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Biology版 - How to analyze the interaction between two RNA molecules?
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1 (共1页)
q**********0
发帖数: 335
1
There are two RNAs, one is about 1800bp, one is 200bp, how to know these two
RNA molecule will complementary to each other? What kind of software we can
use? Thanks.
a***e
发帖数: 1010
2
blast?
q**********0
发帖数: 335
3
Blast is similarity, not complementary.

【在 a***e 的大作中提到】
: blast?
a***e
发帖数: 1010
o********s
发帖数: 28
5
There are two RNAs, one is about 1800bp, one is 200bp
Should they be 1800nt and 200 nt? instead of bp?
q**********0
发帖数: 335
6
Should be nt. Sorry, my mistake. Any good suggestions to analyze them?

【在 o********s 的大作中提到】
: There are two RNAs, one is about 1800bp, one is 200bp
: Should they be 1800nt and 200 nt? instead of bp?

q**********0
发帖数: 335
7
还是Blast,Blast只能寻找同源性,不能寻找互补性,没用。

【在 a***e 的大作中提到】
: http://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE_TYPE=BlastSearch&PROG_DEF=blastn&BLAST_PROG_DEF=megaBlast&SHOW_DEFAULTS=on&BLAST_SPEC=blast2seq&LINK_LOC=align2seq
h******0
发帖数: 3
8
http://www.attotron.com/cybertory/analysis/seqMassager.htm
paste RNA sequence 1 ->reverse -> complement -> blast against RNA sequence 2
X***n
发帖数: 366
9
Hybridize this two molecules to see the structure. Or link these two
sequence and then use mfold to fold the chimeric molecule.
http://dinamelt.bioinfo.rpi.edu/twostate.php
q**********0
发帖数: 335
10
Thanks a lot. Your suggestion is very helpful!

【在 X***n 的大作中提到】
: Hybridize this two molecules to see the structure. Or link these two
: sequence and then use mfold to fold the chimeric molecule.
: http://dinamelt.bioinfo.rpi.edu/twostate.php

1 (共1页)
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话题: rna话题: two话题: molecules话题: analyze话题: interaction