d******i 发帖数: 210 | 1 【 以下文字转载自 Engineering 讨论区,原文如下 】
发信人: doublrpi (小悯), 信区: Engineering
标 题: 恳请有工程背景的大虾指导
发信站: Unknown Space - 未名空间 (Mon Nov 3 11:22:29 2003) WWW-POST
偶是学生物的,平素就是作作细胞培养,然后取出细胞培养液用超声波破碎。可是上周老
板突发奇想,要偶设计一个连续细胞破碎装置,如下图所示,细胞液由PUMP连续输入一个
圆柱型不锈钢容器,在容器上开一孔,插入Sonication Probe,这样随着细胞液从左边流
入,流出时细胞就被打碎了。可怜偶一点工程背景多没有,PUMP, 不锈钢容器如何选,需
要什么配件,怎样安装,毫无头绪。恳请有工程背景的大虾指导。
! sonication probe
--------!-------------
----- !
| ----------------------
| vessel or tube
|
connecte | s*i 发帖数: 5025 | 2 Check
http://www.equilabcanada.com/celldisrupter.htm
see if you are trying to re-invent the wheel.
老
个
流
需
【在 d******i 的大作中提到】 : 【 以下文字转载自 Engineering 讨论区,原文如下 】 : 发信人: doublrpi (小悯), 信区: Engineering : 标 题: 恳请有工程背景的大虾指导 : 发信站: Unknown Space - 未名空间 (Mon Nov 3 11:22:29 2003) WWW-POST : 偶是学生物的,平素就是作作细胞培养,然后取出细胞培养液用超声波破碎。可是上周老 : 板突发奇想,要偶设计一个连续细胞破碎装置,如下图所示,细胞液由PUMP连续输入一个 : 圆柱型不锈钢容器,在容器上开一孔,插入Sonication Probe,这样随着细胞液从左边流 : 入,流出时细胞就被打碎了。可怜偶一点工程背景多没有,PUMP, 不锈钢容器如何选,需 : 要什么配件,怎样安装,毫无头绪。恳请有工程背景的大虾指导。 : ! sonication probe
| s*i 发帖数: 5025 | 3 It is not difficult. If you want to make a controllable device like that, what
you need are:
1. Analog output(Ni-Daq, or event use parallel port+DAC)
2. A pressure regulator
3. Compressed air tank
4. Syringe
5. Probe sonicator
周
一
边
,
【在 s*i 的大作中提到】 : Check : http://www.equilabcanada.com/celldisrupter.htm : see if you are trying to re-invent the wheel. : : 老 : 个 : 流 : 需
| j*****g 发帖数: 463 | 4 I did not see the necessary to use a pump.
Why not make your Vessel/Tube vertical?
That way, the fluid can flow by itself from top to bottom,
with a valve at the top or bottom, you can control the
flow speed. Bottom might be better, because I remember
Ultra Sonic Probe needs to submerge in the fluid to
function properly.
Why you need a stainless steel container?
I think you can just use all the glass tube and bottles
you could easily find in your lab.
If you really needs to control the flow spee
【在 d******i 的大作中提到】 : 【 以下文字转载自 Engineering 讨论区,原文如下 】 : 发信人: doublrpi (小悯), 信区: Engineering : 标 题: 恳请有工程背景的大虾指导 : 发信站: Unknown Space - 未名空间 (Mon Nov 3 11:22:29 2003) WWW-POST : 偶是学生物的,平素就是作作细胞培养,然后取出细胞培养液用超声波破碎。可是上周老 : 板突发奇想,要偶设计一个连续细胞破碎装置,如下图所示,细胞液由PUMP连续输入一个 : 圆柱型不锈钢容器,在容器上开一孔,插入Sonication Probe,这样随着细胞液从左边流 : 入,流出时细胞就被打碎了。可怜偶一点工程背景多没有,PUMP, 不锈钢容器如何选,需 : 要什么配件,怎样安装,毫无头绪。恳请有工程背景的大虾指导。 : ! sonication probe
| d******i 发帖数: 210 | 5 Thank sui, that's exaxtly what I need.
周
一
边
,
【在 s*i 的大作中提到】 : Check : http://www.equilabcanada.com/celldisrupter.htm : see if you are trying to re-invent the wheel. : : 老 : 个 : 流 : 需
| d******i 发帖数: 210 | 6 Thanks, 张老大!
周老
一个
边流
,需
【在 j*****g 的大作中提到】 : I did not see the necessary to use a pump. : Why not make your Vessel/Tube vertical? : That way, the fluid can flow by itself from top to bottom, : with a valve at the top or bottom, you can control the : flow speed. Bottom might be better, because I remember : Ultra Sonic Probe needs to submerge in the fluid to : function properly. : Why you need a stainless steel container? : I think you can just use all the glass tube and bottles : you could easily find in your lab.
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