Re: 细胞长得太密会不会影响transfected protein的表达 - Biology版

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Biology版 - Re: 细胞长得太密会不会影响transfected protein的表达

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话题: cells话题: require

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c*****t
发帖数: 1084

check the protocol of agent. some agents require highly confluent
cells to perform transfection. some require 50%. generally
overgrown cells are not good for transfection.

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相关主题
● which mice or rat cells are easy to transfect?	● mammalian cells transfection 后 integration 的效率大概是多少？
● transfection and cell cycle synchronization	● Arguing with Boss with co-transfection
● need help on Macrophage RAW 264.7 cells	● MLE-15 transfection
● Question: If I wanna perform flow-cytometry to check function of a gene...	● 在293T细胞中做CoIP，细胞最多可以培养几天？
● 求助：大家有没有遇到过这样的问题？	● 向大家请教transfection的问题
● 请教有人用reagent transfect protein吗？	● About establishing stable transfected cell line
● 疑似关键： guides can only be loaded when NgAgo protein is in the process of expression	● 有国内的药厂要我在美国为他们卖G418，Geneticin
● Re: How long does it take for expression	● 向还在实验室的同学们问好，顺便问个包装lentivirus的问题

相关话题的讨论汇总
话题: cells话题: require

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