m******5
发帖数: 1383 | 1 直接表达后可用的，非precursor
google半日后只找到human版本 | l**********1
发帖数: 5204 | 2 Materials and Methods
Mice
FVB/N mice carrying the human DTR transgene under control of the CD11c and
CD11b promoters obtained from Dr. R. Lang (Divisions of Ophthalmology and
Molecular and Developmental Biology, Children’s Hospital, Cincinnati, OH)
were backcrossed onto NOD/LtJ mice (origi- nally purchased from The Jackson
Laboratory) for 10–12 generations to produce CD11c-DTR/NOD and CD11b-DTR/
NOD mice;
DTR transgenes were identified by PCR on tail DNA using primer se- quences
for DTR-1 (5'-AAAGAAAGAAGAAAGGCAAGGG-3') and DTR-2 (5’-AGATGAACTTCAGGGTCAGC
-3').
PCR product size was recognized
as 509 bp on 1% agarose gel. All mice were housed and bred in the American
Association for the Accreditation of Laboratory Animal Care-approved,
specific pathogen-free facility at the Cincinnati Children’s Research
Foundation (Cincinnati, OH).
citation is from J Immunol. 179: 5041-5053.
link:
//www.ncbi.nlm.nih.gov/pubmed/17911589
【在 m******5 的大作中提到】
: 直接表达后可用的，非precursor
: google半日后只找到human版本
| m******5
发帖数: 1383 | 3 Thank you!! I have still looking for full length sequence
Jackson
AGATGAACTTCAGGGTCAGC
【在 l**********1 的大作中提到】
: Materials and Methods
: Mice
: FVB/N mice carrying the human DTR transgene under control of the CD11c and
: CD11b promoters obtained from Dr. R. Lang (Divisions of Ophthalmology and
: Molecular and Developmental Biology, Children’s Hospital, Cincinnati, OH)
: were backcrossed onto NOD/LtJ mice (origi- nally purchased from The Jackson
: Laboratory) for 10–12 generations to produce CD11c-DTR/NOD and CD11b-DTR/
: NOD mice;
: DTR transgenes were identified by PCR on tail DNA using primer se- quences
: for DTR-1 (5'-AAAGAAAGAAGAAAGGCAAGGG-3') and DTR-2 (5’-AGATGAACTTCAGGGTCAGC
| l**********1
发帖数: 5204 | 4 写伊妹儿问他要 mice DTR full sequence data
above paper last/corresponding author:
Jonathan D. Katz, PhD
Professor, UC Department of Pediatrics
Phone: 513-636-5306
Email: jonathan.katz (at) cchmc.org
from
//www.cincinnatichildrens.org/bio/k/jonathan-katz/
就说你们lab CNS submitted draft will cite his that paper bla bla bla...
【在 m******5 的大作中提到】
: Thank you!! I have still looking for full length sequence
:
: Jackson
: AGATGAACTTCAGGGTCAGC
|
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