m******5 发帖数: 1383 | 1 5'
CTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCC
CACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGG
GTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGG 3'
I am currently designing a transgenic vector, I cloned this BGH polyA signal
from PCDNA 3.0
could anyone here familiar with it help me to check if this poly A signal is
complete enough? |
m******5 发帖数: 1383 | |
a******p 发帖数: 414 | 3 你可以到pcDNA4.0 的质粒图谱上看看,他有很详细的annotation,我当时做transgene
mice就是用的他的序列
CTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCC
CACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGG
3'
signal
is
【在 m******5 的大作中提到】 : 5' : CTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCC : CACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGG : GTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGG 3' : I am currently designing a transgenic vector, I cloned this BGH polyA signal : from PCDNA 3.0 : could anyone here familiar with it help me to check if this poly A signal is : complete enough?
|
m******5 发帖数: 1383 | 4 我是按照invitrogen PCDNA 3.0的annotation做下来的,以前吃过别的质粒的亏 (那个
质粒只把部分GT rich region标出来,turns out不够)
transgene
CTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCC
CACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGG
【在 a******p 的大作中提到】 : 你可以到pcDNA4.0 的质粒图谱上看看,他有很详细的annotation,我当时做transgene : mice就是用的他的序列 : : CTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCC : CACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGG : 3' : signal : is
|