s**u
发帖数: 9035 | 1 Molecular Biologist
Qualifications:
Advanced degree in Molecular or Cell Biology, with 5+ yrs experience or Ph.D
. with 0-2 yrs experience, in genetic engineering;
Excellent attention to detail, good communication skills;
Team oriented, but can perform independently.
The ideal candidate has recent hands-on experience in transferring antibody
genes from hybridomas to cell lines.
Principal Responsibilities:
Isolate genetic DNA from hybridomas, create vector(s) and transfect
monoclonal antibody into immune cells;
Identify commercially available antibodies of interest;
Negotiate and manage projects with antibody shops;
Reports to CSO.
Additional Information:
This position is in a dynamic company that is in the development phase of a
revolutionary biotechnology with applications in biodefense and food safety.
Contact:
Scott Perschke
PathSensors, Inc.
800 West Baltimore Street
Suite 405
Baltimore, MD 21201
www.pathsensors.com
(o) 443/557-6150
(c) 717/759-0509 | M*****n
发帖数: 16729 | 2 这个工作transferring antibody genes from hybridomas to cell lines有什么trick
吗？
.D
antibody
【在 s**u 的大作中提到】
: Molecular Biologist
: Qualifications:
: Advanced degree in Molecular or Cell Biology, with 5+ yrs experience or Ph.D
: . with 0-2 yrs experience, in genetic engineering;
: Excellent attention to detail, good communication skills;
: Team oriented, but can perform independently.
: The ideal candidate has recent hands-on experience in transferring antibody
: genes from hybridomas to cell lines.
: Principal Responsibilities:
: Isolate genetic DNA from hybridomas, create vector(s) and transfect
| c**********5
发帖数: 653 | | l******g
发帖数: 1623 | 4 如果只是把hybridoma抗体的相应gene搞出来到CHO神马的细胞里面表达，做点研究用的
材料，没啥trick。如果要大量作治疗用的抗体，学问就大了。人猿化，序列优化。。。
trick
【在 M*****n 的大作中提到】
: 这个工作transferring antibody genes from hybridomas to cell lines有什么trick
: 吗？
:
: .D
: antibody
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