m******5 发帖数: 1383 | 1 在着手要构建一只CHIP用的转基因鼠
现在有三种标签选择
Myc
FLAG
ZZ
个人有点倾向于用ZZ标签,因为能直接亲和protein A
不知版面上是否有大佬有这方面经验的能否指点1,2,转基因鼠周期都很长,所以不敢贸然决定 |
a***e 发帖数: 1010 | 2 3xFLAG is my favorite. Sigma has the best antibody for it. |
O******e 发帖数: 4845 | 3 I love Flag antibody, too.
BTW, Flag is Sigma's patent so only they have Flag antibodies.
【在 a***e 的大作中提到】 : 3xFLAG is my favorite. Sigma has the best antibody for it.
|
m******5 发帖数: 1383 | 4 why triple flag?
how about HA? my boss love HA
【在 a***e 的大作中提到】 : 3xFLAG is my favorite. Sigma has the best antibody for it.
|
O******e 发帖数: 4845 | 5 better binding, better signals ....
【在 m******5 的大作中提到】 : why triple flag? : how about HA? my boss love HA
|