T**********r
发帖数: 287 | 2
inducible
多谢提醒，不过你提到这个是openbiosystem的另外一个系统TRIPZ，的确是用
tetracyline
induce的。
Open biosystem有两种shRNAmir系统实现knock down:
a.Stable knockdown: GIPZ；shRNAmir is constitutively expressed as a
polycistronicvector that codes for tGFP, puromycin resistance
marker (Puror) and the shRNAmir.
b.Regulated knockdown: pTRIPZ,也是你提到的。
见链接
http://www.openbiosystems.com/collateral/rnai/pi/Lentiviral%20shRNAmir%2
0for%20Stable%20and%20Regulatable%20RNAi.pdf
再次真诚感谢，有了质疑，才能避免将来实验走弯路。 |
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