f*********e 发帖数: 1144 | 1 Really really weird...
but it looks large precipitation happens if I dilute the buffer containing
100mM Tris.HCl(pH8) + 10mM CaCl2 with PBS (pH7.4)
Only thing I can thinki off is some sort of calcium phospate compound formed
.... is it possible???
Need help from Niu Ren |
s*******h 发帖数: 3731 | 2 ft....
formed
【在 f*********e 的大作中提到】 : Really really weird... : but it looks large precipitation happens if I dilute the buffer containing : 100mM Tris.HCl(pH8) + 10mM CaCl2 with PBS (pH7.4) : Only thing I can thinki off is some sort of calcium phospate compound formed : .... is it possible??? : Need help from Niu Ren
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l**j 发帖数: 651 | 3 终于看见个我能回答的问题了
您是学化学出身的么……?
formed
【在 f*********e 的大作中提到】 : Really really weird... : but it looks large precipitation happens if I dilute the buffer containing : 100mM Tris.HCl(pH8) + 10mM CaCl2 with PBS (pH7.4) : Only thing I can thinki off is some sort of calcium phospate compound formed : .... is it possible??? : Need help from Niu Ren
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s*******h 发帖数: 3731 | 4 晶晶,你就给我们小护士讲讲吧。。。
【在 l**j 的大作中提到】 : 终于看见个我能回答的问题了 : 您是学化学出身的么……? : : formed
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f*********e 发帖数: 1144 | 5 能回答倒是回答啊!!!!!
我问了一个同事chemist,人家说不知道pbs里面phosphate group怎么样子,无法回答
不要嘲笑阿~~~我都不知道我啥出身。。。
【在 l**j 的大作中提到】 : 终于看见个我能回答的问题了 : 您是学化学出身的么……? : : formed
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f*********e 发帖数: 1144 | 6 老大发发善心讲解一下ft得原因麻。。。
【在 s*******h 的大作中提到】 : 晶晶,你就给我们小护士讲讲吧。。。
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L*******r 发帖数: 5448 | 7 多容易验证啊,弄点CaCl2和Na3PO4放水里看看不就行了。。
formed
【在 f*********e 的大作中提到】 : Really really weird... : but it looks large precipitation happens if I dilute the buffer containing : 100mM Tris.HCl(pH8) + 10mM CaCl2 with PBS (pH7.4) : Only thing I can thinki off is some sort of calcium phospate compound formed : .... is it possible??? : Need help from Niu Ren
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f*********e 发帖数: 1144 | 8 I did, the precipitation has been confirmed(not with Na3PO4), now I am
looking for explainations if anybody could help...
【在 L*******r 的大作中提到】 : 多容易验证啊,弄点CaCl2和Na3PO4放水里看看不就行了。。 : : formed
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L*******r 发帖数: 5448 | 9 2011年8月18日 – 物竞编号:0K1J; 中文名称:三元磷酸钙; 英文名称:Calcium
phosphate ... 溶解性(mg /mL):微溶于水(溶解度0.0025g/100ml水),
【在 f*********e 的大作中提到】 : I did, the precipitation has been confirmed(not with Na3PO4), now I am : looking for explainations if anybody could help...
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