Does anyone know how to measure the concentration of protein that is bound
to lipid membrane? I can not measure it by UV/Vis because the phospholipid
greatly interferences the UV/Vis absorbance at very wide range.
I found a paper that used densitometry with a software (ImageQuant) to
determine the membrane bound protein concentration. Does anyone know how
reliable it would be. Many thanks!!!
l******a 发帖数: 3339
2
You can use Detergent compatible BCA assay from Biorad. Or Ellman's
reagent for thiol containing protein.
bound
phospholipid
how
【在 c*******4 的大作中提到】 : Does anyone know how to measure the concentration of protein that is bound : to lipid membrane? I can not measure it by UV/Vis because the phospholipid : greatly interferences the UV/Vis absorbance at very wide range. : I found a paper that used densitometry with a software (ImageQuant) to : determine the membrane bound protein concentration. Does anyone know how : reliable it would be. Many thanks!!!
