E****A
发帖数: 184 | 1 Any useful database to predict?
i tried this one
http://cubic.bioc.columbia.edu/cgi/var/nair/resonline.pl
but it seems it doesn't work very well.
Any suggestion will be appreciated.
Thanks in advance. |
b**j
发帖数: 415 | 2 putattive sequence, XXKXKXKRKR, or something about that?
you can also find some NLS seq from other protein to do an alignment
【在 E****A 的大作中提到】
: Any useful database to predict?
: i tried this one
: http://cubic.bioc.columbia.edu/cgi/var/nair/resonline.pl
: but it seems it doesn't work very well.
: Any suggestion will be appreciated.
: Thanks in advance.
|
E****A
发帖数: 184 | |
b**j
发帖数: 415 | 4 welcome
【在 E****A 的大作中提到】
: thanks for the hint
|
a***e
发帖数: 1010 | |
a***e
发帖数: 1010 | 6 http://www.expasy.org/tools/scanprosite/
SV40 NLS: PKKKRKV = B(4-5)
[rk](4,5)
Bipartite NLS: B2-X(9-12)-B(3-4)
B2-x(9-12)-B2-X-B(1-2)
[rk](2)-X(9,12)-[rk](3,4)
[rk](2)-x(9,12)-[rk](2)-X-[rk](1,2) |
t******y
发帖数: 716 | 7 我最近在做一个蛋白，有NLS，但是没有在细胞核内表达，有谁有类似的经历？ |
b**j
发帖数: 415 | 8 like p65, it has both NES and NLS, when it bind to IkBa, it locates in cyto,
but when IkBa degardated, it translocates to nuclus.
【在 t******y 的大作中提到】
: 我最近在做一个蛋白，有NLS，但是没有在细胞核内表达，有谁有类似的经历？
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