R****n
发帖数: 708 | 1 Anyone remember the rough range of total protein input? I will do a serial
dilution next week,just want to be cautious as the 1st experiment.The manual
didn't say much about this. BTW, the protein extractant is from tissue
samples.
Thanks | x**********h
发帖数: 36 | 2 我测的是pAMPK by elisa，查paper和email用过这个kit的人告诉我100ug/well.我想问
一下你是怎样从组织中提取蛋白的，我提出来的，不是失活就是量太少，检测不出来啊 | R****n
发帖数: 708 | 3 My tissue sample was homogenizd by micropestle in RIPA buffer+protease
inhibitors. The serial dilution results confirmed what you said 100ug/well,
cause my 1mg/well input showed strong signal from ERK and pERK both. I
boild my sample for 5 mins to denature proteins, not sure about your protein
. I am having some trouble in Standard curve now,but I will find out
tomorrow. |
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