o**s
发帖数: 3412 | 1 I am feeding NVI dog food 13.5 oz, exact same as cat, also rotate with
wellness.
I should try Evo and Weruva again. They had diarrhea with these before, but
that could be switching from dry to can, has nothing to do with the brand.
I try to feed some raw...
I do warn you about NVI, my cats seems to really like their raw food, got
addicted to it. I am trying to wean them off it. I dun know about can, now i
just have no trust in any commercial food, other than the fact they are
convenient, or it s... 阅读全帖 |
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k*******i
发帖数: 5067 | 2 我是一个职业兽医， 在美国做了10年clinical vet。我不需要收回我说的话。
regulation act 上面写的很清楚。 你可以计算一下， 看看符合要求吗？ 我目测不符
合。我也看不惯这样养宠物。
(c) Additional requirements for dogs.
(1) Space.
(i) Each dog housed in a primary enclosure (including weaned puppies) must
be provided a minimum amount of floor space, calculated as follows: Find the
mathematical square of the sum of the length of the dog in inches (measured
from the tip of its nose to the base of its tail) plus 6 inches; then divide
the product by 144. The calculation is: (... 阅读全帖 |
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S*********n
发帖数: 4050 | 3 Beijing should be kicked out of the country. They should be weaned off all
the sweat money from people all over the country.
. |
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c*******n
发帖数: 671 | 4 you should know you need to wean off antidepressants especially for those
having short half-life. It's not relevant to whether to totally listen to
the doctor or not but to do your research and be responsible.
I learned my lessons from seeking medical help for other diseases and I don'
t trust doctors totally. Doctors are close to lairs when it comes to unknown
effects.No one's gonna take responsibility for you when the bad things
happened they'll just say to your face "I don't know".
May I ask ... 阅读全帖 |
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D*****r
发帖数: 6791 | 5 http://www.bbc.co.uk/news/10132762
'Artificial life' breakthrough announced by scientists
By Victoria Gill Science reporter, BBC News 20 May 2010
Scientists in the US have succeeded in developing the first living cell to
be controlled entirely by synthetic DNA.
The researchers constructed a bacterium's "genetic software" and
transplanted it into a host cell.
The resulting microbe then looked and behaved like the species "dictated" by
the synthetic DNA.
The advance, published in Science, ... 阅读全帖 |
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b********n
发帖数: 38600 | 6 “I’m trying to wean myself off sports, it’s too time consuming. I don’t
watch football anymore, I gave that up. I got tired of the interviews after
the games, because the winning players always give credit to God, and the
losers blame themselves. You know, just once I’d like to hear a player say,
‘Yeah, we were in the game, until Jesus made me fumble. He hates our team.
’”
–Jeff Stilson, Comedian |
|
m****r
发帖数: 1904 | 7 "Some critics and scholars have condemned Jephthah for his vow, having the
view that Jephthah followed the practice of other nations, offering up his
daughter by fire as a human burnt offering. But this is not the case. It
would be an insult to Jehovah, a disgusting thing in violation of his law,
to make a literal human sacrifice. He strictly commanded Israel: “You must
not learn to do according to the detestable things of those nations. There
should not be found in you anyone who makes his son ... 阅读全帖 |
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m****r
发帖数: 1904 | 8 申命记18：10说了耶和华痛恨把儿女投入火中。既然耶弗他是上帝悦纳的人，你认为他
会做出这样的事情吗？此时已经有摩西律法。在圣经全书里除了亚伯拉罕（没有摩西律
法）和这个值得商榷的问题以外，从来都没有拿人做全烧祭的（受异教影响除外）。利
未记第一章说明了什么可以作为全烧祭。
倒是这句话之前，先说了谁要迎接他，就把谁归耶和华。什么叫归耶和华？请参考士师
记13：5和撒母耳上1：11。实际上，耶弗他献女儿和哈拿献儿子是类似的。撒上1：4有
祭肉，1：21是献祭的时候但是撒母耳还没有断奶，也就是说如果断奶了，那么哈拿会
献人，同时按照惯例，哈拿的丈夫也会献上祭肉的。正如从撒上1：24开始描述的。
至于圣经上为什么写把他献做全烧祭，个人理解圣经公正地把当事人的想法记下来。我
们有很多时候想的是一个意思，但是说出来有可能是另外一个意思，本民族的人都能理
解，知道省略了什么，但是外族人就不一定理解，尤其是不了解这个民族的风俗习惯的
时候。从这点我倒是对圣经的记载觉得更可靠，因为它真实记载了人的不完美。
------------------------------------------------... 阅读全帖 |
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a**u
发帖数: 8107 | 9 ☆─────────────────────────────────────☆
runsun (runsun) 于 (Fri Dec 18 14:14:14 2009, 北京) 提到:
美国加州２００９.８.２９
D：科学家说数十亿年前金星曾像地球有海洋但干枯了，部分原因是金星太接近太阳，它
有水存在可能表示那里曾有物质生命，今日科学家相信，金星有微生物存在，金星是否
曾像地球能长养生命？
M：是，它过去是，它曾是美丽的星球，现在已不再是，那里完全没有生命了，没有任何
生物，很遗憾。
D：那里过去曾有生命，他们看起来像我们吗？
M：是，是，他们看起来像我们。
D：火星，地球和金星为何都有类似人类的众生居住，我们有类似的祖先吗？谢谢M。
M：我们只有一个祖先那就是上帝，有些变成堕落天使，他们看起来类似我们，是因为我
们有类似的功德，包括我们银河中的火星，金星等星球，只问我们银河中的不要问太远
的星球，否则我就不用出定了。虽然你的问题很简单，但我要查证，需要一些时间，为
了地球的生存我们也都很忙，所以我得牺牲你们的要求，一直拖到今天，我好不容易才
排进今天的行程里。
D：谢谢M
... 阅读全帖 |
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y**a
发帖数: 2546 | 10 wean的时候，一个笼子，一般一窝，6-10个
要解剖21天前的老鼠的话，一次set up 8-12 个crossing很正常
几个实验同时做的。 |
|
a****9
发帖数: 418 | 11 如果我没认错, 这是CMU Wean Hall大礼堂 |
|
p****d
发帖数: 2183 | 12 wean hall 5楼那个大的？是有点像。。 |
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h*******e
发帖数: 205 | 13 Italy's National Institute of Research on Food and Nutrition recently
published a report online in the Journal of Agricultural Food Chemistry (
read study here) documenting significant disturbances in the immune system
of young and old mice that have been fed the genetically modified corn MON
810.
The study evaluated the gut and peripheral immune response to corn in mice
in vulnerable conditions-either very young or old. Weaning and old mice were
fed a diet containing GM MON810 or its parental c |
|
D*a
发帖数: 6830 | 14 剪指头可以标记到100，有种方法可以标记上千的好像，不过很容易出错，不推荐
有人再加上切耳朵的哪里是1xx，哪里是2xx，我都是只在标签上加一个百位数，如果认
真记录，我觉得还是挺难弄错的，因为一般情况下，你不可能把101和1放到一起，
birth date差得太远了。另外老鼠尤其是公老鼠的笼子一般从weaning分笼子的时候就
固定了（母的可以重新组合，公的绝对绝对绝对不能），基本不可能一只笼子里有12，
16，24，25，另一只笼子里有112，116，124，125。
F2。 |
|
r****2
发帖数: 470 | 15 J. Agric. Food Chem. 2008, 56, 11533–11539
Intestinal and Peripheral Immune Response to
MON810 Maize Ingestion in Weaning and Old Mice |
|
D*a
发帖数: 6830 | 16 我说的就是从weaning起六个多月没接触过公老鼠的母老鼠，既然estrus cycle是正常
，proestrus，estrus，；metestrus 和 diestrus 交替规律出现，怎么叫进入
anestrus(不动情期）？
你们都是怎么测cycle length的？我都是直接拿过笼子来就测的。
我说的不会同一天下崽或者不是同一天plug是说前后差三天，差两天，差一天我都见过
，而且不少，并不是每次都差一个周期这么准。
道了
，导致怀孕不同步。 |
|
w*e
发帖数: 740 | 17 会不会是分开太小，吃不到食物，饿死的？
如果小就一个月WEAN，或者里面放MUSH FOOD + GEL WATER |
|
h*******o
发帖数: 4884 | 18 There are a few possibilities, depends on your area of interest
For me, I would check their glucose level. As rodents reliy heavily on
ketone bodies rather than glucose during suckling stage, once they are weaned they would have to
convert to a glucose-dependent phenotype.
If somehow your KO mice have defect in glucose metabolism, they will die.
And they technically die of starvation.
Just a hypothesis. If that hits your case, it will be interesting. |
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f********1
发帖数: 367 | 19 wean的时候，同一个litter的可以放一起。 |
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D*a
发帖数: 6830 | 20 set up the cage composition when weaning them. you can put males from 2
litters together at that time.
After that, NEVER regroup seperated males, they will fight until one dies. |
|
b***g
发帖数: 516 | 21 wean的时候放一块，不然尽早。也要看你的strain，有的特活跃的先来后到差几天的都
要打架。 |
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i*****g
发帖数: 11893 | 22 以前实验室一个家伙，专门做老鼠，
几乎每一个月屠掉几十只无用的老鼠，名曰：Sac
这家伙经常拿一只母老鼠上来，就在实验室bench上，杀掉，破开肚皮，拿出小球状胚
胎，做实验
老鼠屠夫
checking plug, separate, wean, recording, genotyping 这些事我都干过，但我不
是做老鼠的，没意思 |
|
S******l
发帖数: 14311 | 23 太对了，ｍｄ，每天都要mating, weaning, killing，genotyping...
垃圾ｄａｔａ。。。 |
|
d****i
发帖数: 2346 | 24 有各种品系，不同代数，不同基因的KO，不同的mate方案，wean的时间等等。
我现在用excel管理但还是感觉比较复杂比较乱，容易出错。
如果有模板的话能不能给分享一下？
谢谢！ |
|
n***w
发帖数: 2405 | 25 You can set up the breeding cage when or a couple of days after you wean
them (3 weeks).
I am not sure if you will immediately get on. |
|
l******g
发帖数: 1145 | 26 我cross了两个转基因的老鼠，发现大约1／3的老鼠从postnatal 3天到2周，出现不同
程度的体型变小，以及预期中其他的一些表型。老鼠就算只有sibling的1／2大小，但
还是生活自理，三周就可以wean了。
问题1,
现在我想quantify老鼠body size的变化，测体重是比较有效的手段吗？
我担心的是，不是所有老鼠都变小，体重平均后，也许会弱化差异。
ps，下周第一批老鼠要拉去做echo，到时麻倒了，肯定会拍比较照片。
问题2,
如果检测体重的话，需要连续测多久？可以只测到6周成年的时候吗？
concern是，现在的老鼠只是第一次cross后的double het，本来是没预期有表型的。按
计划要继续cross将一个基因完全knockout，预期得到更严重的表型。
所以说，所有的female老鼠都要用来breeding，等到怀孕后，体重肯定就不准了。就算
是公老鼠，用来breed后，都会变肥（难怪男人一结婚就发福），可能体重也不准了。
是不是测体重只能到breeding前？ |
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D*a
发帖数: 6830 | 27 小小鼠体重变化很大，尤其是weaning学吃东西的时候还可能往下降，这个就看你的表型
到底多么强了，差得多肯定就可以少用几只，不行就只能加大n了。每天测的话曲线漂
亮很多，要不你就每周测但是连测两三天作为individual的周体重，我觉得也可以。我
们测幼鼠都是每天测的，不过我们是搞growth的。我们10%的体重差，10只反正是不够.
..
isoflurane应该是常用的吧，关键是老鼠麻醉后不能趴着，正好堵住鼻孔，要侧身，把
鼻子露出来，我有惨痛教训啊 T.T
另外那个问题，初始体重是100%，不是现在体重，要不然你不都算出来降低了吗 |
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w******g
发帖数: 58 | 28 lz，你根本是在颠倒是非黑白
与其列举的论文的结论很多都不一致。您究竟读过这些论文没有？
1. BT毒素对猪猪们及大鼠的一些生理指标的影响。（Buzoianu, S.G. et al. Animal,
2012; 6(10):1609-1619）http://www.ncbi.nlm.nih.gov/pubmed/23031560
这篇文章我查不到全文，只能看摘要。
沙发说“在喂养110天后，BT玉米喂养的猪们，血清中的aspartateaminotransferase (
AST，一种转氨酶) 显著降低（P<0.05)。此外，Serum Urea在30天的时候也有显著改变
（p<0.05).”
SU的确降低了，但降低的一组是吃30天非GM食物再吃80天GM食物的那组；而其余三组包
括全部110天吃GM的组都没有降低。（On day 30, SU was lower for the non-GM/GM
treatment compared withthe non-GM, GM and GM/non-GM treatments (P < 0.05).）
这明显无法得出吃转基因导致SU改变... 阅读全帖 |
|
w******g
发帖数: 58 | 29 lz，你根本是在颠倒是非黑白
与其列举的论文的结论很多都不一致。您究竟读过这些论文没有？
1. BT毒素对猪猪们及大鼠的一些生理指标的影响。（Buzoianu, S.G. et al. Animal,
2012; 6(10):1609-1619）http://www.ncbi.nlm.nih.gov/pubmed/23031560
这篇文章我查不到全文，只能看摘要。
沙发说“在喂养110天后，BT玉米喂养的猪们，血清中的aspartateaminotransferase (
AST，一种转氨酶) 显著降低（P<0.05)。此外，Serum Urea在30天的时候也有显著改变
（p<0.05).”
SU的确降低了，但降低的一组是吃30天非GM食物再吃80天GM食物的那组；而其余三组包
括全部110天吃GM的组都没有降低。（On day 30, SU was lower for the non-GM/GM
treatment compared withthe non-GM, GM and GM/non-GM treatments (P < 0.05).）
这明显无法得出吃转基因导致SU改变... 阅读全帖 |
|
D*a
发帖数: 6830 | 30 能打死了一般是合了笼子了吧。其实我们用的b6 wean的时候放一起也没出过问题。但
是一般是推荐不要把两窝的放一起。但是再老就不行了。而且公老鼠哪怕一起在一个笼
子里的，你做实验要处理，分开了之后就不能再合了。另外发现有受伤的迹象要把出击
的那只隔离，而不是只把把受伤的那只隔离。再就是可以放mouse house 大大降低攻击
性 |
|
d****i
发帖数: 2346 | 31 如果一次买来10只以上的同龄老鼠，不可能是同窝的，来了以后很容易打架。可能最好
的办法是自己养，配对，wean，然后很小的时候就放在一起。 |
|
e********c
发帖数: 30 | 32 一般在雄鼠wean过之后，就不要在把不同litter的雄鼠放一起，打架很凶。雌鼠合笼一
般没问题。 |
|
S******9
发帖数: 2837 | 33 mating过的公鼠放一起才会打架，开始wean就放一起没关系 |
|
M*P
发帖数: 6456 | 34 just checked lior's post on Barabasi.
my conclusion is: he is just a weaning baby.
BTW, his cufflinks sucks too. |
|
T****e
发帖数: 483 | 35 有兴趣的请站内留言。
这是关于气功遥距治疗失眠的反馈
On a flight to NYC from Seattle, I was editing family pictures, and the
gentleman sitting next to me was reading a new book titled Enlightenment,
about Quantum Theory and how each action creates an immediate reaction an
inch away or a billion miles away. One of my long time salutations, found in
a Vietnamese Buddhist's writings, is "everything is everything." I know
that everything is related and that the mind works like a magnet. The
pictures I had been editing were o... 阅读全帖 |
|
T****e
发帖数: 483 | 36 看看这些实例
On a flight to NYC from Seattle, I was editing family pictures, and the
gentleman sitting next to me was reading a new book titled Enlightenment,
about Quantum Theory and how each action creates an immediate reaction an
inch away or a billion miles away. One of my long time salutations, found in
a Vietnamese Buddhist's writings, is "everything is everything." I know
that everything is related and that the mind works like a magnet. The
pictures I had been editing were of my cousin. As my s... 阅读全帖 |
|
T****e
发帖数: 483 | 37 On a flight to NYC from Seattle, I was editing family pictures, and the
gentleman sitting next to me was reading a new book titled Enlightenment,
about Quantum Theory and how each action creates an immediate reaction an
inch away or a billion miles away. One of my long time salutations, found in
a Vietnamese Buddhist's writings, is "everything is everything." I know
that everything is related and that the mind works like a magnet. The
pictures I had been editing were of my cousin. As my seat com... 阅读全帖 |
|
d*******e
发帖数: 89 | 38 2, I prefer N---wean from the ventilator
last one , I will prefer A.
Correct me if anything wrong. |
|
d*******e
发帖数: 89 | 39 In assistant ventilation, before weaning , permissive hypercapnia is
advisable. |
|
s*******i
发帖数: 48 | 40 Yeah. Agree. Also patient is awake and alert, CV signs stable, PaO2/FiO2 is
good, PEEP is low enough. I think it is time to wean. |
|
s*******r
发帖数: 174 | 41 来自主题: MedicalCareer版 - 殊途同归 这个月在icu,第一个call收的两个病人都是pneumonia/sepsis/septic shock.第一个83
岁，我收的时候他还好好的，冲我笑呢，就是sats低，lactate只有4。overnight就被
intubate拉，we did everything we need to do, 可他还是接着一天不如一天，挺了
一周，kidney开始down，platelets也开始drop，家里很快就决定withdraw care了。当
时还想着应该再给他一些时间。。。。
另外一个72，收得时候lactate7点几，一度11点几，ph7.0几，k7点几，fluids
,pressors, bicarb drip, dialysis。。。能用的全用了，当时觉得他肯定是不行的了
。。。。没想到一天一天好起来，前两天居然extubate了。。。就在我感叹年龄真是
icu病人的prognostic factor啊的时候，他还是tolerate不了拔管后的destress，面临
再度被intubate和long term weaning process， 刚上去看，已经决定comfort |
|
s***n
发帖数: 150 | 42 拍拍zhaoju妹妹，看了让人揪心。
以前从来没想过做儿科医生，自己有了小孩以后，就觉得天底下最幸福的职业就是做儿
科了，因为每天能看到那些纯真美丽的面孔。但是恐怕最残酷的也是面对小生命的夭折
而无能为力了，但可能这也是你必须经受的考验。你一定要照顾好自己，加油。
昨天也送走了一个病人。70岁的老先生服安眠药自杀，上了呼吸机，在ICU呆了两个星
期，又并发了MRSA肺炎，败血症，但情况还比较稳定。从头到尾都是我在管，走的那天
早上我们还想着可以wean了，下午我也去看了他，和他说了几句话。一个小时后护士给
他吸痰，他突然就室颤了，没有脉搏了。我lead的code，４０分钟的抢救也没有把他拉
回来。他唯一的女儿在最后一分钟赶到，当时就瘫坐在地上嚎啕大哭。Code结束后，我
很沮丧，因为实在不明白到底是啥原因，也不知道自己是不是有哪里做错了。然后我就
看到那个给他吸痰的护士在一边默默的流泪（我第一次看到ICU的护士流泪），我就问
她你还好吗？她说我不知道究竟发生了什么，自己是不是没照顾好他？我说你做的很好
（整个code过程，她都在那里给药），我们都尽力了。第二天和主治医生说了这个“意
外 |
|
l********y
发帖数: 2295 | 43 6am-7am: Arrive to the residents’ workroom to receive checkout from the
night float intern covering your service. Earlier in the year, you will
probably arrive on the earlier side of this time, but you MUST arrive prior
to 7am because this is when night float gets to leave.
Early morning before work rounds with the resident and med students: Look
through eResults and RadWeb to gather information about your patients for
the day. This includes looking at all bloodwork, radiology, and other
studies... 阅读全帖 |
|
B****r
发帖数: 1737 | 44 看了三次医生，没有任何进展。可能下周要收入院了。小地方没有儿童医院，也不知道
该不该换个别的医生。恳请大家帮着瞧瞧 （病人情况如下）。万分感谢。
5 yo female with persistent cough x 14 days and fever x 10 days. Fever runs
102-104 F, but does normalize when given motrin or tylenol. Symptom no
improvement after 4 days of Azithromycin. Began to have loose stool /watery
diarrhea for past 3-4 days. She had complained of abdominal pain (mostly
peri-umbilical) intermittently for the past 2-3 weeks. Has a history of
problems with constipation. Slightly decreased PO intake, ... 阅读全帖 |
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m******i
发帖数: 834 | 45 (SOURCE: St. Petersburg Times)
President Vladimir Putin said Wednesday that the way to wean the economy off
oil and gas was to think small.
Putin unveiled a $1 billion initiative to develop nanotechnology and turn
the Kurchatov nuclear institute into the country’s research hub for the
science.
Nanotechnology cuts across various fields of study to focus on building
devices, such as electronic circuits, from single atoms.
At least 28 billion rubles in state funds will be invested into the
Presiden |
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t*****r
发帖数: 1765 | 46 ICU的一天
My morning started with the patient with pneumonia, and my order from the physician was to wean her off the ventilator so she could breathe on her own. But this was easier said then done. When I tried to take her off the ventilator her heart rate began to increase, and her blood oxygenation level began to drop. She became agitated. At that point I knew there was no way she was getting off the ventilator that day, at least not until I could get her other symptoms under control. First, I ne |
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n********a
发帖数: 53 | 47 真心地认为版上的各位有理想, 有能力，有思想，有抱负, 以前在这里也受益不浅。今
又有一事请教：
我今年从护士加速班毕业，现在在当地排名第二的医院的Cardiac PCU工作, nursing:
Pt=1:4, Day/evening shift。The hospital is about 15 minutes drive from my
house. I am still in the orientation, but will be off it pretty soon．
Now I get another interview opportunity from the No. 1 hospital in the city.
It is an intermidiate care unit, nursing: pt=1:2, but NIGHT shift (3/week).
The pt population includes chronically ventilated patient and those who
have difficulties weaning from ven |
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a********n
发帖数: 438 | 48 我们教的是
titrate up based on onset of action
wean based on duration of action
link physiological properties of drug to patient condition |
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发帖数: 1 | 49 每个医院应该都有所谓的electrolyte protocol吧，只要医生自己order了protocol,
就可以根据protocol进行replacement. 但如何replace要根据病人的情况来制定方法。
K3.0在病人肾功能没有啥障碍的情况下给60-80都应该没啥问题，问题是根据病人的情
况如何补，比如病人本来就 GI sick, PO肯定不是好的选择，因为太难喝很有可能引起
呕吐。如果IV 给potassium普通IV给得极慢，一般给40 MEq IV 能花四小时 500 ML NS
+ KCL 130 ML/HR，但如果病人有central line access,专门有那种central access的
IVPB KCL 40MEq 100ml running at 50 ML/HR两小时就能给 40 MEq. 如果病人需要给
80只有普通IV并且没有啥恶心症状的话，给40 PO 给40 IV一般会比较好。
ARDS一般来说intensivist会尽量keep patient dry, 最有可能引起ARDS的就是
Pneumonia, 病人血压这么低是否是septic... 阅读全帖 |
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j**j
发帖数: 9 | 50 gene does be marked sometimes, of cause chemically, such as methylation, differently to maternal and parternal DNA.
what do you wean deve. is determined be the impending sperm? |
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