t***u
发帖数: 230 | 1 You don't need 5' phosphorylated primers if you are only going to use one set
of complementary primers. If you are using multiple primers to introduce
multiple changes simultaneously, then you need to phosphorylate the 5'.
Please refer to the details in their manuals:
http://www.stratagene.com/manuals/200518.pdf
Here are a few personal comments on the Stratagene's Quickchange system:
1. NO NEED to buy their kit, it's very over-priced. You need to get a high
fidelity DNA polymerase (for example, |
|
