l*****k
发帖数: 587 | 1 I have some experience in this, not so good experiences:
local hopping using P element depends a LOT on the specific P element you
are dealing with, some hop well, some never hop. I spent more than half a year
trying to hop one at one point, never give me deletion in the gene,(only 52
bp away),I have to use EMS to get my mutant later.
another guy was lucky, he hopped several times and got a deletion (in another
gene). So, if you can't make it work after a while, try something different.
One thin |
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