y*********u
发帖数: 183 | 1 不能用来做chip的抗体是不是可能也不能用于western blot识别cross link过的sample
(即使在sample buffer里煮过)
刚遇到这么个问题，但想不通的地方是，cross link过的sample 在sample buffer里de
cross link了，理论上应该能识别啊 | D******9
发帖数: 2665 | 2 depends on your antibody again. crosslinking will destroy the epitope of the
protein. If you are using polyclonal Ab, you may have more chances | y*********u
发帖数: 183 | 3 but as I mentioned, the cross linking is theoretically reversed in protein
sample buffer, which means, I should be able to have it detected?
the
【在 D******9 的大作中提到】
: depends on your antibody again. crosslinking will destroy the epitope of the
: protein. If you are using polyclonal Ab, you may have more chances
| D******9
发帖数: 2665 | 4 not completely, we normally use formaldehyde to crosslink the protein.
During this process, you are also destroying the protein to some different
degree. |
|
