发帖数: 1 | 1 9月28日Joe Miano
Most of you have already moved on from this discussion but I wanted to chime
in one last time with our negative data in the mouse. Whether we used
original plasmid or a custom NgAgo that was codon-optimized for mouse with
untranslated sequences, a Kozak Methionine, and polyA tail the results were
uniformly negative. We used a 5' phosphorylated ssDNA (both IDT bought or
in vitro phosphorylated) along with the mRNA to codon-optimized NgAgo and an
HDR template we used successfully ... 阅读全帖 |
|
h*h
发帖数: 18873 | 2 Elephant Seal Pups, South Georgia Island
Fattened and then abandoned by mothers who leave to mate anew, weaned
elephant seal pups stick close together until ready for a first season at
sea. |
|
k********k
发帖数: 5617 | 3 加拿大西部育空地區白馬鎮家庭在湖邊撿了一隻“狗崽子”，要為他找了一個狗奶媽。
把他送到了育空地區野生動物保育局。由保育員為他每三個小時喂一次奶瓶。但是發現
他不像小狗。於是猜想他是一隻水獺，水貂，狼獾。結果都不是。專家說，從他的尾巴
白色末端看，這是一隻幼狐。
Whitehorse family's rescued puppy turns out to be a fox
http://www.youtube.com/watch?v=EWtbuBPTkWA
Published on Apr 25, 2014
A Whitehorse family got a little wild surprise this week when it turned out
the abandoned puppy they rescued wasn't a puppy at all.
The baby animal was in poor shape when it was discovered abandoned near
Marsh Lake over the Easter weekend.
"I j... 阅读全帖 |
|
w*******y
发帖数: 60932 | 4 CDNN Sports, Inc.
Gun, Mag & Accessory Specials
CALL 800-588-9500 TO ORDER
Offers Expire Saturday January 22nd @ 2pm CST.
Standard Shipping Rates Only Apply to the 48 Continental United States.
This is a smoking hot deal on the PS90. For more info or if you have
questions go here www.fnforum.net:
http://www.fnforum.net
FN PS90USG 5.7X28mm Bull Pup Carbine Secret Service Sight
Black 16.04" Barrel 1-30rd Mag New
1-50rd Factory Mag & 4-50rd Asian Military Mags Free
#FN3848950260 $1197... 阅读全帖 |
|
|
w*******y
发帖数: 60932 | 6 Today kmart dumped a bunch of toys on clearance most are around 66% off
Good this we all have free shipping..right??
If you dont read this thread
http:// />
Kmart-Toys & Games: Star WarsSPEED STARS Lightsaber Launchers Slave 1:
http://iway.org/9636491
((Ship)) sale:2.5 old:9.99 dis:75%
Kmart-Toys & Games: UFC Basic Octagon Kmart Exclusive:
http://iway.org/9636501
((Ship|SPU)) sale:10 old:39.99 dis:75%
Kmart-Toys & Games: Star WarsSPEED STARSLightsaber Launchers V-Wing
Starfighter:
http://iwa... 阅读全帖 |
|
c****s
发帖数: 5892 | 7 加拿大两位政府官员称，通用汽车加拿大公司已经偿还了政府所有贷款。
综合外电4月21日报道，加拿大政府21日称，通用汽车加拿大公司已经偿还了经济衰退时期政府提供的全部贷款。
加拿大工业部长克莱蒙特(Tony Clement)和安大略省经济贸易和发展部部长Sandra PUP[综述 图片 论坛]atello在一个声明中发表上述言论。联邦政府和安大略省政府09年增加了对通用汽车加拿大公司的贷款。两级政府仍然拥有通用汽车的普通股和优先股。
克莱蒙特称，“加拿大政府和安大略省政府09年采取谨慎步骤，确保北美汽车工业仍然存在并可持续发展。通用汽车加拿大公司正在增加其奥沙瓦和英格索尔工厂的产量的事实，以及近期其他汽车公司的积极公告，均显示加拿大汽车工业正开始恢复势头。” |
|
X*******8
发帖数: 3895 | 8 刚才看有人在“请教一个转基因问题”，故把下面一篇文章转过来，没有翻译，有兴趣
的自己看吧。
http://www.holistichealthsecrets.com/alternativehealth/genetica
modified-foods-healthy-to-eat/
Are Genetically Modified Foods Healthy to Eat?
July 14th, 2009adminLeave a commentGo to comments
The topic of Genetically Modified Food (GMOs) can be confusing. On one side
we have big Pharma
companies touting studies that show that Genetically Modified Food is
perfectly safe for consumption. On
the other hand, we have wholistic health groups and speakers s... 阅读全帖 |
|
X*******8
发帖数: 3895 | 9 读读这个吧，没有翻译，有兴趣
的自己看吧。AAEM的人你不因该叫民科了吧？另外，谁把我关于先锋分类的贴删了？每
必要这么害怕吧？
http://www.holistichealthsecrets.com/alternativehealth/genetica
modified-foods-healthy-to-eat/
Are Genetically Modified Foods Healthy to Eat?
July 14th, 2009adminLeave a commentGo to comments
The topic of Genetically Modified Food (GMOs) can be confusing. On one side
we have big Pharma
companies touting studies that show that Genetically Modified Food is
perfectly safe for consumption. On
the other hand, we have wholistic health gr... 阅读全帖 |
|
|
b*******s
发帖数: 470 | 11 目前主流科学界的意见是：“狗是“天生”出来给人当好伙伴的”。今年四月份《
Science》有一期专门讲狗的自然驯化的专题：
Most experts now think dogs domesticated themselves. Early humans left piles
of discarded carcasses at the edges of their campsites—a veritable feast,
the thinking goes, for wolves that dared get close to people. Those wolves
survived longer and produced more pups—a process that, generation by
generation, yielded ever-bolder animals, until finally a wolf was eating out
of a person's hand. Once our ancestors realized the utility of the... 阅读全帖 |
|
M*******n
发帖数: 10087 | 12 他要不就是强词夺理，胡搅蛮缠
要不就是脑子坏了，一点常识都没有。
你Google “dog eat poop”，第一个link点进去，连老外都承认：
Your Dog Was Born to Eat Poop!
Before dogs were domesticated they were scavengers, living off of whatever
they could find. Dogs commonly fed on the waste of other animals (and other
dogs) thousands of years ago. Poop eating may just be a remnant of dog
history.
In certain situations, as with a newborn litter of puppies, eating poop is
instinctual and completely normal. A mother with pups is wired to keep her
den clean so ... 阅读全帖 |
|
c***l
发帖数: 2490 | 13
没觉得牛津大学的GTM丛书比Springer的GTM丛书B格高。PUP出的好的书都是那些鹅毛犹
太人写的。很多书都很薄，很薄。需要功底。
总之看作者不看出版社。看出版社太“那义务（江蛤语）”了。
一般鹅毛犹太人写的数学书都不错。鹅毛犹太人是美国数学学会一个很大的势力，经常
“打压中国人（星宿老仙语）”。
下面是牛津的GTM list
1. Keith Hannabuss: An Introduction to Quantum Theory
2. Reinhold Meise and Dietmar Vogt: Introduction to Functional Analysis
3. James G. Oxley: Matroid Theory
4. N. J. Hitchin, G. B. Segal, and R. S. Ward: Integrable Systems: Twistors,
Loop Groups, and Riemann Surfaces
5. Wulf Rossmann: Lie Groups: An Introduction through Linear... 阅读全帖 |
|
发帖数: 1 | 14 Yarmouth Port, Massachusetts
From Wikipedia, the free encyclopedia
Yarmouth Port, Massachusetts
Census-designated place
Boardwalk at Bass Hole
Location in Barnstable County and the state ofMassachusetts.
Coordinates: 41°42′17″N 70°13′15″WCoordinates: 41°42′17″N 70°13′
15″W
Country United States
State Massachusetts
County Barnstable
Town Yarmouth
Area
• Total 6.47 sq mi (16.76 km2)
• Land 6.04 sq mi (15.65 km2)
• Water 0.43 sq mi (1.11 km2)
Elevation 52 f... 阅读全帖 |
|
发帖数: 1 | 15 在网上发现了一个圣诞节商店，商店的建筑很有特色，而且遍布全美国，大概有70多个
店铺，以后大家买圣诞节用品可以去那里，似乎各州都有。我们这里和麻州的地址是：
CHRISTMAS TREE SHOP AUGUSTA, GA, 239 ROBERT C. DANIEL JR. PARKWAY,
AUGUSTA EXCHANGE, AUGUSTA, GA 30909, 706.738.0570, CHRISTMAS TREE SHOP
, MAGNOLIA PARK, 1117 WOODRUFF ROAD, GREENVILLE, SC 29607. 864. 297.
3667
CHRISTMAS TREE SHOPS NATICK, 1298 WORCESTER ST. ROUTE 9, SHERWOOD
PLAZA, NATICK, MA 01760, 508.655.9800
CHRISTMAS TREE SHOPS SOMERVILLE, 177 MIDDLESEX AVENUE, ASSEMBLY SQUARE
... 阅读全帖 |
|
发帖数: 1 | 16 3000块，买辆二三手Saturn Astra，加够油，可以去抓性奴了 |
|
|
w******z
发帖数: 1872 | 18 我觉得各种狗里面德牧算最漂亮威风的之一了， 突出特点是智商高，脾气好，好训练
啊， 我养过Husky, 笨死了， 模样倒是超级可爱归可爱， 笨死了 |
|
|
发帖数: 1 | 20 现在的有证纯血德牧并不好。 近亲太厉害，品种退化了， 有遗传病。
可以买个别的品种牧羊犬，有的智力比德牧还高一点。 |
|
|
l*******1
发帖数: 16217 | 22 养金毛或者拉布拉多吧 聪明温顺又听话
大狼狗太吓人了 |
|
l*******1
发帖数: 16217 | 23 养金毛或者拉布拉多吧 聪明温顺又听话
大狼狗太吓人了 |
|
w********2
发帖数: 632 | 24 The Gnome 7 Lambda was a French designed, seven-cylinder, air-cooled rotary
aero engine that was produced under license in Britain and Germany. Powering
several World War I era aircraft types it was claimed to produce 80
horsepower (60 kW) from its capacity of 12 litres (720 cubic inches)
although recorded figures are lower.[1]
Just under 1,000 units were produced in Britain, the majority (967) by the
Daimler Company of Coventry. A 14-cylinder variant was known as the Gnome 14
Lambda-Lambda.
In ... 阅读全帖 |
|
O*******d
发帖数: 20343 | 25 英文造词很笨,公母和幼兽都要造新词。
Animal Male Female Young
Ass Jack Jenny Foal
Bear Boar Sow Cub
Cat Tom Queen Kitten
Cattle Bull Cow Calf
Chicken Rooster Hen Chick
Deer Buck Doe Fawn
Dog Dog Bitch Pup
Duck Drake Duck Duckling
Elephant Bull Cow Calf
Fox Dog Vixen Cub
Goose Gander Goose Gosling
Horse Stallion Mare Foal
Lion Lion Lioness Cub
Rabbit Buck Doe Bunny
Sheep Ram |
|
m******n
发帖数: 6327 | 26 泰晤士报报道：《自然》杂志最近一期把巴马和老马做封面，无意中把两条狗做了封底。封面上左边巴马黑，右边老马淋浴金色阳光，封底左边是一条满眼期待，却又有点嫩的黑狗，右边是条看了忍不住想在老马耳朵后挠痒痒的黄狗。不是搞笑，这是真的。
September 26, 2008
http://www.timesonline.co.uk/tol/news/world/us_and_americas/article4831798.ece
Science journal in doghouse after Obama and McCain upstaged by young pups Latest issue of Nature compares presidental candidates on science, technology - but has unfortunate advert on back cover
"We didn't know until the issue landed on our desks," pleads Nature. "It just goes to show th |
|
p****n
发帖数: 6 | 27 The son of William F. Buckley has decided—shock!—to vote for a Democrat.
Let me be the latest conservative/libertarian/whatever to leap onto the
Barack Obama bandwagon. It’s a good thing my dear old mum and pup are no
longer alive. They’d cut off my allowance.
Or would they? But let’s get that part out of the way. The only reason my
vote would be of any interest to anyone is that my last name happens to be
Buckley—a name I inherited. So in the event anyone notices or cares, the
headline will be: |
|
l****z
发帖数: 29846 | 28 The Pentagon confirmed on Thursday the secret deployment in Afghanistan over
the past several weeks of the XM25, the newest advancement in small arms.
The XM25 is a revolutionary new Individual Airburst Weapon (IAW) system that
will change the face of war, much like the longbow, gunpowder and nuclear
weapons.
The XM25 is a revolutionary next generation of grenade launchers. Unlike the
single-shot M-79 "Bloop Tube" of Vietnam era, or the M203 tube slung under
M4 rifles, the XM25 is a semi-automat... 阅读全帖 |
|
l****z
发帖数: 29846 | 29 March 21, 2012 Posted by Shawn Mallow
By now, most all of us who follow politics have heard the collective barking
over Mitt Romney and his dog, Seamus.
Seems in 1983, Mitt saw fit to attach Seamus’ kennel to the top of his car
while driving to a family vacation.
I adore animals, including our clunky, slobbering, faithful doggies. And
while I can’t understand why anyone would pop a pup on the roof of their
car, the way this tail (excuse me, tale) has played out in the current
political realm i... 阅读全帖 |
|
|
l****z
发帖数: 29846 | 31 澳洲联邦参议院，7月17日投票表决，以39：32通过废除碳税法案。
澳洲联邦参议院在经过几天的辩论与商讨后，终于在本星期四的投票中，以39票赞成32
票反对的投票结果，废除了上届工党政府执政时推出的碳税法案。当澳洲参议院宣布这
一结果时，支持者们报以掌声。该法案获得通过，使澳洲成为世界上第一个废除碳税的
国家。
据天空新闻报导，澳洲联邦政府在本次投票前，成功地争取到了帕尔默联合党（PUP）
几位参议员，以及其他中立参议员的支持。在投票前，联邦政府与帕尔默联合党领袖帕
尔默（Clive Palmer）进行了长时间的谈判。
在上星期参议院的投票中，帕尔默在最后时刻撤回了对政府废除碳税议案的支持。帕尔
默当时认为，政府在处理过去达成的修正案的过程中，并没有采取认真合作的态度。不
过，自那之后，政府同意将采取更加严厉的措施，以保证废除碳税后，能源公司节省下
来的资金会转还给消费者。这使得帕尔默重新采取了支持政府的态度。
在投票中，帕尔默联合党的三名参议员以及来自澳洲汽车爱好者党（MEP）的参议员谬
尔（Ricky Muir）对废除碳税议案投了赞成票。
家庭第一党的戴伊（Bob Day）、自由民... 阅读全帖 |
|
t******r
发帖数: 8600 | 32 MeGrimlock4 26 points 2 years ago - (Around May 2015) - KEY DATE
I'm ready humans.
'Night gathers, and now my watch begins. It shall not end until my death. I
shall take no wife, hold no lands, father no pups. I shall wear no crowns
and win no glory. I shall live and die at my post. I am the sword in the
darkness. I am the watcher on the walls. I am the shield that guards the
realms of men. I pledge my life and honor to the Night's Watch, for this
night and all the nights to come.'
真正的美国英雄！ |
|
t**********c
发帖数: 4 | 33 大家好！Technomax是一家位于princeton junction的咨询公司，我们提供java，pup,
j2ee的免费培训和就业，下一个培训班将于7月25日开课，感兴趣的同学请联系 Emily.
E***[email protected] |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
