A******y
发帖数: 2041 | 1 But there are a lot papers literally are looking at SIRT5 and SIRT6 as
deacetylases (they are
not!) Just becasue you artificially increase the substrate concentration in
a test tube and get some activity, does not mean they are deacetylase. Btw
, there are Cell papers look at SIRT5 as deacetylase in-vivo, and in my
opinion, they are looking at other SIRT or HDAC that binds SIRT5. SIRT and
HDACs are all in a huge complexes.
Btw, the same thing for HDAC class IIa, HDAC8, HDAC6 domain 1, HDAC10,... 阅读全帖 |
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A******y
发帖数: 2041 | 2 Google SIRT5 and succinate etc. SIRT6 although has been reported to be a
deacetylase, but I think it is BS (don't worry no paper will be retracted,
just like all the class IIa HDAC paper). The activity is too low or none.
I think class IIa, IV, HDAC6 domain 1, and SIRT4, 6, and 7 substrates are
either not acetyl group or require special activation and highly context
dependent.
N.M. here is the link: http://www.ncbi.nlm.nih.gov/pubmed/22076378
P.S. I'm one of the reviewers of the SIRT5 paper. |
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P*******e
发帖数: 27 | 3
SIRT5 is kind of special since it has tyr and Arg in the substrate site.
These residues can interact with the carboxylate of succinate. I am not sure
you can make such a generalization.
If SIRT6 works through regulation of transcription, probably it doesn't need
to be very active in terms of deacetylation. As long as H3K56Ac at the
promoter of target genes can be selectively deacetylated, the effect can be
amplified by the transcription process assuming the acetylation is slow. |
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s*******s
发帖数: 132 | 4 你要说的是乙酰，不是甲基
你给的文章恰恰说明了Sirt5不是deacetylase，是deacylase |
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A******y
发帖数: 2041 | 5 Yes, let's hunt down all papers telling you that Sirt5 is a deacetylase and
tell the PI to retract. Same things for HDAC classIIa etc. |
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s*******s
发帖数: 132 | 6 那说说你对于Sirt5和6两篇文章的看法？呵呵，我想看看大家的想法
越critical的越好 |
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A******y
发帖数: 2041 | 7 They are good. At least the SIRT5 one. It can be reproduced by several
other labs. SIRT6 is new, but we will see. |
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S******i
发帖数: 71 | 8 1
Lysine Glutarylation Is a Protein Posttranslational Modification Regulated
by SIRT5.
Cell Metab. 2014 Apr 1;19(4):605-17. doi: 10.1016/j.cmet.2014.03.014.
2.
Lysine 2-hydroxyisobutyrylation is a widely distributed active histone mark.
Nat Chem Biol. 2014 May;10(5):365-70. doi: 10.1038/nchembio.1497.
3
Identification of 67 histone marks and histone lysine crotonylation as a new
type of histone modification.
Cell. 2011 Sep 16;146(6):1016-28. doi: 10.1016/j.cell.2011.08.008.
4
The first identific... 阅读全帖 |
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