k****4
发帖数: 43 | 1 这个跟mean不mean没什么关系。我06年的时候找人要一个质粒，那人说有空帮我
找找。结果4年多了还没见他有空。估计压根就忘了。
鼠造血干细胞。查到文献有些实验
记错了，ATCC没的买，可以再找他
Unfortunately we don't have enough
give some away.In this case, I'll let
，类似CHO，转进去了mouse SCF
给老板，是不是这个学生怕麻烦，自作 |
|
O******e
发帖数: 4845 | 2 很正常的事情。是你在求他，不是他求你。Just be patient or ask for it from
somewhere else.
鼠造血干细胞。查到文献有些实验
记错了，ATCC没的买，可以再找他
Unfortunately we don't have enough
give some away.In this case, I'll let
，类似CHO，转进去了mouse SCF
给老板，是不是这个学生怕麻烦，自作 |
|
f****b
发帖数: 2410 | 3 客观环境也推波住蓝，获诺奖的钱社么就是问一个白人要的荧光物质，那个白人现在在
开出租车生
活，诺奖连提都没有提，现在的人文环境太差了，生物就别提了，
鼠造血
干细胞。查到文献有些实验
记错了，
ATCC没的买，可以再找他
Unfortunately
we don't have enough
then give some away.In this case, I'll let
，类似CHO，
转进去了mouse SCF
给老板，是不
是这个学生怕麻烦，自作 |
|
s****9
发帖数: 932 | 4 It is norm.
I would like to share an experience also. We want a cell line from a group.
They said yes but need to charge us $250 for the cell culture associated
expense plus shipment. We want it badly at that time and paid them $250 in
the end.
鼠造血干细胞。查到文献有些实验
记错了，ATCC没的买，可以再找他
Unfortunately we don't have enough
give some away.In this case, I'll let
，类似CHO，转进去了mouse SCF
给老板，是不是这个学生怕麻烦，自作 |
|
w*****r
发帖数: 2061 | 5 还不错了，有的直接不回复
鼠造血干细胞。查到文献有些实验
记错了，ATCC没的买，可以再找他
Unfortunately we don't have enough
give some away.In this case, I'll let
，类似CHO，转进去了mouse SCF
给老板，是不是这个学生怕麻烦，自作 |
|
b**j
发帖数: 415 | 6 mean is usual, nice is unusual regarding this.
鼠造血干细胞。查到文献有些实验
记错了，ATCC没的买，可以再找他
Unfortunately we don't have enough
give some away.In this case, I'll let
，类似CHO，转进去了mouse SCF
给老板，是不是这个学生怕麻烦，自作 |
|
a*********n
发帖数: 2526 | 7 有啊,SCF 的低物需要被在保守为电磷酸化,才能被E3 识别和泛素化 |
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l**********1
发帖数: 5204 | 8 Re LZ
pls refer one PhD dissertation
by Gernot Guderian (2010) of Basel University
title:
"Control of Centriole Numbers by Plk4 Autophosphorylation and βTrCP-
mediated Degradation"
link:
//edoc.unibas.ch/1233/
or
PDF link:
//edoc.unibas.ch/1233/1/Thesis_Gernot_Guderian.pdf
its pp25
2.5.2
The SCF^βTrCP Complex
To achieve high substrate specificity, cells express many different E2
enzymes (about 30)
and even more E3 ligases (more than 300).
The latter are categorized into four major
classes accor... 阅读全帖 |
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S*********s
发帖数: 304 | 9 请教做cell cycle的大拿~
有没有在mitotic phase or cytokinesis过程中stablize的蛋白~最好是通过post-
translational来调节的
如APC control G1 phase,SCF control S/G2/M phase的蛋白~
Thanks |
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m********2
发帖数: 317 | 10 Nat Biotechnol. 2014 Sep;32(9):941-6. doi: 10.1038/nbt.2951. Epub 2014 Jun
22.
Generation of mouse models of myeloid malignancy with combinatorial genetic
lesions using CRISPR-Cas9 genome editing.
给通讯作者写信问了，然后第一作者的回复是这样的:
for the original virus we have been using standard calcium phosphate
transfection wit plasmid ratios of 6:10:1.5 (all ug per 10 cm dish) for
vector:psPAX2:pMD2.G.
The virus was collected at around 24-28h and 36-40h post transfection and
concentrated 50-100x with ultrazentrifuga... 阅读全帖 |
|
|
n******e
发帖数: 4 | 12 BUCT has recently initiated a high level research & development programme in
Supercritical Fluid (SCF) and Dense Gas Technologies (DGT), based in the
College of Chemical Engineering. As part of this initiative, BUCT has
created 3 new Faculty positions plus a position for an overseas researcher.
The major emphasis of the research programme will be on the re-formulation
of pharmaceuticals to improve bioavailability and facilitate alternative
modes of drug delivery. A significant focus of the re |
|
L*****s
发帖数: 24744 | 13 Call for Papers
Conference Introduction
Welcome to International Conference on Applied Chemistry and Chemical
Engineering (ACCE2016).
2016 International Conference on Applied Chemistry and Chemical Engineering
will be held in Suzhou, China, on June 4 – 5. This conference covers all
branches of applied chemistry and chemistry engineering. It aims to provide
a platform for researchers, engineers, academicians as well as industrial
professionals from all over the world to present their research res... 阅读全帖 |
|
g*********n
发帖数: 808 | 14 我的理解是这样的：
基本上我们不用太管scf 的convergence.因为程序会自动控制。如果有中途死机，是因
为计算所耗内存太大，而非是收敛问题所致。
具体到一个物理性质例如说能量，我们可以调节一些参数直到能量不再依赖我们所用的
参数，我们可以说，我们算得的结果是收敛的？
一般而言，是否有一些例行的steps 我们可以follow up？ 例如说我们想要基态能，
先设计结构，接着优化结构，再算能量？ |
|
j*********m
发帖数: 33 | 15
，
we usually don't change scf convergence criteria unless we know what we
are doing.
For siesta and bulk copper, two parameters need your attention, mesh
cutoff and k-points. let's assume you want to calculate lattice constant
of copper bulk, you need to increase mesh cutoff and k-points until
lattice constant doesn't change.
I am not very familiar with real space codes like siesta, there may be
some subtle issues with numerical basis set which I am not aware of. Last
but not least, you may nee... 阅读全帖 |
|
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|
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c******r
发帖数: 76 | 19 对，我有一个计算就是出现了你说的spin-contamination的error，
SCF Done: E(UB3LYP) = -1836.07285467 A.U. after 129 cycles
Convg = 0.1181D-05 -V/T = 2.0399
= 0.0000 = 0.0000 = 0.5000 = 0.9628 S= 0.6013
= 0.000000000000E+00
Annihilation of the first spin contaminant:
S**2 before annihilation 0.9628, after 0.7629
Convergence failure -- run terminated.
这就表示我的multiplicity设置错误了？
谢谢 |
|
L*****s
发帖数: 24744 | 20 Call for Papers
Conference Introduction
Welcome to International Conference on Applied Chemistry and Chemical
Engineering (ACCE2016).
2016 International Conference on Applied Chemistry and Chemical Engineering
will be held in Suzhou, China, on June 4 – 5. This conference covers all
branches of applied chemistry and chemistry engineering. It aims to provide
a platform for researchers, engineers, academicians as well as industrial
professionals from all over the world to present their research res... 阅读全帖 |
|
u*****0
发帖数: 156 | 21 Need my help to set you up? Shoot me some
pictures of yours and your stats. I will hook you guys up. |
|
n******e
发帖数: 4 | 22 【 以下文字转载自 ChemEng 讨论区 】
发信人: NanoFace (nanoface), 信区: ChemEng
标 题: 北京化工大学事业编制正式教师招聘（化工学院）（诚聘海内外英才）
关键字: 北京化工大学,纳米,医药,招聘
发信站: BBS 未名空间站 (Mon Jul 19 03:14:49 2010, 美东)
BUCT has recently initiated a high level research & development programme in
Supercritical Fluid (SCF) and Dense Gas Technologies (DGT), based in the
College of Chemical Engineering. As part of this initiative, BUCT has
created 3 new Faculty positions plus a position for an overseas researcher.
The major emphasis of the research |
|
n******e
发帖数: 4 | 23 【 以下文字转载自 ChemEng 讨论区 】
发信人: NanoFace (nanoface), 信区: ChemEng
标 题: 北京化工大学事业编制正式教师招聘（化工学院）（诚聘海内外英才）
关键字: 北京化工大学,纳米,医药,招聘
发信站: BBS 未名空间站 (Mon Jul 19 03:14:49 2010, 美东)
BUCT has recently initiated a high level research & development programme in
Supercritical Fluid (SCF) and Dense Gas Technologies (DGT), based in the
College of Chemical Engineering. As part of this initiative, BUCT has
created 3 new Faculty positions plus a position for an overseas researcher.
The major emphasis of the research |
|
L*****s
发帖数: 24744 | 24 Call for Papers
Conference Introduction
Welcome to International Conference on Applied Chemistry and Chemical
Engineering (ACCE2016).
2016 International Conference on Applied Chemistry and Chemical Engineering
will be held in Suzhou, China, on June 4 – 5. This conference covers all
branches of applied chemistry and chemistry engineering. It aims to provide
a platform for researchers, engineers, academicians as well as industrial
professionals from all over the world to present their research res... 阅读全帖 |
|
