w*********g
发帖数: 30882 | 1 Un-White America: Will Blacks and Hispanics lead the USA to ruin
http://www.theburningplatform.com/?p=40398
I think ol’ Fred may be on to something here. Does that make me a racist?
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I begin to weary of the endless denunciations of “white privilege;” the
calls to end the dominance of malignant white men; the law suits to abolish
all tests on which white men excel, meaning almost any test of mental
ability; to make universities and police forces and advanced-placement
clas... 阅读全帖 |
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p***n
发帖数: 17190 | 2 這個我有研究過一點點
關鍵在水
水是 HOH
然後是碳
COH
COH 接在一起就是糖
purine pyrimidine
是碳氮合
碳好說話
要抓氮就得雙鍵不然氮不開心會兩個氮跑掉自己開小灶
胺基酸
則是氮碳合加上水的-OH
所以一切的關鍵
是水
上善若水
男人是水做的
女人的脂肪含量比較高 |
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x*****o
发帖数: 441 | 3 求文献, 是中国杂志, 没有知网账号.
Synthesis and crystal structure of 2-benzylsulefnyl-3-cyano-5,7-dimethyl-
pyrazolo[1,5-alpha]pyrimidine. Chinese J Struc Chem. 2004;23:267-9.
多谢! |
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w******e
发帖数: 1187 | 4 明白了。好奇问一下，如果xcription时就加radio的话，你们接下来的
phenol/PAGE/elute都在radio room里做吗？感觉确实有点吓人呵呵。
不过这样每个xcript有多个radio label，intensity一定够了：）
遗憾我要用2'-F pyrimidine，只能用epicenture的kit。还是要多谢推荐！ |
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l**********1
发帖数: 5204 | 5 Sure
Nature (2009)
Synthesis of activated pyrimidine ribonucleotides in prebiotically plausible
conditions.
//www.ncbi.nlm.nih.gov/pubmed/19444213
and
CSHL review (2010)
Closing the Circle: Replicating RNA with RNA
//cshperspectives.cshlp.org/content/2/10/a002204.short |
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l**********1
发帖数: 5204 | 6 Sure
Nature (2009)
Synthesis of activated pyrimidine ribonucleotides in prebiotically plausible
conditions.
//www.ncbi.nlm.nih.gov/pubmed/19444213
and
CSHL review (2010)
Closing the Circle: Replicating RNA with RNA
//cshperspectives.cshlp.org/content/2/10/a002204.short |
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p*****e
发帖数: 332 | 7 我想主要原因是因为有些ORF是反向的原因，所以那种定义就不太准了，后来慢慢
的就不太用watson和Crick了，这篇review写的相对比较清楚了，总的感觉是，w
atson是通常写法中的上链（5‘-3’），Crick是下链（3'-5'），而通常上链是
用来做转录模板的,便被定义成了了antisense了。共同进步，hand!
http://www.biology-direct.com/content/6/1/7
Definition Watson Crick
Original cytosine-poor cytosine-rich
Compositional pyrimidine-rich purine-rich
Transcriptional antisense sense
Replicational lagging leading
Arbitrary this that
Database top/plus ... 阅读全帖 |
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l**********t
发帖数: 5754 | 8
Nucleotide codes
A Adenine
G Guanine
C Cytosine
T Thymine
U Uracil
R Purine (A or G)
Y Pyrimidine (C or T)
N Any nucleotide
W Weak (A or T)
S Strong (G or C)
M Amino (A or C)
K Keto (G or T)
B Not A (G or C or T)
H Not G (A or C or T)
D Not C (A or G or T)
V Not T (A or G or C) |
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y****z
发帖数: 92 | 9 Could anybody please help me download this paper? Thank you!
"Reaction of acetyl hypofluorite with pyrimidines. Part 3. Synthesis,
stereochemistry, and properties of 5-fluoro-5,6-dihydropyrimidine
nucleosides"
J. Chem. Soc., Perkin Trans. 1, 1988, 2547-2554 |
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