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发帖数: 21 | 1 Puma2019 发于 2020-01-29 08:58, 论坛:
标 题: 跟一个朋友聊了会，这次武汉病毒不像合成的。
quote 如下：
I performed BLASTn and Clustal alignment on the Wuhan coronavirus and cross-
annotated missing CDS regions. I found that Bat SARS-like CoVZXC21 and Bat
SARS-like CoVZC45 are the most similar coronaviruses. The sequences are
different at quite a few silent point mutations but sufficiently close (95-
93%) to indicate a lineage that has not diverged significantly- this is a
recent strain with a close common ancestor. Some regions don’t even have
silent mutations. It is very different from MERS, and sufficiently different
from SARS that it doesn’t appear to be a containment breach from the Wuhan
BSL4 lab. Also doesn’t look like a bioweapon as some suggested, there are
no telltale codon optimizations for other species, no cloning scars, no
inserted digestion sites flanking blocks, and the mutations are sporadic and
not entire blocks in motifs. If it *were* a bioweapon it would be an
ineffective one. Additionally, the nucleoprotein appears to have two
frameshifted genes interwoven in it as part of viral hyperefficient
evolution, and they are mostly conserved with a few single amino acid
substitutions.
Second, the spike protein contains an elongation in the ~250/480AA region.
This is significant since spikes are what recognize host cells and enable
infection. The MERS spike recognizes DDP4 while the SARS spike recognizes
ACE2, so mutations can cause receptor binding to vary wildly. Interestingly,
this spike appears similar to the HIV spike protein and I think HIV
neutralizing binder drugs might be efficacious- I checked the news and china
has indeed had the same idea and begun using HIV drugs as treatment.
It seems obvious to me that the virus is a bat zoonotic transmission that
arose through a random insertion in the spike protein that refolded into
human compatibility and adjusted for a different life cycle. Given the 100%
homology of the envelope proteins to the bat SARS-like viruses it is likely
this virus survives similarly outside the human body for days with a
logarithmic drop in viability typical of coronaviruses and will spread very
quickly. The snake codon paper is hot garbage. |
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