l**********1
发帖数: 5204 | 1 RE LZ
Did you read that Oxford nanopare NGS any relative paper? then replied with
below sentence?
1)
Nanopore 在
测长的DNA 上面有优势，号称没有 read length 的上限
2)
nanopore不用nuclease切DNA啊，你在哪里看到的。文章里明确说了，测完后还能
recover DNA的。它这个理论上就是靠DNA不同的碱基和那个pore蛋白的物理互作测序，
可能是氢键吧
3)
but now still has assembly problem
[ 47 ]
发信人: yuem0428 (ym), 信区: Biology
标 题: Re: 测序技术的重磅炸弹--Oxford Nanopore
发信站: BBS 未名空间站 (Wed Feb 22 17:43:13 2012, 美东)
I would say there are two problems.
1. IT support. All previous sequencing data are from ... 阅读全帖 |
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b*******6
发帖数: 39 | 2 谢谢你的详细意见。首先要说，我做分子克隆的经验不是很多。其次，基本不会不改变
条件反复重复。
1 当初选用pQE8也是没有办法，因为pQE9我们实验室没有（pQE8和9的区别就是8在酶切
位点之间只有一个碱基，而9要长得多）。最开始的时候没有意识如此近的酶切位点很
难切下来，最起码同时酶切不可能切下来。但是只尝试过一次顺序酶切不行就没有再尝
试用pQE8了，我不记得是先切BamH1还是Hind3，后面尝试插一段片段在2个酶切位点之
间再切。
2 用的内切酶是Fastdigest系列，据称内切时间很短，所以我们一般都只切3个小时，
像Bamh1也不能且很长时间，因为有信号活性，最开始还以为是Bamh1星号活性导致的。
3 后来就在pQE8的Hind3位点插进去了一段几百bp的片段，构建好的克隆载体，先用
Bamh1切（因为Hind3有2个），再用Hind3切，用Bamh1切可以看到质粒线性化，再用
Hind3切可以看到切下来的片段。所以质粒酶切应该没有问题。
4 关于PCR产物酶切。当初经验不足，只在引物两端加了3bp碱基而不是4bp，但是查了
酶切位点表，3bp对各个内切酶都已经足够了... 阅读全帖 |
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n********k
发帖数: 2818 | 3 谢谢你的详细意见。首先要说，我做分子克隆的经验不是很多。其次，基本不会不改变
条件反复重复。
1 当初选用pQE8也是没有办法，因为pQE9我们实验室没有（pQE8和9的区别就是8在酶切
位点之间只有一个碱基，而9要长得多）。最开始的时候没有意识如此近的酶切位点很
难切下来，最起码同时酶切不可能切下来。但是只尝试过一次顺序酶切不行就没有再尝
试用pQE8了，我不记得是先切BamH1还是Hind3，后面尝试插一段片段在2个酶切位点之
间再切。
Nice alternative, shall solve the problem in this case...but I would just do
blunt ligation, nowadays, the fast ligation kits from different companies
such as Roche, takara, don't really care about whether it is sticky or blunt
ends, it works just as well...BTW, any one else ... 阅读全帖 |
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p****u
发帖数: 2596 | 4 we are doing 2-3bps cost per dollar trades on top 1500 stock..commition fee
is very low, and most of these 2-3bps are market impact due to large trading
volume. For some very liquid stocks or futures, the cost is below 1bps.
We can also trade passively, and even generate rebate profits in trading
arround 1bps/dollar trade, but only capture portion of the alpha.
四，五分之一都是交易费 is very good for active trading.. |
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o*****c
发帖数: 241 | 5 if you are really in a trading desk, you should know slippage+commission
cost should be less than 3bps for trading long-term index futures.
ok. let's use the nonsense 20 bps.
sharpe is 0.392 for crossing 10 sma and 0.456 for crossing 15 sma. |
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a**********2
发帖数: 355 | 6 PLEASE Note - This is not research and is not intended as such. This has
been prepared by individuals on the sales/trading desks of the Securities
Division. This material does not represent a formal or official view of
Goldman Sachs as the views expressed herein are solely those of the author(s
), which may differ from those of Global Investment Research. All prices
are indicative only. All references to “we/our” refer to the observations
of the sales and trading desk.
NY ROUNDUP – Tuesday, A... 阅读全帖 |
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y*****1
发帖数: 73 | 17 科普了。
但是查了一下貌似有好几个版本：最早的是９６年那个用3bp overhang pcr， 然后
有人用restriction sites的overhang pcr然后切vector, 再后来有人连vector都批（
一共４个ｐｃｒ）。。。请问你用的是什么ｐｒｏｔｏｃｏｌ啊 可否借鉴？还有效率
高么？ 谢谢 |
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D*a
发帖数: 6830 | 18 是啊，1 centimorgan指分离的几率是1%，约等于1000kb（不直等于是因为还是有的地
方分离几率大，有的地方几率小）
你这才3bp，能算出来大概多少人里面可以找到一个分离的。 |
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b***k
发帖数: 2673 | 19 ☆─────────────────────────────────────☆
Cyrix (The CPU) 于 (Tue Jan 1 16:16:16 2008) 提到:
有一家推荐一个idea:
To take advantage of the cheapness of the June Futures, the trader can sell
rich issues in early 2014s vusus the five- and ten- year futures or their
CTDs
所以可以这样Trade
He can sell the 4.25s of 5/2014 to buy 4.75s of 5/2012 and 4.25s of 11/2014
on a cash and duration neutral basis to pick up about 3bp in yield.
有几处不明：
1. five - and ten- year futures 的5,10是指Future expiration还是underlying
bond的 |
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