q**********0
发帖数: 335 | 1 最近用一蛋白和p53-TA-Luc 在HepG2作共转染，发现该蛋白对p53-TA-Luc 有３倍的激
活（相对于空载体），但是，当该蛋白的ATG 被knock out后，发现该阴性对照也有激
活,还稍高一点，还稍高一点。请问这种数据怎么解释呢？谢谢！ | n********k
发帖数: 2818 | 2 not exactly sure your question or the way u described...but what I can say
is as far as my own experiences go, reporter assays are not as simple as it
sounds...more often than not, it is extremely hard to find a perfect
negative control reporter...without proper/thorough consideration, so many
factors could screw the results but give seemingly expected results...I have
seen too many performing reporter assays without a decent understanding it
but...well, they could produce nice and fast results though:))))...In short,
for the basic reporter/negative control, it is not rare that they would
show some responses...one would have to know their particular case and try
other ways to control for that or use different assays to be sure about it..
.Unlike the common practice by too many, personally I feel the key is to
disapprove a result as much as one can before accepting it...
【在 q**********0 的大作中提到】
: 最近用一蛋白和p53-TA-Luc 在HepG2作共转染，发现该蛋白对p53-TA-Luc 有３倍的激
: 活（相对于空载体），但是，当该蛋白的ATG 被knock out后，发现该阴性对照也有激
: 活,还稍高一点，还稍高一点。请问这种数据怎么解释呢？谢谢！
| s****t
发帖数: 461 | 3 make sure there is no other in-frame ATG nearby first. | q**********0
发帖数: 335 | 4 Good idea. Thanks.
【在 s****t 的大作中提到】
: make sure there is no other in-frame ATG nearby first.
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