b******n
发帖数: 82 | 1 http://www.cell.com/abstract/S0092-8674%2810%2901180-3
The ER UDPase ENTPD5 Promotes Protein N-Glycosylation, the Warburg Effect,
and Proliferation in the PTEN Pathway
Min Fang, Zhirong Shen, Song Huang, Liping Zhao, She Chen, Tak W. Mak,
Xiaodong Wang
* Highlights
* The ER UDPase ENTPD5 is upregulated by AKT in PTEN mutant cells
* ENTPD5 hydrolyzes UDP to UMP to promote protein N-glycosylation
* ENTPD5, along with CMPK1 and AK-1, hydrolyze ATP to AMP, increasing
aerobic glycolysis
* ENTPD5 knockdown attenuates growth factor expression and tumor growth
Summary
PI3K and PTEN lipid phosphatase control the level of cellular
phosphatidylinositol (3,4,5)-trisphosphate, an activator of AKT kinases that
promotes cell growth and survival. Mutations activating AKT are commonly
observed in human cancers. We report here that ENTPD5, an endoplasmic
reticulum (ER) enzyme, is upregulated in cell lines and primary human tumor
samples with active AKT. ENTPD5 hydrolyzes UDP to UMP to promote protein N-
glycosylation and folding in ER. Knockdown of ENTPD5 in PTEN null cells
causes ER stress and loss of growth factor receptors. ENTPD5, together with
cytidine monophosphate kinase-1 and adenylate kinase-1, constitute an ATP
hydrolysis cycle that converts ATP to AMP, resulting in a compensatory
increase in aerobic glycolysis known as the Warburg effect. The growth of
PTEN null cells is inhibited both in vitro and in mouse xenograft tumor
models. ENTPD5 is therefore an integral part of the PI3K/PTEN regulatory
loop and a potential target for anticancer therapy. | i***R
发帖数: 663 | | v*******a
发帖数: 759 | 3 还没看文章，不过"converts ATP to AMP, resulting in a compensatory
increase in aerobic glycolysis known as the Warburg effect",这个因果关系怎么
得出来的？AMPK被激活了吗？ | u*****g
发帖数: 1632 | 4 我去好好读读
Effect,
increasing
【在 b******n 的大作中提到】
: http://www.cell.com/abstract/S0092-8674%2810%2901180-3
: The ER UDPase ENTPD5 Promotes Protein N-Glycosylation, the Warburg Effect,
: and Proliferation in the PTEN Pathway
: Min Fang, Zhirong Shen, Song Huang, Liping Zhao, She Chen, Tak W. Mak,
: Xiaodong Wang
: * Highlights
: * The ER UDPase ENTPD5 is upregulated by AKT in PTEN mutant cells
: * ENTPD5 hydrolyzes UDP to UMP to promote protein N-glycosylation
: * ENTPD5, along with CMPK1 and AK-1, hydrolyze ATP to AMP, increasing
: aerobic glycolysis
| J********n
发帖数: 534 | | a****k
发帖数: 1130 | 6 一直怀疑你是我某师兄，难道真的是。。。
【在 J********n 的大作中提到】
: 见到大学隔壁宿舍的哥们了。
| w********r
发帖数: 1431 | 7 同怀疑啊。
不过我很久很久之前写信问lss的ID是不是CT，
被告知不是。。。
【在 a****k 的大作中提到】
: 一直怀疑你是我某师兄，难道真的是。。。
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