g***m
发帖数: 465 | 1 hehe, it is a basic strategy via accurate MS for proteomic level's screening
and identifying.
The precision is really dependant on how you construct the peptide map
database. Generally, they take cDNA translated amino acid sequence as actual
proteins, then do a in silica proteolysis with trypsin, input the results into
the database.
This approach is really database dominating. Not mention possible
post-translational modification and signal peptides and so on that could bring
a big trouble. |
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