f*c
发帖数: 2726 | 1 almost all the transfection protocols (mammalian adherent cells) say: split
cells one day before transfection. Why? why should be one day ,not two days
or more?
thanks | D****g
发帖数: 275 | 2 As I understand, it is to make sure that the cells are in best condtions and
the transcription and translational machinery are highly functional, so
your target can be highly expressed. | D****g
发帖数: 275 | 3 As I understand, it is to make sure that the cells are in best condtions and
the transcription and translational machinery are highly functional, so
your target can be highly expressed. | f*c
发帖数: 2726 | 4 I see. thanks
and
【在 D****g 的大作中提到】
: As I understand, it is to make sure that the cells are in best condtions and
: the transcription and translational machinery are highly functional, so
: your target can be highly expressed.
|
|
