d***y
发帖数: 263 | 1 今天腰背太疼了，没法读下去。以下是一些大概印象, 欢迎指正。
1。理论的多，实验的少
2。原核的多，真核的少 （collins JJ 的nature paper好像有跟本错误）
3。 focus on txn 的多，post-txn 的少
4。绝对重要，很多critical gene protein level is very low, understand how noise
is generated or filtered out is very important.
5.实验室有一些ground work,能节省不少时间
conclusion: Seem like worth pursuing a career on this...??? | d***y
发帖数: 263 | 2 3 ideas:
1. Analyze noise in siganling cascade and see how cells deal with it
2. screen for noisy genes using the constructs we have and pick up some to
analyze it
3. Analyze noise at the level of RNA decay, translation. Because most of the
work focus on txn level of noise. But I can't vision the significance very
well as txn is indeed the most important step in noise control.
???
is | c**y
发帖数: 127 | 3 As you know, is there any good technique to monitor fluctuations of
level of RNA decay, translation on single cell levels in real time?
It's much easier to monitor the production of proteins by ?FP.
There is a new article on Nature genetics:
Vol 36, No. 2. 2004
【在 d***y 的大作中提到】
: 3 ideas:
: 1. Analyze noise in siganling cascade and see how cells deal with it
: 2. screen for noisy genes using the constructs we have and pick up some to
: analyze it
: 3. Analyze noise at the level of RNA decay, translation. Because most of the
: work focus on txn level of noise. But I can't vision the significance very
: well as txn is indeed the most important step in noise control.
: ???
:
: is
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