t*d
发帖数: 1290 | 1 【 以下文字转载自 Biology 讨论区 】
发信人: ttd (oldcat), 信区: Biology
标 题: better way to conquer cancer
发信站: BBS 未名空间站 (Wed Jan 16 14:57:14 2013, 美东)
IMHO, to find novel drug delivering methods might be better ways to conquer
cancer comparing to studying the mechanisms of cancer initiation and
progression.
Nature 491, S58–S60 (22 November 2012)
Nanotechnology: Carrying drugs
When Joseph DeSimone makes nanomedicines, he compares himself to a baker. He
mixes drugs with different chemical 'batters', puts them in tiny moulds,
cures them and then turns them out. He can mould almost any shape: discs,
cubes, long sticks, roughened doughnuts, or particles shaped like pollen,
viruses or red blood cells. But unlike a baker's cakes, brags DeSimone, a
chemical engineer at the University of North Carolina in Chapel Hill, every
particle in a batch will be identical, regardless of the recipe.
The materials scientists and chemists who work in nanotechnology are
creative designers, but they're also control freaks. The ability to make
particles to exact specifications, and to control their form at the
nanoscale with great precision, enables researchers to control their
function. DeSimone's various shapes can squeeze through blood vessels or
worm their way to the core of a tumour. And shape is just one of many
properties he and others can engineer at the nanoscale. Nanoparticles with
carefully controlled chemistry, size, surface charge and other properties
can carry drugs to new places and give them new functions. Nanoengineered
drug carriers can slip selectively into cancerous tissue, or protect the
drugs they carry from being destroyed before they reach their destination.
Nanomedicine has the potential to address one of the biggest problems in
cancer therapy: how to get enough of the right drug to the right place,
without causing side effects or inducing resistance. As researchers learn
more about the tumour microenvironment, and about how to engineer and
manufacture nanoparticles that carry drugs, they are gaining more control
over the course of cancer therapy — an advance that is expected to lead to
more effective treatments with fewer side effects. It is also resulting in
therapies that were previously thought impossible, such as drugs that change
their properties depending on where they are in the body, or that target
proteins once deemed undruggable. Some labs are even testing ideas inspired
by robotics and computing, such as nanoparticles that communicate with each
other to increase accumulation in a tumour.
Size matters
One of the greatest uses of nanomedicine in cancer treatments so far has
been keeping toxic drugs out of healthy tissues, says Rakesh Jain, a cancer
biologist at Massachusetts General Hospital in Boston who is also affiliated
with several drug companies. Many traditional chemotherapies are too toxic
to be given in large doses or combined with other toxic drugs. They may have
precise chemical targets but they are poor at targeting specific tissues —
they make their way blindly through healthy tissues and cancerous ones
alike, causing harmful side effects.
Doxorubicin is used to treat a range of cancers, but it can also cause life-
threatening heart damage. One of the earliest successes of nanomedicine was
Doxil, a doxorubicin-carrying nanomedicine, approved in 1995, that keeps the
drug out of the heart.
By the mid-1980s, researchers knew that 100-nanometre particles are too big
to exit healthy blood vessels but can easily escape the leaky, hastily built
vasculature that feeds tumours. Doxil was engineered to take advantage of
this. To keep doxorubicin out of the heart, researchers loaded it into a
lipid bubble about 100 nanometres in diameter. Lipids don't allow for much
engineering control, but they readily self-assemble into bubbles. When
shaken together in a water-based solution, the lipid molecules coalesce
around doxorubicin particles to create drug-delivering nanoparticles. Then,
to help the nanoparticles evade the immune system, researchers coat them
with polyethylene glycol. Once these Doxil particles accumulate in the
tumour, the drug leaks out of its carrier and attacks nearby cells.
Patients receiving Doxil have one-third the congestive heart failure
incidence of those given conventional doxorubicin, resulting in “a quantum
jump in quality of life”, Jain says. But keeping drugs out of the wrong
places is much easier than getting them into the right ones. Drugs the size
of Doxil are passively excluded from healthy tissue but cannot actively make
their way deep into a tumour, instead clustering at its perimeter. As a
result, nanomedicines offer little or no survival benefits compared with
conventional formulations, Jain says. “Now we have to improve delivery in
tumours.”
The nanomedicines currently being developed are more sophisticated than
Doxil, but many maintain the basic design of a spherical carrier with a
coating. To improve delivery, companies such as BIND Biosciences of
Cambridge, Massachusetts, are also tuning other properties such as charge,
chemistry and shape. Chief executive Scott Minick describes BIND's approach
as “medicinal nanoengineering”. Minick was previously chief executive of
Sequus Pharmecauticals, the company that developed Doxil. But unlike Doxil,
which uses simple lipids as its drug carrier, BIND's nanomedicines use
polymers, which are easier to engineer. This approach means the company can
build drug bubbles and direct where they go, control how quickly they
release a drug, and target cancer cells according to their surface markers.
The leading candidate1, BIND-014, is a 100-nanometre polymer sphere loaded
with docetaxel, a drug that kills dividing cells. Like Doxil, BIND-014
relies on its size to leave the tumour vasculature. Unlike Doxil, however,
the polymer centre has been engineered to control drug release, among other
things. The outer layer is made up of two additional components:
polyethylene glycol to help it evade the immune system, and binding
molecules that seek out markers found only on the surface of tumour cells (
see 'Nano drug carrier').
The early results of BIND-014's phase I clinical trials look promising,
Minck says. “This patient population is late-stage, terminally ill, and we
don't expect to see signs of efficacy,” he says. Even so, there are hints
that the drug is working: although the trial is still recruiting, the
company has reported that, following a course of BIND-014, tumours shrank in
two of 17 patients.
To design its therapies, BIND Biosciences tweaks the size, charge and other
properties of each part of its drug carriers, giving it control over
circulation time and drug release rate, for example. This approach allows it
to make effective nanomedicines without knowing all the biological details
of why a particle works as well as it does. Researchers elsewhere, however,
are more deliberately taking advantage of advances in understanding the
biophysics of the tumour microenvironment.
Small and squishy
Designing nanomedicines to seep out of the bloodstream into tumour blood
vessels is only the first step in cancer drug delivery2. Although a size of
100 nanometres works well for some things, it's still quite large for a drug
. “Once a big nanoparticle leaks out, it's pretty much stuck,” says
Jeffrey Hubbell, a chemical engineer at the Ecole Polytechnique Fédérale
de Lausanne in Switzerland.
Once a large particle leaves the leaky blood vessels, it has difficulty
moving deep into a tumour. Making the particle smaller would improve its
mobility, and is also an advantage when fighting certain tumours —
particularly pancreatic and some breast cancers — that are threaded with a
tough tangle of collagen, which presents a physical barrier to drugs.
But reliably making polymer nanoparticles much smaller than 100 nanometres
is tricky. Kazunori Kataoka, a materials scientist at the University of
Tokyo, Japan, developed the first polymer drug carrier in the mid-1980s. His
company, NanoCarrier, based in Kashiwa, has now developed a 30-nanometre
polymer to transport the chemotherapy drug cisplatin; it is currently
undergoing phase II clinical trials in patients with pancreatic cancer.
Cisplatin usually has severe kidney toxicity, requiring patients to drink
painfully large amounts of water during treatment. Kataoka says that's not
the case in the NanoCarrier trials because the carrier's size allows it to
move into and accumulate in the pancreatic tumour, instead of accumulating
in the kidney. “We've already successfully extended survival,” he says,
which is heartening given how difficult pancreatic cancer is to treat. In a
small phase I trial, the drug more than doubled survival time from five
months to more than twelve.
Back in North Carolina, DeSimone's work moulding strangely shaped particles
has a similar motivation: controlling a drug's size and shape to help it
enter tumours. “We want to figure out how cancer cells get in places they'
re not supposed to be, and mimic that,” he says, so that he and others can
make drugs to follow them there.
DeSimone drew inspiration for his particle-moulding method from
semiconductor manufacturing plants, which make tiny transistors by the
trillion. He can precisely vary just a single property, such as stiffness,
and then test how the particles move through the body. Using this
manufacturing method, which has been licensed by Liquidia Technologies of
Research Triangle Park, North Carolina, he can find out, for example whether
a squishier drug is better at squeezing into the centre of a tumour.
Silence will fall
One of the most promising applications for nanoengineered drug carriers is
gene silencing, in which small bits of RNA are deployed to shut down crucial
cancer genes through a process known as RNA interference. Researchers know
how to make RNA sequences that theoretically can turn off any given gene.
But without a good delivery vehicle to test the effects of these silenced
genes, finding promising therapeutic targets in animals — let alone making
an RNA therapy that will work in people — is slow going, says William Hahn,
an oncologist at Harvard Medical School in Boston, Massachusetts.
Nanocarriers may be just the technology to push this technique forward.
Designing a nanocarrier suited to transport gene-silencing RNA is tricky,
however. It must make it all the way inside a cancer cell, which requires an
escort smart enough to evade destruction. Typically, if it's not cleared
out by the liver, an RNA-carrying particle will bind to the cancer cell
membrane, which then folds inward and pinches itself off into an acidic,
destructive bubble called an endosome inside the cell.
Researchers are working on several tricks to get round this. Chemical
engineer Mark Davis at the California Institute of Technology in Pasadena
has developed a polymer carrier that absorbs positive charges as they are
pumped into the endosome. This creates an osmotic pressure that eventually
bursts the cancer cell's bubble, freeing the silencing RNA before it is
destroyed.
Traditional therapies work by binding to proteins and disabling them.
Unfortunately, most cancer genes produce proteins that are considered
undruggable by traditional means. Some of these proteins hide inside the
cancer cell, out of the reach of antibody drugs that can only get to the
surface. Other proteins have a physical shape that provides no foothold for
a drug of any kind. Nanoengineering, Davis says, could break through these
defences. With the right carrier, there is no need to go after the
undruggable proteins — gene-silencing RNA can instead go directly to the
genes that make them.
It can also target several cancer genes at once. “The goal has got to be to
hit multiple targets simultaneously” so the tumour cannot develop
resistance, says Davis. If a tumour mutates in the course of treatment,
oncologists will be able to order therapies that target those new mutations.
The logical next step
Nanotechnology researchers such as Davis, and companies like BIND, are
focused on getting more effective therapies into the clinic as quickly as
possible. But most nanomedical research has been done in vitro and in
animals; little is known about how these drugs work in people, Davis says,
although a series of clinical trials is under way (see 'Nanomedicine in
clinical trials').
Other researchers are using the tools of physical science, from robotics to
computer science, to realize more fanciful ideas about future drugs. One
prototype, made by George Church's group at Harvard University in Cambridge,
Massachusetts, is a drug-stuffed 'lock box' that opens only after
performing a simple logic operation akin to those done by computer circuits.
The box is made of DNA, a material Church and his colleagues chose for its
design possibilities. Using a technique called DNA origami, the DNA self-
assembles into a barrel shape, with locks and hinges that cause it to spring
open when particular surface markers on cancer cells — the 'keys' —
unlock them.
Church calls his DNA drug carrier a nanobot because it performs a
computational logic function: when two input signals are present (two
molecular markers on the targeted cancer cell), the box generates an output
(opening to release its drug payload). In one recent experiment3, the team
designed a cylinder of DNA that contains a cancer drug, like gems in a
jewellery box. The nanobot had two locks designed to be opened by two
proteins on the surface of aggressive leukaemia cells. The researchers
showed that the leukaemia cells could unlock the nanobot, but other cells in
the bloodstream could not.
The logic-function idea was motivated by a clinical need. Most targeted
therapies seek out just one cell surface marker. Logic-gated systems like
Church's, however, might allow for specifically targeted drugs that go after
cancer cells, such as leukaemia, but avoid healthy cells that might have a
surface marker in common with the cancer cells. But the research is still in
the early stages, as it is difficult to produce enough DNA boxes to test
the method in animal models.
Sangeeta Bhatia, a biomedical engineer at the Massachusetts Institute of
Technology, is also taking inspiration from information technology and other
fields. She is emulating natural systems and robotics to make smart
cocktails of cancer therapeutics that communicate with each other to 'swarm'
to tumours.
“Ninety percent of cancer deaths are caused by metastases,” says Bhatia.
Finding those secondary tumours is difficult, especially when they're new.
“We want to inject a therapy that will figure out where the metastasis is”
and then communicate that information to other drugs, she says, so more of
the drug reaches its target. Early demonstrations4 showed that drug-carrying
nanoparticles accumulate in a tumour in much larger numbers than they would
without such communication — in one experiment, there was a 40-fold
increase (see 'Communicating chemotherapies').
Bhatia is looking to expand this idea by incorporating design tricks from
robotics. Like ants, whose individual actions are simple but who en masse
can build a complex anthill, groups of individual robots can be programmed
to swarm and perform tasks collectively. Instituting simple rules such as “
maximize your distance from all neighbours” has allowed roboticists to make
groups of robots that fly like bees in a swarm. If Bhatia can apply this to
drugs, she might achieve even greater drug accumulation.
The work is unorthodox, but that doesn't bother Bhatia. “We want to evolve
nanomedicine away from formulations where everything is exactly the same,”
she says. Indeed, the whole field is still evolving. As researchers improve
their ability to control, manufacture and innovate at the nanoscale, they
are opening up new paths for cancer therapy. Some may prove fruitless, but
others could yield new ways to make cancer therapy less painful and more
effective. |
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