r******8
发帖数: 110 | 1 Batch purification的GST-tagged protein,要用Precission protenase切下GST,但GE
healthsciences的很贵,500 units 要大约$300,用不了几次,而且以后总要用.不知哪个
公司有便宜一点的卖? 谢谢! |
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r******8
发帖数: 110 | 3 是想自己在E.Coli表达纯化,但哪儿能弄到质粒? |
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y*********u
发帖数: 183 | 4 What I could think of: you can run a westernblot using CHIP samples before
protenase K treatment(1/10-1/20 depend on the expression level of your
protein of interest). If you pulled down a lot of your protein of interest,
and if your procedure is clean, I see no reason the CHIP-Seq wouldn't work
if your protein is indeed a DNA binding protein.
In worst case scenario you should still be able to get lots of strong peaks
which is particularly useful to you since your problem is that you didn't
have... 阅读全帖 |
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