w***a
发帖数: 4361 | 1 糖尿病field，有一种说法是adipose tissue在快速增殖的时候，angiogenesis跟不上
obesity的expansion，造成local hypoxia。而hypoxia引起inflammation，
然后recruit macrophage，inflammation继续加重。
这种恶性循环最后导致global insulin resistance 。 |
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p*****m
发帖数: 7030 | 2 代谢和免疫？都不算是worm/fly的太好的用武之地吧 除非你有很specific的问题要问
并且别的系统不好回答。比如说你要搞pathogen/host interaction 这个worm/fly好歹
还是活得动物；之类的。
worm/fly都没有adaptive immune system（虽然要说起来Toll还是果蝇里clone的）。
worm/fly也都没有正儿八经的mammalian代谢的遗传/组织元素。比如说两者都没有正儿
八经的insulin（只有ILP）；都没有liver, adipose tissue，就算是有类似的组织功
能也不是那么一样；果蝇幼虫和线虫都没有控制食欲的神经系统，基本就是一辈子吃到
死的意思。。 |
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p*****m
发帖数: 7030 | 3 嗯 挺多neuro+metabolism，不过我是觉得做得不够好了，尤其是老鼠里。如果你比比我
们对peripheral metabolism (in adipose, liver, muscle, pancreas, etc)的理解和
对brain的理解 能差个数量级吧感觉。
呵呵 还真有人interview问我这个问题。。其实我从来就没想过继续做的说。。所以也
就压根没和他讨论过。。。觉得原来的话题太小众了 不好玩。。
why
something?
it
,
are still young:))) |
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w***a
发帖数: 4361 | 4 apoptosis了以后就release free fatty acid，然后macrophage也来了，
然后又是inflammation什么的，情况就越来越糟糕。
adipose tissue在身体里面的作用就跟加油站，炼油厂差不多。
平时管理好了，相安无事。
但是加油站要是炸了，energy都给瞬间释放了，肯定没什么好事情。 |
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O*D
发帖数: 17 | 5 有2个postdoc offer，
1） 方向是GWAS，主要是验证某个SNP和某疾病的相关性。经费未来5年都比较充足。学
校是某一大牛校。PI是此疾病领域的临床医生，应该在该疾病领域比较牛。
2） 方向是脂肪细胞分化，主要研究brown adipose tissue分化。经费未来5年充足。
学校是中牛校，PI是AP。
1）的优点是大牛校，PI也牛，缺点是GWAS这个领域水分比较大，不知道对我未来的
faculty前景是否会有不利影响，另外本人也不大喜欢functional genetics，感觉这个
领域很容易做到头。不知道各位对该领域有何看法。
2）的优点是领域比较热，研究前景似乎不错，缺点是PI是新AP，目前还没有好的文章
发表，虽然PI的博后老板是大牛，博后发的文章也不错。也就是说有一定的风险。本人
倾向2），但是因为博士期间做的是癌症相关的研究，对上述领域都不是非常熟悉。希
望懂行的人指点。
谢谢。 |
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s*********n
发帖数: 13 | 6 Position: Postdoctoral Scientist in Diabetes.
The diabetes and obesity group of our company in Boston is committed to
discovering innovative medicines to treat cardiovascular and metabolic
disorders. We have an immediate opening for a postdoctoral scientist in the
areas of metabolic, cardiovascular, and mitochondrial dysfunction research.
The postdoctoral scientist will be responsible for:
Validating novel targets for diabetes, insulin resistance, and
metabolism in skeletal muscle, l... 阅读全帖 |
|
s*********n
发帖数: 13 | 7 Position: Postdoctoral Scientist in Diabetes.
The diabetes and obesity group of our company in Boston is committed to
discovering innovative medicines to treat cardiovascular and metabolic
disorders. We have an immediate opening for a postdoctoral scientist in the
areas of metabolic, cardiovascular, and mitochondrial dysfunction research.
The postdoctoral scientist will be responsible for:
Validating novel targets for diabetes, insulin resistance, and
metabolism in skeletal muscle, l... 阅读全帖 |
|
t*d
发帖数: 1290 | 8 真是有钱人会更有钱
Exercise benefits a variety of organ systems in mammals, and some of the
best-recognized effects of exercise on muscle are mediated by the
transcriptional co-activator PPAR-γ co-activator-1 α (PGC1-α). Here we
show in mouse that PGC1-α expression in muscle stimulates an increase in
expression of FNDC5, a membrane protein that is cleaved and secreted as a
newly identified hormone, irisin. Irisin acts on white adipose cells in
culture and in vivo to stimulate UCP1 expression and a broad ... 阅读全帖 |
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b*******n
发帖数: 605 | 9 Is this related to your KO? |
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j****t
发帖数: 1663 | 10 单单控制心血管不能防治衰老！其他tissue（e.g.adipose tissue）也许可以。
但研究心血管aging，可以找到防治心血管疾病的办法。 |
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l**********1
发帖数: 5204 | 11 上周偶然撞到一篇review
说的就是这个
pls go to
Yadav H. at al. (2012)
TGF-β/Smad3 Signaling Regulates Brown Adipocyte Induction in White Adipose
Tissue.
Front Endocrinol (Lausanne). 3:35.
PubMed link:
//www.ncbi.nlm.nih.gov/pubmed/22654861
发信人: Dua (Dua), 信区: Biology
标 题: Re: 有个很有意思的发现，大家看看该怎么继续下去--METABOLISM
发信站: BBS 未名空间站 (Sun Jun 17 11:35:04 2012, 美东)
kindey附近还有啊？从来木有见到啊，下次仔细看看。。。
。
肩胛骨那里是不是一直连到腋窝那里去的？腋窝下面还有一点？ |
|
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y****i
发帖数: 2194 | 13 你说的那些应该是皮下脂肪
BAT在肌肉层下面，你可以去找cinti的那本adipose organ的书 画的很明白 |
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w***a
发帖数: 4361 | 14 muscle specific insulin receptor KO mouse
这个老鼠的肌肉是insulin resistance，但是整个老鼠并不insulin resistance。
是个比较奇怪的mouse model。
相对靠谱的解释是说，adipose tissue变得更加insulin sensitive，
然后就起到了compensation的作用。 |
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l**********1
发帖数: 5204 | 15 LZ
please refer
Xu Y et al. (2010)
Connective Tissue Growth Factor in Regulation of RhoA Mediated Cytoskeletal
Tension Associated Osteogenesis of Mouse Adipose-Derived Stromal Cells.
PLoS One. 5: e11279.
http://www.ncbi.nlm.nih.gov/pubmed/20585662
its Fig. 2 B d |
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x**4
发帖数: 112 | 16 http://www.ncbi.nlm.nih.gov/pubmed/22147018
Circadian feeding drive of metabolic activity in adipose tissue and not
hyperphagia triggers overweight in mice: is there a role of the pentose-
phosphate pathway?
Endocrinology. 2012 Feb;153(2):690-9. Epub 2011 Dec 6.
Thanks |
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y**u
发帖数: 7459 | 17 一般用white adipose tissue, 腹腔下部两大片 |
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f***x
发帖数: 1524 | 18 check TG in liver ,serum and adipose tissues.
check free fatty acids and cholesterol also.
if abnormal,check genes involved in lipogenesis and lipolysis. |
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z*****n
发帖数: 444 | 19 I think what we want to do is targeting the thermogenesis pathway
specifically to reach a status in which you generate modest heat but do not
disturb other physiological functions, such as enzyme activity. The heat
could be from brown fat, beige fat or white fat, which may all involve the
sympathetic nerve activity through beta 3 adrenergic receptor, cAMP pathway.
Sympathetic activity independent activation of adipose tissue is possible,
such as an significant increase of UCP1, which may be able... 阅读全帖 |
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z********8
发帖数: 818 | 20
只知道有跨膜结构，在adipose 和 mucsle 表达。 |
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c***r
发帖数: 1132 | 21 直接bank细胞，viablity是个大问题。
现在有tissue bank（adipose bank，or fat bank）， 可以bank tissue，然后需要的
时候，再化冻tissue，从中提取干细胞。 |
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m******g
发帖数: 467 | 22 Nature 14.03.13
推荐：
1. Evolutionary biology: Sex, lies and butterflies
doublesex is a mimicry supergene
稍微了解一下：
1. Evolutionary biology: Speciation undone
large finch has become extinct through interbreeding with another
small and medium tree finches on Floreana may also be fusing into one
species
2. DNA-guided DNA interference by a prokaryotic Argonaute
a barrier for the uptake and propagation of foreign DNA
TtAgo is loaded with 5′-phosphorylated DNA guides, 13–25 nucleotides in
length
小知识：
1.... 阅读全帖 |
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I*******U
发帖数: 869 | 24 最近要提取从白色脂肪里面提取SVF，根据protocol我确实看到了pellet，用TRI-
reagent也提取到了mRNA,nanodrop测出的浓度有800ng/ul，但是我试了cyclophilin，
gapdh，18s几个内参，都测不到CT值。已经重复几次了，郁闷的不行。请教各位，有什
么建议么？
SVF protocol
1）isolate WAT,
2) Incubate tissue @ 37C for 45min with shaking @ 80 rpm （Collagenase I，
1mg/ml）
3）300g for 10min
4) Dissolve pellet in Tri-reagent. |
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|
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z****u
发帖数: 1007 | 27 换一下酶。我个人感觉collagenase不够强。组织解离不够。 我用collagenase 2mg/ml
+dispase. 或者直接上trypsin LE 都可以。 |
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I*******U
发帖数: 869 | 28 现在不是组织解离不够，我能拿到细胞，也能测到RNA 浓度，可是housekeeping gene
18s，GAPDH和Cyclophilin都测不出，这个是什么原因？
楼上的你有没有测过SVF的gene表达呢？用什么housekeeping gene呢？谢谢
ml |
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m***o
发帖数: 272 | 29 RNA 跑跑胶看看.如果housekeeping 都测不出,你拿到的那就不是RNA,或者是降解了.如
果RNA 确定没问题,是不是PCR 的reagent有问题了?拿一个阳性RNA 来测.一步一步的排
除,还能找不到原因? |
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L****e
发帖数: 499 | 30 Curr Opin Lipidol. 2014 Feb 22.
Comparative gene identification-58/α/β hydrolase domain 5: more than just
an adipose triglyceride lipase activator? |
|
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s*******e
发帖数: 1389 | 32 The last paragraph of this paper:
The apparent divergent activities in vivo now ascribed to FGF1 seem to
closely parallel those of FGF21, which has been assigned locally restricted
roles in adipose tissue as well as systemic glucose-lowering activities.
However, FGF21 circulates as a true endocrine hormone, whereas the high-
affinity heparan sulphate proteoglycan-binding activity and serum lability
of FGF1 restrict its endogenous actions to local tissues, resulting in the
rapid clearance of exog... 阅读全帖 |
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e*****n
发帖数: 139 | 33 Oh, No.
Insulin sensitization would result in increase in RER, which shows the
elevation of carbohydrate oxidation and the concomitant reduction of fat
oxidation. Therefore, the overall energy expenditure would not be altered.
If food intake is not affected, adiposity and body weight will then be
maintained. The increased glucose taken into cells will get burned,
replacing the elevated fatty acid oxidation in diabetic state. The
biochemical mechanism underlying glucose/fatty acid fuel selection ... 阅读全帖 |
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e*****n
发帖数: 139 | 34 现在好多所谓的大paper 完全违反生理学原理，没什么意义。 Please keep in mind,
energy demand determines energy expenditure, and NOT the other way around!
Without the increase in energy demand, a mere expansion of oxidative
capacity, such as induction of browning of WAT, will not do anything!
Browning of WAT is operative under cold because the body energy demand under
cold stress is increased (to maintain temperature homeostasis). I don't
believe the idea of treating metabolic syndrome by inducing browning. Unless
energy... 阅读全帖 |
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v*******a
发帖数: 759 | 35 A New Era in Brown Adipose Tissue Biology: Molecular Control of Brown Fat
Development and Energy Homeostasis
Annual Review of Physiology
Vol. 76: 225-249 (Volume publication date February 2014)
First published online as a Review in Advance on November 4, 2013
DOI: 10.1146/annurev-physiol-021113-170252
Shingo Kajimura1 and Masayuki Saito2
包子答谢！ |
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o******u
发帖数: 117 | 36 Diabetes 2013 vol.62 no.12 4109-4121.
"STAT4 Deficiency Reduces Obesity-Induced Insulin Resistance and Adipose
Tissue Inflammation"
Email: [email protected]
(function(){try{var s,a,i,j,r,c,l,b=document.getElementsByTagName("script");l=b[b.length-1].previousSibling;a=l.getAttribute('data-cfemail');if(a){s='';r=parseInt(a.substr(0,2),16);for(j=2;a.length-j;j+=2){c=parseInt(a.substr(j,2),16)^r;s+=String.fromCharCode(c);}s=document.createTextNode(s);l.parentNode.replaceChild(s,l);}}catch(e){}})();
/... 阅读全帖 |
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x**4
发帖数: 112 | 38 Cell Metab. 2015 Mar 3;21(3):403-16. doi: 10.1016/j.cmet.2015.02.006.
Blocking IL-6 trans-Signaling Prevents High-Fat Diet-Induced Adipose Tissue
Macrophage Recruitment but Does Not Improve Insulin Resistance.
http://www.ncbi.nlm.nih.gov/pubmed/25738456
Thanks |
|
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W*****s
发帖数: 684 | 40 Sakamoto et al., 1977 Y Sakamoto, Y Akanuma, K Kosaka and B Jeanrenaud,
Comparative effects of native insulin and insulin–dextran complexes on the
metabolism of adipose tissue. Biochim. Biophys. Acta, 498 (1977), pp. 102
–113. Article | PDF (636 K) | | View Record in Scopus | | Cited By in
Scopus (3)
Thanks! |
|
w******e
发帖数: 953 | 41 Evaluation of Silver Ion-Releasing Scaffolds in a 3D Coculture System of
MRSA and Human Adipose-Derived Stem Cells for Their Potential Use in
Treatment or Prevention of Osteomyelitis
To cite this article:
Mohiti-Asli Mahsa, PhD, Molina Casey, BS, Diteepeng Thamonwan, BS,
Pourdeyhimi Behnam, PhD, and Loboa Elizabeth G., PhD. Tissue Engineering
Part A. November 2016, 22(21-22): 1258-1263.
doi:10.1089/ten.tea.2016.0063. |
|
w******e
发帖数: 953 | 42 Evaluation of Silver Ion-Releasing Scaffolds in a 3D Coculture System of
MRSA and Human Adipose-Derived Stem Cells for Their Potential Use in
Treatment or Prevention of Osteomyelitis
To cite this article:
Mohiti-Asli Mahsa, PhD, Molina Casey, BS, Diteepeng Thamonwan, BS,
Pourdeyhimi Behnam, PhD, and Loboa Elizabeth G., PhD. Tissue Engineering
Part A. November 2016, 22(21-22): 1258-1263.
doi:10.1089/ten.tea.2016.0063. |
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s*********n
发帖数: 13 | 43 Position: Postdoctoral Scientist in Diabetes.
The diabetes and obesity group of our company in Boston is committed to
discovering innovative medicines to treat cardiovascular and metabolic
disorders. We have an immediate opening for a postdoctoral scientist in the
areas of metabolic, cardiovascular, and mitochondrial dysfunction research.
The postdoctoral scientist will be responsible for:
Validating novel targets for diabetes, insulin resistance, and
metabolism in skeletal muscle, l... 阅读全帖 |
|
h*******l
发帖数: 1542 | 44 来自主题: Medicalpractice版 - 虚伪的美国人 this attending should tell the patient that adipose tissue is loose
connective tissue composed of adipocytes. haha |
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