b********n
发帖数: 72 | 1 最近在做retrovirus，用过一两次，都在hood里，只带了手套，没带口罩，不知对身体
有没有危害
啊？ |
h******y
发帖数: 1374 | 2 危害很大
尽量避免
做lenti呗
【在 b********n 的大作中提到】
: 最近在做retrovirus，用过一两次，都在hood里，只带了手套，没带口罩，不知对身体
: 有没有危害
: 啊？
|
L**********O
发帖数: 1761 | 3 I have done lenti..
why is lenti better than retro?
【在 h******y 的大作中提到】
: 危害很大
: 尽量避免
: 做lenti呗
|
T**********t
发帖数: 1604 | 4 Call me ignorant... but doesn't lentivirus belong to the family of
retrovirus? |
y****i
发帖数: 2194 | 5 扯啊 lenti比retro危险多了
【在 h******y 的大作中提到】
: 危害很大
: 尽量避免
: 做lenti呗
|
b********n
发帖数: 72 | 6 做的时候是不是一定要戴两层手套，三层口罩啊？任何接触过病毒的waste是不是都要
用bleach消毒后才能go trash啊？ |
y**u
发帖数: 7459 | 7 那adnorvirus呢，和lenti比
危险主要指哪个方面？infect human? through what?
【在 y****i 的大作中提到】
: 扯啊 lenti比retro危险多了
|
j******i
发帖数: 939 | 8 Gamma-retrovirus and lenti are both efficiently transduced into cells, but
gamma-retro can not infect non-dividing cells. Both Gamma-retro and lenti
insert into genome and thus have insertional mutagenesis risk, however,
gamma-retro usually inserts into transcription start sites and more likely
to disrupt genome gene expression. Lenti virus, however, is sort of safer
virus with no preference to start point while inserting.
Adenovirus is episomal virus and can only infect dividing cells. This means
they are safer. |
y**u
发帖数: 7459 | 9 Thanks a lot! I was hesitating between adeno and lenti.
BTW do you know about semi-forest virus? in terms of efficiency and safety
issues? Thanks!
means
【在 j******i 的大作中提到】
: Gamma-retrovirus and lenti are both efficiently transduced into cells, but
: gamma-retro can not infect non-dividing cells. Both Gamma-retro and lenti
: insert into genome and thus have insertional mutagenesis risk, however,
: gamma-retro usually inserts into transcription start sites and more likely
: to disrupt genome gene expression. Lenti virus, however, is sort of safer
: virus with no preference to start point while inserting.
: Adenovirus is episomal virus and can only infect dividing cells. This means
: they are safer.
|
j****x
发帖数: 1704 | 10 Adenovirus以及基于Adenovirus的重组载体显然可以有效的感染分裂及非分裂细胞，这
也是AdV载体的一个显著优势之一。
AdV是一种相对安全的载体系统，主要原因有几个方面：
1. AdV本身是一种广泛存在的低致病性病毒，大部分人都有一定的免疫力。
2. 只感染，不整合，致癌性低。这点相比retro/lenti-viral vector自然安全性高一
些（当然，也因为不整合，不能长期稳定表达，这是相比lenti的一大劣势） 。
3. 作为疫苗和基因治疗载体已经在临床实验中广泛使用了很多年
而Lentiviral vector目前也已经发展到了第3代第4代了，实验室安全性也相当好，但
是由于基因治疗应用中必然存在的这样那样的风险，必须尽百分之一万的努力去消除哪
怕那百分之零点零一的可能性，而且之前也有多次失败的教训，所以lentivector乃至
基因治疗这个领域至今始终无法再更进一步。也许在未来载体系统的基因组定点整合能
够实现的话，整个领域能够有所突破，这个显然将会是革命性的。
anyway，实验室操作无论是retro/lenti还是AdV系统，都必须严格在BSL-2级别的环境
条件下规范操作，这样才能最大程度的保障个人的和环境的安全，也就没什么可担心的
了。
means
【在 j******i 的大作中提到】
: Gamma-retrovirus and lenti are both efficiently transduced into cells, but
: gamma-retro can not infect non-dividing cells. Both Gamma-retro and lenti
: insert into genome and thus have insertional mutagenesis risk, however,
: gamma-retro usually inserts into transcription start sites and more likely
: to disrupt genome gene expression. Lenti virus, however, is sort of safer
: virus with no preference to start point while inserting.
: Adenovirus is episomal virus and can only infect dividing cells. This means
: they are safer.
|
|
|
j******i
发帖数: 939 | 11 You got me! Adv can transduce nondividing cells for sure! A PI who I
respected most told me that Adv can not
infect non-dividing cells, so I never doubt about it!
Yes, uncertain insertional property with preference to genes is a major
limitation of using lentivirus. Although
its insertion characteristic is modified by combining with transposon and ZF
, these chimeras are less efficient
in titer and transduction compared to wide type.
Totally agree that site-directed lenti will be a great achievement to both
gene therapy and research.
【在 j****x 的大作中提到】
: Adenovirus以及基于Adenovirus的重组载体显然可以有效的感染分裂及非分裂细胞，这
: 也是AdV载体的一个显著优势之一。
: AdV是一种相对安全的载体系统，主要原因有几个方面：
: 1. AdV本身是一种广泛存在的低致病性病毒，大部分人都有一定的免疫力。
: 2. 只感染，不整合，致癌性低。这点相比retro/lenti-viral vector自然安全性高一
: 些（当然，也因为不整合，不能长期稳定表达，这是相比lenti的一大劣势） 。
: 3. 作为疫苗和基因治疗载体已经在临床实验中广泛使用了很多年
: 而Lentiviral vector目前也已经发展到了第3代第4代了，实验室安全性也相当好，但
: 是由于基因治疗应用中必然存在的这样那样的风险，必须尽百分之一万的努力去消除哪
: 怕那百分之零点零一的可能性，而且之前也有多次失败的教训，所以lentivector乃至
|
h********n
发帖数: 4079 | 12 I have the same question
【在 T**********t 的大作中提到】
: Call me ignorant... but doesn't lentivirus belong to the family of
: retrovirus?
|
j******i
发帖数: 939 | 13 Virus working should be done in class II lab. But no need to wear face mask
and actually it's quite safe if you
obey the virus working rule. Just need sometime to get used to it.
I have no experience with semi-forest virus. In most cases, if you want
your gene transiently expressed you
can choose episomal virus like Adv, in you want gene consistently expressed
you can turn to insertional virus.
Adv and lenti are well defined and commercial.
【在 b********n 的大作中提到】
: 最近在做retrovirus，用过一两次，都在hood里，只带了手套，没带口罩，不知对身体
: 有没有危害
: 啊？
|
j******i
发帖数: 939 | 14 Of course lenti belong to retrovirus family! ; ) Just because when people
say retrovirus they actually mean
gammaretrovirus. And for lenti, it is just a name to make HIV1 virus looks
not that horrible ; )
【在 h********n 的大作中提到】
: I have the same question
|
T**********t
发帖数: 1604 | 15 I C. 原来是你们的行话。让俺们外行人看了一头雾水。:D
【在 j******i 的大作中提到】
: Of course lenti belong to retrovirus family! ; ) Just because when people
: say retrovirus they actually mean
: gammaretrovirus. And for lenti, it is just a name to make HIV1 virus looks
: not that horrible ; )
|
L****S
发帖数: 76 | 16 For the retroviurs vectors, as far as I know all the commercially available
vectors are from the retrovirus that have the potential to infect human (of
course the virus but not the vectors).
But for lentivirus, there are vectors from HIV and FIV, and to my knowledge,
FIV can not infect human (even the FIV virus). So that I used FIV in my lab
and I think it is safe. Of course all virus related experiments must be
done in BSL II or higher labs (hoods). |
