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全部话题 - 话题: tamra
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s******t
发帖数: 2374
1
nod
她最真实, good or bad,她忠于自己的感受和看法
恩,12月吧,就分居离婚了
还是simon给file的,这个男人老是怪人家vicky教坏了tamra,这是哪儿跟哪儿啊??
?tamra是个思想独立的女人,她在他的control下生活那么多年,她自己没想法么?
vicky是个女强人,自然就更会同情tamra的悲剧了。。。
W*****B
发帖数: 4796
2
来自主题: Military版 - 有谁听说过闭锁综合症?
这个病人居然康复了
'I Was a Prisoner in My Own Body': One Man’s Incredible Recovery
From Locked-In Syndrome
Jeff Truesdell
Mike Dils’ slurred words and weakness that led his wife, Cheryl, to take
him to the urgent care clinic on Feb. 4, 2012, gave them no hint of what was
to come.
In the waiting room he passed out. After EMTs revived him, he flatlined at
the hospital. Within hours the vibrant realtor, then 64, was unable to move,
breathe or speak on his own. He became the paralyzed victim of a stroke. In... 阅读全帖
n********e
发帖数: 41
3
来自主题: JobHunting版 - Leetcode的系统真是弱爆了
楼主 看到 你 isDiffByOne 函数就知道你必然要超时
Leetcode免费给你用 就别挑剔了。凡事要先从自身找问题
你那个测试不是大数据
真正的大数据在这里:
start = "nanny";
end = "aloud";
String[] d = {"ricky","grind","cubic","panic","lover","farce","gofer
","sales","flint","omens","lipid","briny","cloth","anted","slime","oaten","
harsh","touts","stoop","cabal","lazed","elton","skunk","nicer","pesky","
kusch","bused","kinda","tunis","enjoy","aches","prowl","babar","rooms","
burst","slush","pines","urine","pinky","bayed","mania","light","flare","
wares","wom... 阅读全帖
s******t
发帖数: 2374
4
来自主题: CouchPotato版 - the real housewives, orange county
今天晚上新的一集
竟然被vicki的letter打动了。。。 她虽然有时候太女强人了,但她是个挺真实的人。
tamra挺可怜的。。。我挺喜欢她,不喜欢她男人。
y*****a
发帖数: 6993
5
来自主题: CouchPotato版 - the real housewives, orange county
vicki看不起人还需要理由吗,呵呵,这些家庭妇女最不需要找理由的就是看不起谁谁
谁,跟谁闹决裂,跟谁好好打一场架吧,tamra和ramona算是最好的例子。

的,
s******t
发帖数: 2374
6
我以前很喜欢她的
现在,还谈不上马上转变看法。。。
也许jill确实太demanding,她要她找个hobby也没什么不对
我倒是对昨晚的OC那一集很有兴趣。
我很可怜tamra
她男人确实是个asshole,很讨厌他
f*********g
发帖数: 3319
7
我也看了,觉得tamra是那群女人里最真实的一个,本来觉得她和老公还不错,谁想到
变成这样。最后说他们离婚了
C******d
发帖数: 362
8
reporter dye: 6-FAM
Quencher: TAMRA
I collect data at the end of annealing.
我设定的检测信号:ROX和FAM, ROX is reference
这些天,我就只准备了两个样品,一个NTC,一个添加了cDNA的,看是否能得到扩增曲
线,但结果都总是没有,我还改变了probe的浓度
仪器设置上,你觉得还有哪些应该注意的?谢谢
s****2
发帖数: 4569
9
来自主题: Chemistry版 - 求助--关于有机物荧光特性
how much/how many nm of 红移或蓝移 you are talking about?
eg. say from -NMe2 (of TAMRA) group to -NEt2, you many 红移 6-10nm!
c******d
发帖数: 564
10
来自主题: Chemistry版 - 荧光光谱问题请教
我是外行,有个关于荧光光谱的问题请教。 比如下面这大约十种荧光集团,单靠光谱
分析能分出来吗?
或者转化成另外一个问题,MAx EM 只差 5, 6 nm,这两个基团能靠光谱分析分开吗?
溶液环境一致但是较复杂,是PCR反应产物,里面有0.5mM MgCl2,0.05mM dNTP,DNA,
痕量protein等。
多谢指点
Dye Max. EX (nm) Max.EM (nm)
6-FAM 494 515
JOE™ 520 548 BHQ-1,
TET 521 536
Cal Fluor® Gold 5401 522 541
HEX2 535 555
Cal Fluor Orange 5602 540 561
TAMRA™ 555 576
Cy3® 550 570
Quasar® 5703 548 566
Cal Fluor Red 5904 565 588
ROX™ 573 602
Texas Red® 583 603
Cy5® 651 674
Quasar 6705 647 667
Cy5.5... 阅读全帖
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