Chinese Language
US, WA, Seattle • Job ID 222555 • Amazon Corporate LLC
Job Description
Amazon.com is seeking dedicated, hardworking, analytical candidates with a
proven track record of performance and results-oriented thinking, to join
the Risk Investigations Team. Candidates will be responsible for a wide
range of duties re... 阅读全帖 |
|
j*******n
发帖数: 2205 | 48 Dell V7140PRO-4655BLK - Venue 11 Pro 10.8" - Intel Core M - 64GB - with
Keyboard - Black
Boost your on-the-go productivity with this Dell Venue 11 Pro tablet, which
features Wi-Fi for easy Web access and a 64GB capacity that provides space
for important files. The 10.8" capacitive touch screen simplifies use and
offers crisp images.
Windows 8.1 64-bit
10.8" high-definition 10-point capacitive touch screen with 1920 x 1080
resolution
64GB storage capacity
Dual-core processor Intel® Core®... 阅读全帖 |
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w*******y
发帖数: 60932 | 49 【 以下文字转载自 DealGroup 俱乐部 】
发信人: wiiRhappy (wiiRhappy), 信区: DealGroup
标 题: PowerBar Performance Bars - $5.42 12ct/box shipped
发信站: BBS 未名空间站 (Mon May 31 15:05:55 2010, 美东)
GNC.com has PowerBar Performance Energy bars in 12ct/boxes for $7.97 - 20%
off protein bars = $6.38.
Free shipping for today. Potential stack with Bing cashback.
This comes out to 53 cents a bar.
PowerBar Performance Energy Bar - Vanilla Crisp
Link:
http://www.gnc.com/product/index.jsp?productId=2636164&clickid=prod_cs
PowerBar |
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