g*****n
发帖数: 250 | 1 上一半说对了。EDTA是用来chelate二价金属离子的.
下一半不对. EDTA不会导致蛋白水解. 加EDTA是一种防范措施。DNA酶需要镁离子。一
旦DNA溶液被DNA酶污染，EDTA可以抑制酶活性。 |
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w***r
发帖数: 709 | 2 这个人太牛X了。他在song h 甲基化文章真的很炫!！
但是我个人觉得
Cytochrome P450 drives a HIF-regulated behavioral response to reoxygenation
by C. elegans. Science (2013) Vol. 341 no. 6145 pp. 554-558. PMID: 23811225
这篇文章似乎新意不够，hif 和p450和缺氧的关系早就知道了。下面是一篇，还有不少
有明白人讲一讲吗？
Inhibition of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-stimulated Cyp1a1
promoter activity by hypoxic agents.
Kim JE1, Sheen YY.
Author information
Abstract
Since hypoxia-inducible factor-1alpha (HIF-1alpha) and the arylhydrocarbon
receptor (AhR) shared th... 阅读全帖 |
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w***r
发帖数: 709 | 3 这个人太牛X了。他在song h 甲基化文章真的很炫!！
但是我个人觉得
Cytochrome P450 drives a HIF-regulated behavioral response to reoxygenation
by C. elegans. Science (2013) Vol. 341 no. 6145 pp. 554-558. PMID: 23811225
这篇文章似乎新意不够，hif 和p450和缺氧的关系早就知道了。下面是一篇，还有不少
有明白人讲一讲吗？
Inhibition of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-stimulated Cyp1a1
promoter activity by hypoxic agents.
Kim JE1, Sheen YY.
Author information
Abstract
Since hypoxia-inducible factor-1alpha (HIF-1alpha) and the arylhydrocarbon
receptor (AhR) shared th... 阅读全帖 |
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a*****r
发帖数: 54 | 4 胖老师，HIF field用的最多的HIF1A的抗体是BD的#610958（鼠单抗）,WB的band在～
120kD
在normoxia培养条件的cell lysate信号几乎没有或者很低
需要进行一定的处理才能看见robust的信号，例如
(1)MG132 ~2hr
(2)CoCl2/iron chelator（比如DFO）
(3)PHD inhibitor（比如DMOG）
(4）如果你有Hypoxic incubator,用低于2% O2
(5)或是直接使用某些Mutant细胞，例如RCC4 (VHL inactivated) |
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d****i
发帖数: 360 | 5 Preparation of porous chelating resin containing linear polymer ligand and
the adsorption characteristics for harmful metal ions , Reactive and
Functional Polymers, Volume 53, Issues 2-3, December 2002, Pages 91-101
Margot A. Llosa Tanco, David A. Pacheco Tanaka, Veronica C. Flores, Takako
Nagase and Toshishige M. Suzuki
E-mail: x**********[email protected] |
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d*******g
发帖数: 3972 | 6 how about Zn(BH4)2？ Pretty good for chelate controlled reduction |
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P********n
发帖数: 372 | 8 Title: Laboratory Technician/Chemist
Location: Houston TX, 77082
Job Summary:
· The technical service /development chemist will provide hands-on
laboratory support to solve various customer problems and generate
innovative technical solutions that support current business and generate
new business growth opportunities
Specific duties include:
· Assisting in the technical transfer of new technology/product and
technical service request for a Global Key Account
· Conducting... 阅读全帖 |
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m*******m
发帖数: 173 | 9 There was a saying I heard from a mom: 6 weeks of chelation works better
than 2 years of ABA. That was my point: Outside training must be
accompanied by inside remedy. That's the way to solve problems from their
origins.
Thanks for your warm hearted reminder though. I know what you mean. He is my son, I will do my best.
scientifically |
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a*****a
发帖数: 210 | 10 carboxylic acids chelates with Al3+.
Your CMC(carboxylmethyl cellulose) is very possible have free carboxylic
acid especially when you made it acidic.
In Pressure Sensitive Adhesive industry, Al3+ is used to gel polymer
containing free carboxylic acid.
Acetylacetone can stablize the Al3+. After addition of enough Acetylacetone,
Al3+ will not gel carboxylic acid containg polymer at room temperature.
However, if you evaporation the Acetylacetone, you will get gel. |
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a*****a
发帖数: 210 | 12 carboxylic acids chelates with Al3+.
Your CMC(carboxylmethyl cellulose) is very possible have free carboxylic
acid especially when you made it acidic.
In Pressure Sensitive Adhesive industry, Al3+ is used to gel polymer
containing free carboxylic acid.
Acetylacetone can stablize the Al3+. After addition of enough Acetylacetone,
Al3+ will not gel carboxylic acid containg polymer at room temperature.
However, if you evaporation the Acetylacetone, you will get gel. |
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W***M
发帖数: 50 | 14 In the US, it is usually treated with chronic transfusion and iron chelation
. The risk for unrelated bone marrow donor is high, including death, chronic
graft vs host disease, severe immune supppression and risk of infection,
and secondary cancer. The risk is higher with unrelated donor. The Italians
did the most transplant using unrelated donor. Most center here is the US
will do transplant using matched siblings. Also, it is not a surgery. If
transplant failed, it is death. |
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W***M
发帖数: 50 | 15 In the US, it is usually treated with chronic transfusion and iron chelation
. The risk for unrelated bone marrow donor is high, including death, chronic
graft vs host disease, severe immune supppression and risk of infection,
and secondary cancer. The risk is higher with unrelated donor. The Italians
did the most transplant using unrelated donor. Most center here is the US
will do transplant using matched siblings. Also, it is not a surgery. If
transplant failed, it is death. |
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e****0
发帖数: 678 | 16 • leukemia
ALL AML CLL
child adult elderly
BM lymphoblasts 25%myeloblasts
lab Lymphocyte 5000, mature-appearing cells
• Osgood-schlatters disease
Adolescent male athletes
Traction apophysitis—quadriceps tendon put the traction on the
apophysis of the tibial tubercle where patellar tendon inserts.
A firm mass =heterotopic bone formation
Pain can be reproduced by extending the kne... 阅读全帖 |
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b*****e
发帖数: 62 | 17 来自主题: Pharmaceutical版 - 问就业前景 这个方向neurotoxic metals, their toxicokinetics and chelation. Specific the
role of physico-chemical properties of nanoscale materials in their ability
to enter the brain
另外一个方向研究obesity和 cardiovascular disease.
主要是用knock out mice，然后就是细胞什么的
不知道这两个方向哪个比较好就业呢？ |
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u*******s
发帖数: 688 | 18 暑假开始持续作战到现在。这是第一个能休息的周末，来随口提
提我的retail感想。
我在药房里主要负责的就是production和register. 本来还想学学billing，后来仔细
想想，其实作为Pharmacy intern，最应该学习和熟练的就是如何配药和如何与病人打
交道。
有retail经验的大概都知道，filling是许多人都不愿意做的——枯燥，乏味，count
by 5直至头晕眼花。我在这几年多多少少的工作中，一开始也是这么想的。后来也渐渐
悟出filling过程中可以用心的地方：
1）最基础的，当然是brand-generic
2）拿到一张label，通过上面所写的剂量，能了解一些病人大概的什么情况？当然我说
的不是慢性病的药。
3）如果一个病人有若干处方，我一边配，一边会想想有这里有没有什么drug
interaction
4）什么药可以cut，什么药不行 ——这个比较简单，当然也有相对比较tricky的。药
品名称带有XL,SR,ER，有些也可以cut，根据药物的施放原理不同，你可以比较什么可
以cut,什么不行。
5）有些药是cytotoxic，最好不要皮肤接... 阅读全帖 |
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j*****e
发帖数: 236 | 19 非常认真负责！大赞！
暑假开始持续作战到现在。这是第一个能休息的周末，来随口提
提我的retail感想。
我在药房里主要负责的就是production和register. 本来还想学学billing，后来仔细
想想，其实作为Pharmacy intern，最应该学习和熟练的就是如何配药和如何与病人打
交道。
有retail经验的大概都知道，filling是许多人都不愿意做的——枯燥，乏味，count
by 5直至头晕眼花。我在这几年多多少少的工作中，一开始也是这么想的。后来也渐渐
悟出filling过程中可以用心的地方：
1）最基础的，当然是brand-generic
2）拿到一张label，通过上面所写的剂量，能了解一些病人大概的什么情况？当然我说
的不是慢性病的药。
3）如果一个病人有若干处方，我一边配，一边会想想有这里有没有什么drug
interaction
4）什么药可以cut，什么药不行 ——这个比较简单，当然也有相对比较tricky的。药
品名称带有XL,SR,ER，有些也可以cut，根据药物的施放原理不同，你可以比较什么可
以cut,什么不行。
5）有些药是cytot... 阅读全帖 |
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m******h
发帖数: 297 | 20 友情纠正一下几个事实，那个医生是Dr. Rashid A. Buttar，有没有执照大家可以
google。这个医生用的是chelation therapy，所谓的detox，他从来没有用过高压氧。
也没有任何报道Desiree Jennings被用高压氧治疗过。 |
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g****x
发帖数: 17 | 21 谢谢
牙病的起源与治疗
(2013-11-19 06:04:20) 下一个
作者： 蜀人
牙病与我们的身体健康息息相关。中国的一句老话说：“挑马勘牙，选郎见齿”。意思
是一个有经验的马夫挑选一匹好马，首先是看马的牙齿好坏。如果要判定一个人是否健
康，也应该第一眼观察他的牙齿状况。一个牙不好的人，健康肯定有问题。这句话是人
们长期经验的总结，那么是否有确切的科学依据呢？答案是肯定的。据研究人的口腔含
有大约500多种细菌，其中一些是有害细菌，会引起牙齿与牙龈感染发炎。但是这些大
量繁殖的有毒细菌并非静静地呆在口腔不动，它们会趁机循着血流到达身体的各个部位
。当它们迁移时，如果我们的免疫力不足，就会感染心脏，肾脏，关节，神经系统，大
脑，眼睛，实际上可能会感染任何器官，腺体或其他组织。据研究报告可能会导致数以
百计的不同疾病。
人们普遍认为，有牙病自然而且当然是看牙医了。但是现在的牙医真的可以帮助你吗？
我的答案是：No! 或者说即使有帮助也非常有限，而且手术痛苦，副作用大，要价昂贵。
花钱受罪 苦寻答案：
对现代牙医技术的质疑完全起源于我的个人经历。过去我的牙齿一直不太好，牙敏感，
牙... 阅读全帖 |
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