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Biology版 - poly A question?
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1 (共1页)
f******9
发帖数: 22
1
Does anyone know if there is any different between human growth hormone
polyA and bovine growth hormone polyA? why some expression cassette use
human other use bovine? Any specific purpose?
Plus bovine is much short than human ones, make me wonder why we do not use
bovine poly instead.
Anyone knows? Thanks.
r***e
发帖数: 2539
2
polyA signal不是必须的,所以我觉得人的牛的区别不大。

use

【在 f******9 的大作中提到】
: Does anyone know if there is any different between human growth hormone
: polyA and bovine growth hormone polyA? why some expression cassette use
: human other use bovine? Any specific purpose?
: Plus bovine is much short than human ones, make me wonder why we do not use
: bovine poly instead.
: Anyone knows? Thanks.

m******5
发帖数: 1383
3
Reference????
And are you sure???

【在 r***e 的大作中提到】
: polyA signal不是必须的,所以我觉得人的牛的区别不大。
:
: use

m******5
发帖数: 1383
4
Oh,that is because the cases you mentioned already had polyA signal harbored
downstream.
without a validated polyA signal, the cassette you inserted would not
express at all.

【在 r***e 的大作中提到】
: polyA signal不是必须的,所以我觉得人的牛的区别不大。
:
: use

1 (共1页)
进入Biology版参与讨论
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相关话题的讨论汇总
话题: polya话题: bovine话题: poly话题: human话题: use