z**r
发帖数: 17771 | 1 that's why I said when having heavy load on the link, actually especially for
the upstream, the normal broadband server has only about 384kbps upstream,
which is so easy to get congested if you use BT or whatever service.
In this situation, Internet would not be the bottleneck, and QoS is a nice
feature. |
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z**r
发帖数: 17771 | 2 2 scenarios:
1. the RP is not on the shortest path. the upstream router will use a
different interface as the RPF check, and send a prune message to the RP
removing itself from the source tree. so on RP, the interface facing this
upstream router will be removed from the OIL. So your understanding is right.
2. the RP is on the shortest path, (s, g) will not be pruned after switching
over to SPT. of coz this depends on the spt-threshold, if the threshold is
set to infinity, the router will not eve |
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z**r
发帖数: 17771 | 3 comcast 6m/1.5m? you kidding, when I was using comcast, my downstream is 16M
, but the upstream is only 1M. For your regular 6M downstream speed, your
upstream is 384k.
到1 |
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n**********l
发帖数: 271 | 4 AS 65400 and AS65500 are peering with two links on the same router. How can
I use only one link and leave the other link as standby?
This is a customer & upstream scenario and there's nothing over the upstream
router. |
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n**********l
发帖数: 271 | 5 AS65400 customer
AS65500 upstream
BGP peering, one router at customer, one router at upstream
two circuits between routers
How to use only one link and leave the other as backup (use the backup link
only when the primary link goes down)
Why would anyone provision a circuit and only use it as backup? |
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s******e
发帖数: 493 | 6 Your problem has nothing to do with java colletcions framework. ALso I do
not think java collections framework is 高深. But that is off the topic.
Java is a strong type and object oriented language. you should be able to
find tons of articles and books talking about why an object oriented
language is better than procedural languages for enterprise applications.
Using map to save the heterogeneous class states not only defeats the
purpose of the strong type language, but also fails to mimic the dom... 阅读全帖 |
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h********n
发帖数: 4079 | 9 I mean sequencing primer.
when you design primer to sequence sth, you don't need a pair of primers. So
if I design a primer from the head of insertion and go upstream to sequence
the unknown, I only need to know 100 bp sequence. then based on this 100 bp
I can design primer, one primer on transgene and one on upstream, whose
product is cross the insertion starting point, so to distinguish homo vs
hetero.
But I don't know whether the sequencing pcr can be done on genomic DNA.
BTW, when you doubt |
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a****o
发帖数: 1786 | 10 TSS is outside of Promoter. Promoter is upstream of TSS.
For example, the sidely used T7 promoter can direct transcription of its
downstream sequence. If you try to use primer extension to determine T7
promoter, you will ger zero information. Metazoan promoters are more complex
, but promoters are still upstream of transcription start sites. |
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g***j
发帖数: 40861 | 11 Sorry, I did not explain clearly.
The template is 16 srrna. So it is likely impossible to distinguish them by
length.
The downstream primer now is hitting on thousands sequences in which about
100 sequences are not what I want. I am asking if there is a simple method
that I can blast my upstream primer in these thousand sequences. So I will
know if I can get what I want through the combination of the downstream and
upstream primers. |
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l**********1
发帖数: 5204 | 12 ZZ
老外的forum 有关的讨论帖 的最精彩的一句推论:
>So if I understood well, you suggest to lengthening the 5' end of the
construct including part of the promoter in a promoterless vector and then
transfect different cell lines and see in which one the entire transcript
will be expressed.
details pls go to
htp://molecularbiology.forums.biotechniques.com/viewtopic.php?f=2&t=31288
看看能否有助LZ的这问题的解决?
有一点务必牢记: without in vivo experimental data support for a new species 5'UTR information Blast
information is just a block ... 阅读全帖 |
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l**********1
发帖数: 5204 | 13 RE LZ
just go to
htp://www.mitbbs.com/article_t/Biology/31627067.html
11f
一句话： 要确定某个表达蛋白(A物种) 的真正的5' UTR 范围碱基序列
最好将其包括启动子部分的碱基序列转接到一个非启动子功能的载体
然后转染到异种(B物种: 可以是A物种的近缘) 的Cell line 表达那个目标蛋白
再提取那个表达后的目标蛋白
如果后者同In vivo (A物种) 的生化物理性质完全一致,
那么其包括启动子部分的碱基序列 就是真正的5' UTR 范围碱基序列
[回复]
发信人: lotkaeuler11 (Fibonacci), 信区: Biology
标 题: Re: 急求：怎样从基因组序列中找出基因的5'UTR确切序列？
发信站: BBS 未名空间站 (Thu Jan 19 05:09:42 2012, 美东)
ZZ
老外的forum 有关的讨论帖 的最精彩的一句推论:
>So if I understood well, you suggest to lengthening the 5' end of th... 阅读全帖 |
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l**********1
发帖数: 5204 | 14 LZ为了过份表达 选的promoter inserted xxxxx bases upstream of the Start codon may affect the Kozak
sequence or rbs.
then a nonsense mutation is a mutation that causes a premature stop,
resulting in a truncated or nonfunctional protein
NB: if it is my misunderstood please let me know it.
回复]
>发信人: vonda (Vonda), 信区: Biology
标 题: Re: 用qPCR检测mRNA，高1000倍，却看不到蛋白
发信站: BBS 未名空间站 (Tue Jan 31 19:01:56 2012, 美东)
天……我overexpress的是一个extensively studied的蛋白。。。没有文献报导过它不
稳定。那是阅读框错了？但是overexpress后，细胞的一些signaling pathway... 阅读全帖 |
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l**********1
发帖数: 5204 | 15 RE LZ
also 'ACGA' pls check if with or withnot
pls refer
The ribosome binding site (RBS; also known as the shine-delgarno sequence).
This is a series of nucleotides located 7-13 bases upstream of the start
codon. This sequence is recognized by the ribosome (the protein complex that
translates mRNA sequence information into protein).
from the bacteria E. coli and is:
AGGAGGA
In order to select a start codon you need to do two things;
1. Look at the various options for start codons. For each one,... 阅读全帖 |
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h***y
发帖数: 1657 | 16
前途都是虚的。在你我有生之年，还是石油。
过程工程都需要化工的。Petroleum refining可是化工人大舞台。具体的有催化剂开发
和评价，这个跟学校做的没什么大区别，就是有technician帮你动手了。
过程设计和中试操作；
过程模拟
过程控制
即使是upstream,也好多
化工的人在做，设计呀，模拟呀。你自己不就做upstream么。
一般意义上化工界不包括refining，而是包括从乙烯开始的petrochemical，
到speciality/pharmceutical。 |
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y*****a
发帖数: 580 | 17 That may not be true. For upstream, it requires more than those. Chemical
engineers could be working in the lab for say chemical flooding (ASP etc.),
but require many more knowledge in other topics, particularly geology and
multi-phase fluid flow in porous media to do a typical petroleum engineering
job. The chance for a general chemical engineer to be in upstream, (except
doing experiments), is comparably slim. I would say, thermodynamics and
simulation are useful, but not in a petroleum en |
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k*******n
发帖数: 2399 | 18 很特别的领域，从技术上来看和downdream用的差不多，不过目的却是想取代upstream,
upstream那帮人对这个领域都有很大怀疑态度。不过催化届人对此乐此不疲。。。 |
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y*****a
发帖数: 580 | 19 This is not about future of human being.
The following question is related to the questions I am asking:
(1) If PE is well paid, is it worthwhile for ppl to switch into it?
(2) Is the career unstable (as in the past) or not?
(3) What you need to do now and in the nearest future to prevent loss of
employment happening or with the minimal impact?
(4) Whether you can be enrolled in PE schools if changing study now, or if
you can launch a job in the upstream?
I am talking about one's career path and... 阅读全帖 |
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kx
发帖数: 16384 | 20 ☆─────────────────────────────────────☆
Gaojun (Gaojun) 于 (Mon Nov 14 12:04:02 2011, 美东) 提到:
Between trust and control: Developing confidence in partner cooperation in
alliancescuny.edu 中的 [PDF]…, BS Teng - Academy of management Review, 1998
- JSTOR
Strategic alliances have been recognized as arenas with potential for
opportunistic behavior
by partners. Hence, a firm needs to have an adequate level of confidence in
its partner's
cooperative behavior. In this article we examine the notion of... 阅读全帖 |
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j*****n
发帖数: 943 | 21 【 以下文字转载自 Swimming 讨论区 】
发信人: gxz (大狗熊), 信区: Swimming
标 题: zz公开水域游泳技巧
发信站: BBS 未名空间站 (Mon Jul 18 14:44:51 2011, 美东)
zz
公开水域游泳技巧
In my many years in this sport, I have never seen any reasonably
complete article in the magazines dealing with open water swimming (they
seem to rehash the same basic stuff every few years). A lot of them talk
about how to draft, or tell you to look up every few strokes to stay on
course, but very few seem to deal with the subject in much detail.
在我多年的运动生涯中，我从未在任何杂志中看... 阅读全帖 |
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j*****n
发帖数: 943 | 22 熊大转的一篇，感觉很有道理，俺再转。
【 以下文字转载自 Swimming 讨论区 】
发信人: gxz (大狗熊), 信区: Swimming
标 题: zz公开水域游泳技巧
发信站: BBS 未名空间站 (Mon Jul 18 14:44:51 2011, 美东)
zz
公开水域游泳技巧
In my many years in this sport, I have never seen any reasonably
complete article in the magazines dealing with open water swimming (they
seem to rehash the same basic stuff every few years). A lot of them talk
about how to draft, or tell you to look up every few strokes to stay on
course, but very few seem to deal with the subject in much detail.
在我... 阅读全帖 |
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|
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w*******y
发帖数: 60932 | 25 Mwave Black Color Premium HDMI V1.4 Certified Cable Male to Male Connectorr
- 6 ft # AV-HDMI-6-1.4 $1.99 f/s
Link:
http://www.mwave.com/mwave/SKUSearch.asp?px=DS&scriteria=AA7692
Mwave Premium HDMI Version 1.4 Length 6 FT
Features:
Audio Return Channel
The new specification adds an audio channel that will reduce the number of
cables required to deliver audio ?upstream? from a TV to an A/V receiver for
processing and playback. In cases where a TV features an internal content
source, such as a bui... 阅读全帖 |
|
w*******y
发帖数: 60932 | 26 Mwave Black Color Premium HDMI V1.4 Certified Cable Male to Male Connectorr
- 6 ft # AV-HDMI-6-1.4 $1.99 shipped
Link:
http://www.mwave.com/mwave/SKUSearch.asp?px=DS&scriteria=AA7692
Description:
Mwave Premium HDMI Version 1.4 Length 6 FT
Features:
Audio Return Channel
The new specification adds an audio channel that will reduce the number of
cables required to deliver audio ?upstream? from a TV to an A/V receiver for
processing and playback. In cases where a TV features an internal content
sour... 阅读全帖 |
|
c**i
发帖数: 6973 | 27 (1) Edward Wong, To Slake Thirst of North, China Looks South; Plans for
diverting 6 trillion gallons of water a yearn. New York Times, June 2, 2011
(title in print).
http://www.nytimes.com/2011/06/02/world/asia
/02water.html?scp=1&sq=china%20yellow&st=cse
"Some Chinese scientists say the diversion could destroy the ecology of the
southern rivers, making them as useless as the Yellow River. The government
has neglected to do proper impact studies, they say. There are precedents in
the United Stat... 阅读全帖 |
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t*******h
发帖数: 2882 | 28 刚看到的，三大铁矿石成本价每吨20-25美元，卖给中国150美元
http://www.focac.org/eng/zxxx/t706530.htm
"The cost of iron ore is only $20 to $25 per ton, but the big three miners
are selling it at $150 per ton. Chinese companies need to invest in the
upstream sector to have better cost prices," |
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C********n
发帖数: 6682 | 29 S.D. federal jury: Oregon man threatened president
By The Associated Press
09.09.02
Printer-friendly page
A federal jury in Sioux Falls, S.D., took just over an hour to return a
guilty verdict last week in the case of an Oregon man accused of making
threats against President Bush.
Meanwhile, a Pennsylvania man says he'll fight disorderly conduct charges
stemming from his arrest during Bush's visit to that state last week.
In the South Dakota case, Richard Humphreys, of Portland, Ore., was arrest... 阅读全帖 |
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c**i
发帖数: 6973 | 30 VOA Chinese, Mar 22, 2011.
http://www.voanews.com/chinese/news
/20110322-India-China-Summit--118422814.html
(印中可能发生潜在水资源争执)
Note:
(a) 世界水日 World Water Day
(b) For 雅鲁藏布江, see Brahmaputra River
http://en.wikipedia.org/wiki/Brahmaputra_River
(Its upstream in Tibet is called Yarlung Tsangpo River; into Arunachal
Pradesh 阿鲁纳恰尔 邦; merges with the Ganges; While most rivers on the
Indian subcontinent have female names, this river has a rare male name, as
it means "son of Brahma" in Sanskrit (putra means... 阅读全帖 |
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R*I
发帖数: 1840 | 31 小白兔这个冤啊，人家本来是人畜无害
http://www.ft.com/cms/s/0/4f19a01e-d2f1-11e0-9aae-
00144feab49a.html#axzz1Wc29O400
Water is the new weapon in Beijing’s armoury
By Brahma Chellaney
The writer is a professor at the independent Centre for Policy Research
in
New Delhi and author of Water: Asia’s New Battleground
China has aroused international alarm by using its virtual monopoly of
rare
earths as a trade instrument and by stalling multilateral efforts to
resolve
disputes in the South China Sea. Among its neighbou... 阅读全帖 |
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x****u
发帖数: 12955 | 32
By controlling the resource upstream, controlling the market channel
downstream, and controlling the technology through IP protection. China's
manufacturing sector was completely squeezed out of any profit potential.
Since it's not profitable, it can't grow normally. That's why almost
everybody in China was playing in the real estate market. Because that's
the only way to make money. Now the entire manufacturing sector is hijacked
by this financial bubble.
Burst this bubble, then the enti... 阅读全帖 |
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x****u
发帖数: 12955 | 33
Learn to read.
"２ 是因为上游越来越多的水土流失而带来的泥沙淤集。你可以定期放水清淤，但问题
是你清淤的时候，短时间内下游江水的泥沙大增，很多将沉积的下游，就象黄河一样，
速度可能没有黄河快，但加以时日，长江很可能变成两一个河上河。"
I doubt this is talking about upstream. |
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l******t
发帖数: 55733 | 34 啥意思啊也没电梯。矮牛牛是屁歪最主要的信息链upstream连接结界。
大家都知道为啥屁歪不来了把 |
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T**E
发帖数: 1892 | 35 美国《华盛顿邮报》7月10日爆料称，除了“棱镜”计划外，美国情报机构还有一个名
为“上游”（Upstream）的监控项目，通过美国周边的海底光缆搜集情报。
这篇报道公布了一张美国国家安全局的机密幻灯片，其中对“棱镜”计划、以及与之平
行展开的“上游”计划有所介绍。
我老一直不理解防火长城和方校长，有时候还恶语中伤，现在终于明白了。
我老愿负荆请罪。
http://www.washingtonpost.com/wp-srv/special/politics/prism-col
在这一张最新公布的演示图中，上半部分蓝色框内是“上游”项目，是从“海底光缆等
基础设施收集数据”。图片下半部分绿色框内解释“棱镜”计划是通过谷歌、微软、脸
谱、雅虎、Skype、PalTalk、Youtube、苹果、和美国在线等9家互联网企业挖掘数据。
“棱镜”计划相当于“下游”项目，因为收集的是经过科技公司加工的数据。
幻灯片还用黄色圆圈提醒国家安全局人员“应利用两个项目”。
这张幻灯片中使用的背景是一张粗略的北美地图，其中显示了从美国东西海岸延伸至世
界各地的深海光缆路线。海底光缆对... 阅读全帖 |
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h**z
发帖数: 9751 | 36 【 以下文字转载自 Texas 讨论区 】
发信人: hznz (hznz), 信区: Texas
标 题: Re: 看不下去了,匿名写一下油工的家庭收入 (转载)
发信站: BBS 未名空间站 (Fri Jul 24 19:01:00 2015, 美东)
ls在胡呲牛逼，今年不知道，但前2年Exxon upstream research的PhD起薪不超过11万
，统一价，不是因为老中没身份就这价，红博Phd一样。谁胡说谁是王八。
嘴炮索南索女工资吹得山响，仿佛别人都不知道有个叫glassdoor的网站一样。吹个个
工资都狂高不知道是什么心态，一个个这么牛逼，其实你们想想周围的人的家里摆得都
是什么破烂货陈设，开得什么破车，中午带了一盒大蒜韭菜大葱味的前天剩饭，不就是
露馅了？天天就是保值保值保值，省油省油省油。为啥呢，在油公司油服公司拿的那
PHD工资，在休斯敦也不经花，还得省。是不是实话？ |
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O*******d
发帖数: 20343 | 37 http://www.usatoday.com/story/news/world/2015/10/09/why-china-d
文章要点：
For starters, China doesn’t need to belong to the TPP to enjoy some of the
perks that come with being a member.
Beijing already has free trade agreements with more than half of the TPP
countries, including Australia, New Zealand, Chile, Peru, Singapore, Brunei
and Vietnam, and it can exploit those arrangements to minimize or avoid
import duties that would normally apply to made-in-China products.
“To satisfy certain country--o... 阅读全帖 |
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w********t
发帖数: 12853 | 38 How Turkey Exports ISIS Oil To The World: The Scientific Evidence
Tyler Durden's pictur
eSubmitted by Tyler Durden on 11/27/2015 15:00 -0500
B
... 阅读全帖 |
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r***e
发帖数: 2000 | 39 XE now confirm that upstream providers are affirming this rate (7.48 RMB to
USD currently) |
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T********N
发帖数: 2680 | 40 upstream providers
是指交易中心还是我狗？
to |
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O*******d
发帖数: 20343 | 41 张纯如的silkworm, 指中国的“蚕"导弹。 中国自己从来没有给自己的导弹命名“蚕”
https://en.wikipedia.org/wiki/Silkworm_(missile)
The Shang You or SY-series (Chinese: 上游; pinyin: shàngyóu; literally: "
Upstream"), and the Hai Ying or HY-series (Chinese: 海鹰; pinyin: hǎiyīng;
literally: "Sea Eagle") were early Chinese anti-ship missiles. They were
derived from the Soviet P-15 Termit missile.
The HY-1 and HY-2 received the NATO reporting name Silkworm. However, to
confuse matters, Western media also referred to the SY-series, and its... 阅读全帖 |
|
b********n
发帖数: 38600 | 42 iPhone Disappointment Hammers Suppliers
https://www.bloomberg.com/news/articles/2017-09-25/iphone-disappointment-
hammers-suppliers-fuels-taiwan-outflows
As Digitimes reports, Tim Cook's company has instructed component suppliers
to "withhold part of the component shipments prepared for the production of
iPhone X devices" according to unidentified Taiwan-based upstream suppliers. |
|
发帖数: 1 | 43 美军是二傻子吗？找冰山干啥？
The Japanese government took full responsibility for sinking Panay but
continued to maintain that the attack had been unintentional. Chief of Staff
of Japanese naval forces in northern China, Vice Admiral Rokuzo Sugiyama,
was assigned to make an apology.[7] The formal apology reached Washington, D
.C. on Christmas Eve.
Although Japanese officials maintained that their pilots never saw any
American flags on Panay, a US Navy court of inquiry determined that several
US flags were cle... 阅读全帖 |
|
发帖数: 1 | 44 The last gorge, Xiling, is located upstream of Yichang and extends for a
distance of 21 miles; in places limestone cliffs rise directly out of the
water, although with the rise of the reservoir to much lower heights than
before. The gorges are rocky, and the walls are speckled with cracks, niches
, and indentations. Even before the river was inundated, its depth in the
gorges was considerable, increasing to between 500 and 600 feet (150 and 180
metres) and giving the Yangtze the greatest depths ... 阅读全帖 |
|
发帖数: 1 | 45 The section of river from the mouth to 250 miles (400 km) upstream is
subject to the influence of tides. The maximum range of the tides near the
mouth is 13 to 15 feet (4 to 5 metres). The Yangtze delta is rich in mud and
silt and is dominated by fluvial and tidal processes.
The present-day bed of the Yangtze in this area is somewhat above the
elevation of the plain. Thus, to protect the surrounding region from
floodwaters, the banks of the main and other rivers are built up; the total
length of... 阅读全帖 |
|
s***c
发帖数: 1926 | 46 CNN已经在回忆武汉往日的荣光了。
From one-time Chinese capital to coronavirus epicenter, Wuhan has a long
history that the West had forgotten
By Paul French
Updated 5:51 AM EST, Sun February 23, 2020
(CNN) Before the novel coronavirus outbreak hit Wuhan in December, the exact
whereabouts -- and even existence -- of the central Chinese city had
slipped from the general public's awareness in the West.
But it wasn't always that way.
Two generations ago, this city of 11 million people, on the junction of the
Yangt... 阅读全帖 |
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n******e
发帖数: 708 | 47 I hear China spent more money on importing chips than oil
China's tech level is like sea level, any countries below that can't live
well
(i.e, get higher than market return).
If China could rely on its own chips, even a fraction of its needs, the
influence will be foundmental. Gradully the upstream and downstream industry
will develop around that, which will be a nightmare for US tech companies,
which is centered around chipmaking.
maybe not be 20% for now, but in the long run, it will drag down... 阅读全帖 |
|
M*V
发帖数: 3205 | 48 美国《华盛顿邮报》7月10日爆料称，除了“棱镜”计划外，美国情报机构还有一个名
为“上游”（Upstream）的监控项目，通过美国周边的海底光缆搜集情报。
这篇报道公布了一张美国国家安全局的机密幻灯片，其中对“棱镜”计划、以及与之平
行展开的“上游”计划有所介绍。
我老一直不理解防火长城和方校长，有时候还恶语中伤，现在终于明白了。
我老愿负荆请罪。
http://www.washingtonpost.com/wp-srv/special/politics/prism-col
在这一张最新公布的演示图中，上半部分蓝色框内是“上游”项目，是从“海底光缆等
基础设施收集数据”。图片下半部分绿色框内解释“棱镜”计划是通过谷歌、微软、脸
谱、雅虎、Skype、PalTalk、Youtube、苹果、和美国在线等9家互联网企业挖掘数据。
“棱镜”计划相当于“下游”项目，因为收集的是经过科技公司加工的数据。
幻灯片还用黄色圆圈提醒国家安全局人员“应利用两个项目”。
这张幻灯片中使用的背景是一张粗略的北美地图，其中显示了从美国东西海岸延伸至世
界各地的深海光缆路线。海底光缆对世界范围内的数据传播方面极为... 阅读全帖 |
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B**W
发帖数: 2273 | 49 Ports along the U.S. East and Gulf Coasts are racing to expand so that they
can take on an anticipated growth in shipping once an expanded Panama Canal
opens.
New locks are expected to be completed at the Panama Canal in 2014. They’ll
enable much larger ships to pass through the canal, providing these ships
to take direct routes between Asia and the U.S. East Coast.
“It will dramatically change the dynamics of shipping to the East Coast, or
at least that’s the expectation of some in the industry... 阅读全帖 |
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