e**r
发帖数: 1144 | 1 好象是histone modification.
谁知道原始的出处？ | A******y
发帖数: 2041 | 2 It should be a pretty recent paper. Lysine crotonic modification is not
known until late 2011. | f******9
发帖数: 39 | | s******s
发帖数: 13035 | 4 不会用google image search？
https://www.google.com/search?tbs=sbi:
AMhZZitkLraYJOvzih5MxI4PtlfSlTWP4LcvoIeZTEsnQaoeZugMDJwfNq4bzihBH00GMius_
1fyMHmXUeKmOrtwYgaZNAKb3fzOQH0Z9rWfJ7o7xaCRpjKXb32tNSZc9FUdufiV0A4-
QLRCtSBOSTqOyThyqxpoYdxJaa0SuX3qBCGFmVn5r0cDXuCdGdSn5M4Zb1rbbabPAdcUSqd4qBGS
9Z4ZwQ9RgtJLjXySjtiGMqZ3XkYKoxVVJ2-rGXa6_
1j8r9FgA0hYwtLIuCprjupyuXilueZv1IgtCvf3hv8ChAxWTPfA3v3-_1XxfGoRV-dnUv-Ery7UD
_
1hk17pmOGdZx5M6BEUsjaEOoGzDGZNIHMklGlXFIUoTsLcWEx26X2w7hIkdWndeT3MYlLWEZUWZV
X6-aLw12hpb4_
10hsmuV6D2Hd0YuXJy6jvohCoc4hSLraNVq1ktmxM15az0G5w0LdSGcGtQurjImdBy7VNpH4nG_
1mGKdmScijLL1mSiq0S934LPj2IXjSGMvZjWTmo1P-E4pkhJKCuqoZNiLKmZ0pICGdO6RJfB1o_
10i4bvfhmfU-vUKWeZPDBT9Ju7JtqX5hSWdUhilCCN15G_
1fKTCxXHAlPWNnlsn2qLJLEf1XYFaWBHJbZYgddJMCCNi3sOk1GETbuxS_
1ckZDUSslO3blMwAWKnn3d3ocpt3CM7QDmBD0hlfns239p20eo0vvYNKjxvQyC8M4bOPsKbIK_
1R7jpsmANpiNjzFRId0tBc46_
1JMjgIEAhXSZRxBYKO8FQJwPENN29Kz0CEBpFaFLHaDTbWOaPGvnEvsrJAR4KmwxchWsBEesypVp
z-PCeXXPEjMmRPlS50HvKxKXuT2SWocS8xWKEYOX9WkJ7_
1dLZEfeegBm1kEuzbdCBwQfyip0SX1I9FXTBcjQ41C5PF5MGeN7IYs_
1vERxJB3rlwujieYp05nHaqJpNn0VecEnI9eRXVP65gxEHvtfVYw92xJLxK6FXcxh49j1Eu3sOY0
w5mnm6tIoeHkTor8wHtXd0ovajMvv0BGq5aqPwUVirTnqGPbeAsbWBMYynbek5CTBvVnactB0iBL
-NKxbl4CRWeLwQ8XJBVKjF4RqFeHVk_
1DYj9dZ17l307w4CB5ztsL8vVqPrEGab6XTETb0z26vxuA2_
1ueBWVINm7OQrxGAUnG4V5wBzkqhvzfd4odLne2tQAHJBcMSf5ARBovtTKCz5YAKlhhx1njX7Qg7
g44t2ULQrAAo6BZdnTn8KOW0HKMesW4X0_1Nq6cmCXcsoJws47nlKgB6l46ypO7cCxpWnw&bih=
751&biw=867
【在 e**r 的大作中提到】
: 好象是histone modification.
: 谁知道原始的出处？
| S******i
发帖数: 71 | 5 据某生化大牛说, 蛋白croto似乎只是一个垃圾, 蛋白修饰很多,但是是不是生物学修饰
,还是化学性修饰,这点是修饰的关键. 据说蛋白croto只是一些化学修饰,其报道的表观
学意义是个artificial,这也是蛋白croto发表之后跟风的文章很少,应该是不能重复的
原因吧.
【在 A******y 的大作中提到】
: It should be a pretty recent paper. Lysine crotonic modification is not
: known until late 2011.
| X*********4
发帖数: 50 | 6 楼下说的对, 图里面的crotonylted是Zhao yingming他们发现的. 但是我看着很solid
的样子, 不可能是造假的噢
【在 e**r 的大作中提到】
: 好象是histone modification.
: 谁知道原始的出处？
| A******y
发帖数: 2041 | 7 It really doesn't matter if it is chemical modification or biological
modification. Some scientist in the field believe a lot of acetylation
modification is through direct chemical transfer of Acetyl-CoA without the
help of HAT espeically in organelle enviroment favoring nucleophilic
addition.
As far as crotonyl-modification. My friesd published in a good journal
stating HDAC3 is the de-crotonylase...my lab results are exactly opposite.
HDAC1 and HDAC2 have more activities against cortonyl-lysine in my hand (hey
, I didn't review that paper, don't blame me). In addition, the Kcat is
very low compare to acetylation substrate, so I don't know if it is really
biologically relevent.
【在 S******i 的大作中提到】
: 据某生化大牛说, 蛋白croto似乎只是一个垃圾, 蛋白修饰很多,但是是不是生物学修饰
: ,还是化学性修饰,这点是修饰的关键. 据说蛋白croto只是一些化学修饰,其报道的表观
: 学意义是个artificial,这也是蛋白croto发表之后跟风的文章很少,应该是不能重复的
: 原因吧.
| S******i
发帖数: 71 | 8 按照这个做法, 蛋白修饰是很多很多了, 就像蛋白解构, 每个蛋白都有解构, 也是重要
; 关键还是要有生物功能. 所以修饰的生命特征是后续研究重点, 如果following
biological significane没有文章来解析, 说明很多问题, 最大可能不太可靠的
hey
【在 A******y 的大作中提到】
: It really doesn't matter if it is chemical modification or biological
: modification. Some scientist in the field believe a lot of acetylation
: modification is through direct chemical transfer of Acetyl-CoA without the
: help of HAT espeically in organelle enviroment favoring nucleophilic
: addition.
: As far as crotonyl-modification. My friesd published in a good journal
: stating HDAC3 is the de-crotonylase...my lab results are exactly opposite.
: HDAC1 and HDAC2 have more activities against cortonyl-lysine in my hand (hey
: , I didn't review that paper, don't blame me). In addition, the Kcat is
: very low compare to acetylation substrate, so I don't know if it is really
| X*********4
发帖数: 50 | 9 估计是Zhao 实验室找到的蛋白酶? 其实你发现的和别人发现的有分歧,怎么办? 是实验
室系统的问题,
我觉得实验材料问题很大吧, 化学蛋白组实验室对蛋白样品的质量很重视,但是对生物
材料的质量和来源并不重视. 所以他们的发现能不能在其他生物系统中重复是关键, 重
复不出来的估计就是垃圾了, 至于能不能算造假,不知道.
hey
【在 A******y 的大作中提到】
: It really doesn't matter if it is chemical modification or biological
: modification. Some scientist in the field believe a lot of acetylation
: modification is through direct chemical transfer of Acetyl-CoA without the
: help of HAT espeically in organelle enviroment favoring nucleophilic
: addition.
: As far as crotonyl-modification. My friesd published in a good journal
: stating HDAC3 is the de-crotonylase...my lab results are exactly opposite.
: HDAC1 and HDAC2 have more activities against cortonyl-lysine in my hand (hey
: , I didn't review that paper, don't blame me). In addition, the Kcat is
: very low compare to acetylation substrate, so I don't know if it is really
| e**r
发帖数: 1144 | |
|
