b*********g
发帖数: 65 | 1 因为老鼠数量现在不够，所以想用female A-Cre和male B flox/flox (A表示一种cre，
B表示要研究的gene) 来generate A-Cre+;B flox/flox的老鼠。
但是被告之，最好用male A-Cre和female B flox/flox这样的方式来交配，不然的话会
被flox site给delete掉，变成global deletion。
有谁能帮我解释一下原因吗？谢谢 |
g****e
发帖数: 137 | 2 many tissue-specific cre have distinct expression pattern between male and
female. that's why you only can setup one type of mating cage. |
n*********m
发帖数: 38 | 3 This is because there is maternal effect for some Cre lines. I used Sox2-Cre
before. The offspring from female Sox2-Cre is indistinguishable from
conventional KO ones. But if your Cre line does not have this effect, it
should be OK, I think. |
i*****n
发帖数: 53 | 4 germline activation in the female oocyte. |
