b******n
发帖数: 4225 | 1 1. 抗体的选择
对于国内的大多数实验室来讲，做western blot实验选择抗体是个头疼的问题。原因很
简单，买进口抗体捉襟见肘，买国产抗体得需要大无畏的勇气，对于我所在的兰州地区
的实验者而言，感触尤深。在 这五年的western blot实验历程里，我先后用过进口抗
体，进口抗体国内分装包装，国产抗体，质量良莠不齐。
进口抗体一般不会出现闪失：abcam品种全，质量过硬，但价高（3400元/100微升），
而且说是100微升，但至多能吸出来90微升；Sigma的 价最贵；比较有性价比的是CST的
抗体，现在好像是2200元/100微升，我前后用过二十几种， 1:1000的稀释比下，还没
有失过手，100微升通常能完成所有的免疫组化和western blot实验，还有一个优点是
通常量比100微升多出来10微升，唯一的不足是CST的品种实在不多。Santa的多克隆抗
体质量可以，但是选用 Santa的单抗还是有风险，估计这也是业界共识了吧。
进口抗体国内分装包装我也用过不少，呵呵，毕竟是穷人（420元/100微升），大概好
抗体的比例约为50%，如果能做出来，也存在一个问题，就是... 阅读全帖 |
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i****g
发帖数: 3896 | 2 写得比较有意思，但是不太实用，可能实验条件相差太大。precast gel比自己制的gel
效果要好很多，结果也容易重复。antibody要看运气的，呵呵。
1. 抗体的选择
对于国内的大多数实验室来讲，做western blot实验选择抗体是个头疼的问题。原因很
简单，买进口抗体捉襟见肘，买国产抗体得需要大无畏的勇气，对于我所在的兰州地区
的实验者而言，感触尤深。在 这五年的western blot实验历程里，我先后用过进口抗
体，进口抗体国内分装包装，国产抗体，质量良莠不齐。
进口抗体一般不会出现闪失：abcam品种全，质量过硬，但价高（3400元/100微升），
而且说是100微升，但至多能吸出来90微升；Sigma的 价最贵；比较有性价比的是CST的
抗体，现在好像是2200元/100微升，我前后用过二十几种， 1:1000的稀释比下，还没
有失过手，100微升通常能完成所有的免疫组化和western blot实验，还有一个优点是
通常量比100微升多出来10微升，唯一的不足是CST的品种实在不多。Santa的多克隆抗
体质量可以，但是选用 Santa的单抗还是有风险，估计这... 阅读全帖 |
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w********h
发帖数: 12367 | 3 【 以下文字转载自 Biology 讨论区 】
发信人: bulletin (bulletin), 信区: Biology
标 题: 穷人的劳斯莱斯——五年western经验谈（转）
发信站: BBS 未名空间站 (Fri Mar 2 23:29:05 2012, 美东)
1. 抗体的选择
对于国内的大多数实验室来讲，做western blot实验选择抗体是个头疼的问题。原因很
简单，买进口抗体捉襟见肘，买国产抗体得需要大无畏的勇气，对于我所在的兰州地区
的实验者而言，感触尤深。在 这五年的western blot实验历程里，我先后用过进口抗
体，进口抗体国内分装包装，国产抗体，质量良莠不齐。
进口抗体一般不会出现闪失：abcam品种全，质量过硬，但价高（3400元/100微升），
而且说是100微升，但至多能吸出来90微升；Sigma的 价最贵；比较有性价比的是CST的
抗体，现在好像是2200元/100微升，我前后用过二十几种， 1:1000的稀释比下，还没
有失过手，100微升通常能完成所有的免疫组化和western blot实验，还有一个优点是
通常量比100微升多出来10... 阅读全帖 |
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c****x
发帖数: 6601 | 4 Stephan Vansonderen
http://www.quora.com/Why-is-it-easy-to-find-Westerners-obsessed-with-Japan-and-Korea-but-not-China
Do you want a real answer or a psyop? The real reason why westerners are "
obsesed" with Japan, and SOUTH Korea is because Japan, and south Korea are
obediant to the west. Western oligarks want to hold south Korea, and Japan
up to a pedestal, because they want to set them as an example. Kind of like
the model minority. You play by the rules, and we’ll give you recognition.
Certa... 阅读全帖 |
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L***O
发帖数: 2571 | 5 Stay 2 separate times at any Best Western betw. 9/12 and 11/21/10 earn
double points
Stay 2 separate times at any Best Western betw. 9/12 and 11/21/10 earn
double points
Stay 2 separate times betw. 9/12 and 11/21/10 earn double points - (Sep 12,
2010 - Nov 22, 2010) - Promo Code: FALL10 - Best Western Rewards members (
Members) residing in any participating country who stay* two (2) separate
times between September 12, 2010 and November 21, 2010 (Promotion Period) at
any Best Western branded hot... 阅读全帖 |
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yf
发帖数: 272 | 6 http://jop.ascopubs.org/content/early/2013/03/26/JOP.2012.00081
Perspective
Practicing Western Oncology in Shanghai, China: One
Group’s Experience
By David H. Garfield, MD, Harold Brenner, MD, FRCR (Lond), and Lucy Lu, RN
ProMed Cancer Centers–Shanghai, Shanghai, People’s Republic of China
In October 2011, we—David H. Garfield, MD, medical oncologist,
United States; Harold Jacob Brenner, MD, FRCR,
radiation oncologist, Israel; and Lucy Lu, oncology nurse,
Shanghai, China—were part of a group tha... 阅读全帖 |
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S****6
发帖数: 214 | 7 为学区和进程上班方便，可以考虑到 Western Springs 或La Grange 落脚吗？
收入不高，没法考虑到 Hinsdale 买房。 Hinsdale 租房子又担心孩子与同学攀比有压
力。
大家喜欢的 Naperville, 担心上班远些。
怎么感觉 Western Springs,, La Grange 有些地方小学和高中也不错，就是亚裔极少
，几乎都白人。大家有在那片居住的吗？能否提供些信息？
About Western Springs
Western Springs has 5 schools. One of the best schools is Field Park
Elementary School, which has a rating of 10. Field Park Elementary School
has 304 children. Public schools in Western Springs are part of Western
Springs School District 101. Lagrange Highlands School D... 阅读全帖 |
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y*******4
发帖数: 3837 | 8 二手交易风险自负！请自行验证是否合法和一手卡！:
我想卖的物品:
$50 Best Western Travel Card
单张面值:
$50
可接受价格(必须明码标价！):
u offer the price
物品新旧要求:
new
邮寄方式要求:
free code or u choose u pay for the physical card
买卖双方谁承担邮寄损失(Required if not code only):
b4 delivery me, after u
付款方式说明:
boa
non-cc paypal for old ID
其他补充说明:
$50 Best Western Travel Card is good at any BEST WESTERN, BEST WESTERN PLUS,
or BEST WESTERN PREMIER hotel through November 1, 2012.
广告的有效期:
till gone
物品来源(Required for All Cards!):
from Best Western rewards
我的联系方式:... 阅读全帖 |
|
y*******4
发帖数: 3837 | 9 二手交易风险自负！请自行验证是否合法和一手卡！:
我想卖的物品:
$50 Best Western Travel Card
单张面值:
$50
可接受价格(必须明码标价！):
ur best offer
物品新旧要求:
new
邮寄方式要求:
free code or u choose u pay for the physical card
买卖双方谁承担邮寄损失(Required if not code only):
b4 delivery me, after u
付款方式说明:
boa
non-cc paypal for old ID
其他补充说明:
$50 Best Western Travel Card is good at any BEST WESTERN, BEST WESTERN PLUS,
or BEST WESTERN PREMIER hotel through November 1, 2012.
广告的有效期:
till gone
物品来源(Required for All Cards!):
from Best Western rewards
我的联系方式:
bbs... 阅读全帖 |
|
y*******4
发帖数: 3837 | 10 二手交易风险自负！请自行验证是否合法和一手卡！:
我想卖的物品:
$50 Best Western Travel Card
单张面值:
$50
可接受价格(必须明码标价！):
ur best offer
物品新旧要求:
new
邮寄方式要求:
free code or u choose u pay for the physical card
买卖双方谁承担邮寄损失(Required if not code only):
b4 delivery me, after u
付款方式说明:
boa
non-cc paypal for old ID
其他补充说明:
$50 Best Western Travel Card is good at any BEST WESTERN, BEST WESTERN PLUS,
or BEST WESTERN PREMIER hotel through November 1, 2012.
广告的有效期:
till gone
物品来源(Required for All Cards!):
from Best Western rewards
我的联系方式:
bbs... 阅读全帖 |
|
h******t
发帖数: 478 | 11 我有两张Best Western HOTEL 的Free Night Voucher 可以在美国或加拿大任意一家Best Western HOTEL 住宿
一般在Best Western 住一晚包税要$100左右
我想以每张$50的价格转让，也就说是您在美国或加拿大任意一家Best Western HOTEL住宿一晚只需$50无需另外缴税。
这两张voucher 的过期日期是 December 25, 2010
您可以在同一旅馆连续住两晚或在不同的Best Western 各住一晚。打电话预约旅馆时，只要告诉对方您将用Best Western Free Night Voucher 结帐即可。
有意者可站内联系，谢谢. |
|
y*******4
发帖数: 3837 | 12 二手交易风险自负！请自行验证是否合法和一手卡！:
我想卖的物品:
$50 Best Western Travel Card
单张面值:
$50
可接受价格(必须明码标价！):
offer the price
物品新旧要求:
new
邮寄方式要求:
free code
买卖双方谁承担邮寄损失(Required if not code only):
b4 delivery me, after u
付款方式说明:
boa
non-cc paypal for old ID
其他补充说明:
$50 Best Western Travel Card is good at any BEST WESTERN, BEST WESTERN PLUS,
or BEST WESTERN PREMIER hotel through November 1, 2012.
广告的有效期:
till gone
物品来源(Required for All Cards!):
from Best Western rewards
我的联系方式:
bbs mail
Warranty期限:
能否证明是合法的一手卡?(Require... 阅读全帖 |
|
y*******4
发帖数: 3837 | 13 二手交易风险自负！请自行验证是否合法和一手卡！:
我想卖的物品:
$50 Best Western Travel Card
单张面值:
$50
可接受价格(必须明码标价！):
ur best offer
物品新旧要求:
new
邮寄方式要求:
free code or u choose u pay for the physical card
买卖双方谁承担邮寄损失(Required if not code only):
b4 delivery me, after u
付款方式说明:
boa
non-cc paypal for old ID
其他补充说明:
$50 Best Western Travel Card is good at any BEST WESTERN, BEST WESTERN PLUS,
or BEST WESTERN PREMIER hotel through November 1, 2012.
广告的有效期:
till gone
物品来源(Required for All Cards!):
from Best Western rewards
我的联系方式:
bbs... 阅读全帖 |
|
h******t
发帖数: 478 | 14 我有两张Best Western HOTEL 的Free Night Voucher 可以在美国或加拿大任意一家Best Western HOTEL 住宿
一般在Best Western 住一晚包税要$100左右
我想以每张$50的价格转让，也就说是您在美国或加拿大任意一家Best Western HOTEL住宿一晚只需$50无需另外缴税。
这两张voucher 的过期日期是 December 25, 2010
您可以在同一旅馆连续住两晚或在不同的Best Western 各住一晚。打电话预约旅馆时，只要告诉对方您将用Best Western Free Night Voucher 结帐即可。
有意者可站内联系，谢谢. |
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p*********w
发帖数: 606 | 16 我现在微博上也倡议这个词了"western sour grapes"。叶诗文被质疑这个事要搞大,现
在西媒已经开始分化,英国卫报办了个投票调查要不要让最早提出质疑的美国教练John
Leonard道歉，狗咬狗最好。
nytimes那篇质疑的文章我想在comment里加这个倡议，被五美分们删了。
大家看有没有能加上去的。檄文如下：
NYTimes published two articles relating Ye's gold medals with doping, even a
fter she passed the drug test. Obviously NYTimes, as well as some other west
ern medias, are just proving eagerly to the world of its fearing and jealous
to a rising China, again. I propose to use and spread the phrase "western s
our grapes" to refer to thi... 阅读全帖 |
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h******t
发帖数: 478 | 17 我想卖的物品:
Best Western HOTEL Free Night Voucher x 2
单张面值:
一般在Best Western 住一晚包税$100左右
可接受价格(必须明码标价！):
$50x2
物品新旧要求:
new in hand
邮寄方式要求:
you choose you pay
买卖双方谁承担邮寄损失(Required if not code only):
Before you After me
付款方式说明:
boa
其他补充说明:
Valid through December 25, 2010
Valid for two free room night at any Best Western hotel in the U.S. or Canada
您可以在同一旅馆连续住两晚或在不同的Best Western 各住一晚。打电话预约旅馆时，只要告诉对方您将用Best Western Free Night Voucher 结帐即可。
广告的有效期:
until gone
物品来源(Required for All Cards!):
bestwestern p... 阅读全帖 |
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a********b
发帖数: 1357 | 18 看了版上大家关于如何往国内汇钱的经验贴,我上周五去附近的一家western union 往
国内汇18,000
USD. 手续费26 刀. 没想到这是僵梦的开始.
我在western union 耗了2个小时, 都没有把钱成功汇出去. 首先是我的bank 不prove,
然后
western union 又来电话问我钱哪里来的? 要汇去中国干什么. 我说是家务事. 他们也
一定追根抛
低问是什么家务事. 我最后只好说是买房. 然后他们让我在电话上等了10 分多钟,说要
我提供国内的买
房证明, 否则就不给汇. 我说那就cancel order.
然后更加麻烦的是, 我折腾了3天了, 也没把我的钱从western union 拿回来. 现在还
在奔波. 总
之很麻烦, 很崩溃.
劝大家如果要寄1万美金以上, 还是要走银行. western union 狗屁都不是. |
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s*****w
发帖数: 185 | 19 【 以下文字转载自 TopArticle 讨论区 】
发信人: SpringIsGone (DogFish), 信区: Biology
标 题: 大家老板有多少要求把western结果定量做统计分析的？
发信站: BBS 未名空间站 (Fri Jul 8 13:04:43 2011, 美东)
刚完成一个项目，数据已经弄好了，文章也写得差不多了。结果老板说要把每个
western band定量，然后再把重复的2遍结果也定量，计算平均值，做统计分析。看看p
value
我简直是要吐血。快50个western结果，我都重复了3次，只挑了最好的扫描。
现在要把他们都重新扫描，定量，做统计分析，简直是有病。
你跟他说大部分文章都不定量的，即使定量也没有必要算平均值。他就说有的文章就这
样干了。
让他这么一搞，工作量不知道增加多少，关键是没有意义，纯粹浪费时间。
我们是个定量实验室，什么结果都要定量，免疫荧光看看位置的也要定量。
大家老板有这么固执的吗？根本不听劝，实验室的人都认为没必要，就他一个人坚持。
你在别的实验室做3个项目的时间，在这实验室只能做1个。
还有一个有意思的事。
他说我很多... 阅读全帖 |
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s*****g
发帖数: 7857 | 20 以前做过植物蛋白提取.是要在提取液里(研钵中)反复研磨的.过滤,然后再...
另外,现在,普通ECL难以检测到的蛋白,可以用Millipore的
Immobilon Western Chemiluminescent Substrate
500mL Cat No. WBKLS0500
你可以买小包装.另外这个试剂背景较严重,(但对应相应分子量的Marker,你可以找到你
的相应蛋白带)他们最近出了消背景的试剂.我没有用过消背景的试剂.
通常我是把普同ECL和Millipore的Immobilon Western Chemiluminescent Substrate对
半混用或(普同ECL多些,而Immobilon Western Chemiluminescent Substrate少些)
往往普同ECL结果空白一片,洗洗膜,用普同ECL和Millipore的Immobilon Western
Chemiluminescent Substrate对半混用(或普同ECL多些,而Immobilon Western
Chemiluminescent Substrate少些),30秒看初步结果,然 |
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l*****7
发帖数: 8463 | 22 Western media's shamelessly racial discrimination
Negative drug tests cannot prove no doping !!!!!!!!!!!
This is the argument which has been frequently used and accepted in the
Western media.
Based on this argument, the western media attack any athletes they
discriminate.
I agree with this argument in that you need to suspect the people who have
the ability to do things or performed in an extra-ordinary manner.
However, there is a problem here. To the common people, all the Olympic
athletes are... 阅读全帖 |
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r*****s
发帖数: 184 | 23 Official in China Says Western-Style Democracy Won’t Take Root
A Chinese legislative official has said that China will not adopt Western-
style democracy, marking a rare instance in which a member of the government
here openly rejects Western-style liberal political reforms
The official, Li Fei, said in an interview published Saturday on the Web
site of China Daily that “different countries have different election rules
and a socialist China won’t follow Western election campaigns.”
Mr. Li is d |
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l*****7
发帖数: 8463 | 24 Western media's shamelessly racial discrimination
Negative drug tests cannot prove no doping !!!!!!!!!!!
This is the argument which has been frequently used and accepted in the
Western media.
Based on this argument, the western media attack any athletes they
discriminate.
I agree with this argument in that you need to suspect the people who have
the ability to do things or performed in an extra-ordinary manner.
However, there is a problem here. To the common people, all the Olympic
athletes are... 阅读全帖 |
|
l*****7
发帖数: 8463 | 25 One way to counter them is whenever a western athlete won a medal, we say
doping, too.Because there is no other better way to counter their attack.
Well, this world is a racial world if we all do the things according to the
standard WESTERN MEIDA WAY.
The western media also promote many types of bias, discrimination and
threats, with the characteristics of mafia mentality. You can elaborate
more on these if you just pay a little attention when reading...
Therefore, the western media is a tota... 阅读全帖 |
|
l*****7
发帖数: 8463 | 26 Western media's shamelessly racial discrimination
Negative drug tests cannot prove no doping !!!!!!!!!!!
This is the argument which has been frequently used and accepted in the
Western media.
Based on this argument, the western media attack any athletes they
discriminate.
I agree with this argument in that you need to suspect the people who have
the ability to do things or performed in an extra-ordinary manner.
However, there is a problem here. To the common people, all the Olympic
athletes are... 阅读全帖 |
|
l*****7
发帖数: 8463 | 27 One way to counter them is whenever a western athlete won a medal, we say
doping, too.Because there is no other better way to counter their attack.
Well, this world is a racial world if we all do the things according to the
standard WESTERN MEIDA WAY.
The western media also promote many types of bias, discrimination and
threats, with the characteristics of mafia mentality. You can elaborate
more on these if you just pay a little attention when reading...
Therefore, the western media is a tota... 阅读全帖 |
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s****y
发帖数: 704 | 28 http://elections.foxnews.com/2008/10/15/congressman-says-western-pa-racist-obama-win/#
Congressman Says Western Pa is Racist But Obama Should Win
U.S. Rep. John Murtha said his home base of Western Pennsylvania could
reduce Obama's margin of victory by 4 percentage points
AP
Wednesday, October 15, 2008
WASHINGTON -- Democratic Rep. John Murtha said Wednesday his home base of
western Pennsylvania is racist and that could reduce Barack Obama's victory
margin in the state by 4 percentage points.
Th |
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x******x
发帖数: 1903 | 31 想卖的物品:
Best Western $25 Travel Card @ $15
Expire 6/30
This $25 Best Western Travel Card® good at any Best Western®
branded hotel through June 30, 2015. To redeem, just give the Travel Card
number below to the front desk at check-in.
Unable to be used on any full pre-paid rates
The Travel Card is a stored value card and is fully transferable.
单张面值:
$25
1 available
可接受价格(必须明码标价！):
$15
物品新旧要求:
邮寄方式要求:
Email code
买卖双方谁承担邮寄损失(Required if not code only):
N/A
付款方式说明:
paypal or BOA
我的联系方式:
PM ... 阅读全帖 |
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x******x
发帖数: 1903 | 35 想卖的物品:
Best Western $25 Travel Card @ $15
Expire 6/30
This $25 Best Western Travel Card® good at any Best Western®
branded hotel through June 30, 2015. To redeem, just give the Travel Card
number to the front desk at check-in.
Unable to be used on any full pre-paid rates
The Travel Card is a stored value card and is fully transferable.
单张面值:
$25
1 available
可接受价格(必须明码标价！):
$15
物品新旧要求:
邮寄方式要求:
Email code
买卖双方谁承担邮寄损失(Required if not code only):
N/A
付款方式说明:
paypal or BOA
我的联系方式:
PM or Ema... 阅读全帖 |
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p******x
发帖数: 232 | 36 准备圣诞期间去Cruise,初步计划从new oeleans出发走Western Caribbean一线，以下
行程看不出区别，有没有去过的人给点建议哈。欢迎有感兴趣的驴友一起。
1. 7 Night Western Caribbean
Navigator of the Seas
Ports of Call:
Day 1: New Orleans, Louisiana
Day 2: At Sea
Day 3: At Sea
Day 4: Falmouth, Jamaica
Day 5: George Town, Grand Cayman
Day 6: Cozumel, Mexico
Day 7: At Sea
Day 8: New Orleans, Louisiana
2. 7 Night Western Caribbean
Norwegian Star
Ports of Call:
Day 1: New Orleans, Louisiana
Day 2: At Sea
Day 3: Costa Maya (Mahahual), Mexic... 阅读全帖 |
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n*********n
发帖数: 5605 | 37 卖掉个镜头,病人要用Western Union付钱
问我要名字信息.
第一次遇到这个事儿
病人feedback: 51, 100%.
怎么办？
====================== Original Message ===============================
Hello! Thank you for accepting my offer. Please help me to pay for it (no, I
don't need credit - haha). I am in Europe right now and for some reason
paypal won't accept my European VISA card and my U.S. Visa card has too
little funds on it. I can send you the money immediately by Western Union or
by bank transfer (the first being much faster, of course, ... 阅读全帖 |
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d*********e
发帖数: 3835 | 38 Western-built aircraft with more than 100 seats make up 74% of the Russian
airline fleet and 28% in the regional fleet.
Speaking at last week’s Aircraft Maintenance Russia and the CIS conference
in Moscow, Vasiliy Shapkin, head of State Research and Development Institute
for Civil Aeronautics, said that in 2011 89% of Russia’s passengers flew
in Western-built aircraft. Passenger traffic on Russian aircraft decreased
from 9% in 2006, the largest in history, to 5% in 2011. Soviet-built
aircraft ha... 阅读全帖 |
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a***a
发帖数: 12425 | 39 对不住各位，这种鸟实在忒闲不住了，刚想近身就飞了，所以我压根儿还没拍到能清楚
数毛的。可是心里实在痒痒，想知道是什么鸟。你们看看能看得出来么？如果实在模糊
没关系，我以后争取照到清晰的。
又，在维基的List of birds of California上我查bluebird，有 Western Bluebird和
Mountain Bluebird两种，但是看图都不太像。
全景图（有两只小蓝鸟）
不清晰放大图
又，在回收站找到一张可以体现嘴的颜色的，这是最大的截图，再放大就眼花了该。凑
活瞧瞧吧。
===============================================
感谢李冰冰和小五，这种鸟叫Western Scrub Jay。唯一一点点区别就是，我那些不清
晰的鸟好像是橘红嘴，网上的Western Scrub Jay都
是灰黑嘴。也许我的那个是光线关系，以后我得留心，争取能早日照到清晰的。
以下为网图，挺漂亮的这鸟。
http://2.bp.blogspot.com/-wGYmgTcR9GE/TciEzIQzf8I/AAAAAAAARpU/3pGNMYfj... 阅读全帖 |
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w*******y
发帖数: 60932 | 40 WD TV HD Media Player WDAVN00B MKV H.264 ISO USB 2.0 1080p with HDMI.
Factory Recertified with 6 Month direct warranty with WD. WDAVN00BN
WDAVP00BE WDAVN00BS WDAVP00BS. Player and its contents will be ship in a
brown or white box as Factory Recertified.
Kit ContentsWD TV HD Media Player
Compact remote batteries not included
Media Converter software Can Be Downloaded Here
Composite AV cable
AC Adapter
Media File Format Support (Based on Firmware Version 1.01.01)Movie -- MPEG1/
2/4, WMV9, AVI (MP... 阅读全帖 |
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w*******y
发帖数: 60932 | 41 Target (In store only) has the Western Digital My Passport 750GB portable
hard drive (USB powered) for $59.99.
Linky:
http://www.target.com/Western-Digital-Passport-Essential-Portab Digital Passport Essential Portable&node=366491011&searchSize=30&searchView=list&searchPage=1&sr=1-9&qid=1309092336&rh=&searchBinNameList=target_com_category-bin,target_com_brand-bin,price&searchRank=salesrank&frombrowse=1
for specs.
Also in-store is the 1.5TB WD external (non-USB powered) drive for $59.99.
Linky:
... 阅读全帖 |
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w*******y
发帖数: 60932 | 44 YMMV
I just came home from shopping at Costco in Sterling, VA, where they were
selling Western Digital 3TB USB 3.0 external HDDs for $109.99 after INSTANT
rebate (limit 2), as seen in my very blurry attached camera phone picture (
My apologies, it was a very busy Saturday afternoon). My picture is cropped,
but they had a lot in stock (25+).
Costco.com only has it for $129.99 + $10 shipping.
This is my first deal, so please pardon any mistakes. for looking!
Other prices:
Link:
http://slickdeals.... 阅读全帖 |
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h********a
发帖数: 1120 | 45 http://www.returnofkings.com/53410/the-decimation-of-western-wo
A new song by rising Swedish pop star Tove Lo serves as a reminder to
Western men how broken the current generation of women have become. Have a
watch at this video:
The song has a simple message: a young woman gets hurt by a guy and decides
to ease the pain by going to the club, getting drunk, smoking reefer, and
fucking random guys. This coping strategy doesn’t work too well in the
video—a brief but visceral moment in the end show... 阅读全帖 |
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c*****a
发帖数: 8464 | 46 二手交易风险自负！请自行验证是否合法和一手卡！:
Y
我想卖的物品:
住Best Western hotel折扣
单张面值:
$5. For any hotels at Best Western。
可接受价格(必须明码标价！):
你在Best Western的hotel住一次，我给你$5 cash。
可以用gc，可以用coupon，可以走任何方式check out，甚至在他家前台定都可以。
不用我帮你定，只要帮我攒stay就可以。
物品新旧要求:
邮寄方式要求:
买卖双方谁承担邮寄损失(Required if not code only):
付款方式说明:
其他补充说明:
广告的有效期:
物品来源(Required for All Cards!):
我的联系方式:
Warranty期限:
能否证明是合法的一手卡?(Required for All Cards!):
state and zip: |
|
s*****w
发帖数: 185 | 47 【 以下文字转载自 Joke 讨论区 】
发信人: dumpty (humpty), 信区: Joke
标 题: 唐骏澄清说博士学位是从Pacific Western University拿的 (转载)
发信站: BBS 未名空间站 (Tue Jul 6 01:51:09 2010, 美东)
发信人: iceui (iceui), 信区: SanFrancisco
标 题: 唐骏澄清说博士学位是从Pacific Western University拿的
发信站: BBS 未名空间站 (Tue Jul 6 00:57:29 2010, 美东)
看明白了，他是用这种方法蒙混别人的：在加州理工学习过，有博士学位。
Pacific Western就是又一个克莱登。
http://tech.sina.com.cn/i/2010-07-06/11424391745.shtml
“我的整体的经历是，在日本攻读了五年的博士，最后一刻我在日本放弃了博士论文答
辩去了美国，到了美国以后我也试图在各个学校寻找一些继续通过论文转成博士的形式
，最后我在美国的叫Pacificwestern的私立大学进行 |
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c*****a
发帖数: 8464 | 48 二手交易风险自负！请自行验证是否合法和一手卡！:
Y
我想卖的物品:
住Best Western hotel折扣
单张面值:
$5. For any hotels at Best Western。
可接受价格(必须明码标价！):
你在Best Western的hotel住一次，我给你$5 cash。
可以用gc，可以用coupon，可以走任何方式check out，甚至在他家前台定都可以。
不用我帮你定，只要帮我攒stay就可以。
物品新旧要求:
邮寄方式要求:
买卖双方谁承担邮寄损失(Required if not code only):
付款方式说明:
其他补充说明:
广告的有效期:
物品来源(Required for All Cards!):
我的联系方式:
Warranty期限:
能否证明是合法的一手卡?(Required for All Cards!):
state and zip: |
|
d*******n
发帖数: 3851 | 49 【 以下文字转载自 FleaMarket 讨论区 】
发信人: dragonman (熊熊 & 投资版的牛牛), 信区: FleaMarket
标 题: 请教Best Western GC卡定宾馆，多谢！
发信站: BBS 未名空间站 (Sun Jul 11 23:59:06 2010, 美东)
俺想在priceline订个Best Western的宾馆，请教一下，怎么才能使用上Best Western
的gc呢？
另外，请教一下priceline订宾馆，大家都说bid，俺怎么没见bid啊，跟其他的网站一
样都是直接定的吧？
多谢！ |
|
d*******n
发帖数: 3851 | 50 【 以下文字转载自 FleaMarket 讨论区 】
发信人: dragonman (熊熊 & 投资版的牛牛), 信区: FleaMarket
标 题: 请教Best Western GC卡定宾馆，多谢！
发信站: BBS 未名空间站 (Sun Jul 11 23:59:06 2010, 美东)
俺想在priceline订个Best Western的宾馆，请教一下，怎么才能使用上Best Western
的gc呢？
另外，请教一下priceline订宾馆，大家都说bid，俺怎么没见bid啊，跟其他的网站一
样都是直接定的吧？
多谢！ |
|
