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全部话题 - 话题: propidium
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M****e
发帖数: 70
1
来自主题: Biology版 - Re: 请大家帮忙
TUNEL labeling of tissue slides would tell you roughly how many apoptotic
cells are present on the slides. or you can try Hoechst staining for DNA
fragmentation. apoptotic cells are stained more bright for Hoechst, which is
thought due to change of chromatin structure. this is usually done with
propidium iodide staining, which stains dead cells bright. so apoptotic cell
is propidium iodide dim, and Hoechst bright. membrane structure change occurs
during apoptosis, and annexin V binds specificall
c*******0
发帖数: 190
2
Propidium iodide
535激发,很亮的红色...染出来很漂亮
k***g
发帖数: 4904
3
用了个cell membrane impermeable的染料染核酸,染formalin固定的细菌,染料名字
叫propidium iodide,manual上面说的不清楚,没看出来到底是常温还是37度下使用,
还号称incubate15分钟就可以了。请问一般的类似染料是啥温度下使用?如果37度下放
时间长了会不会失去荧光啊。
y***k
发帖数: 60
4
propidium iodide就是PI,我没染过菌,只染过细胞测细胞周期,室温5分钟就够了
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