df 发帖数: 319 | 1 我以前一直是 poly在 main,multifilament在cross。 |
|
w******n 发帖数: 13172 | 2 刚试过,打了两次就剪了。。。基本就是在打multi,结果既没multi的feel,又没有
poly的抓球 |
|
df 发帖数: 319 | 3 I found it that it gave me easier power however not going out, I could brush
the shorter balls up over the net with fewer going under. It's harder to
get the first flat serves in at least at the beginning. I felt better
placement making and improved plow through on my single hand backhand.
Maybe it was just that the new strings work better. I tended to leave the
poly mains too long until the cross multifilament broke, when the mains were
long time over due. |
|
w******n 发帖数: 13172 | 4 你用全副软poly,上低3-5磅就什么问题都解决了,既有power,又抓球不出界,还经打
。这么搞performance没什么问题,唯一可能的是有些人觉得比较伤胳膊,当然我从来
没觉得。
brush
were |
|
df 发帖数: 319 | 5 Any recommendation on soft poly? I have been using luxilon alu power rough
for years on the main. |
|
M*****n 发帖数: 282 | 6 那完了,你换什么都不行了。Once Alu, always Alu.
[在 df (unkown space) 的大作中提到:]
:Any recommendation on soft poly? I have been using luxilon alu power rough
:for years on the main.
:........... |
|
发帖数: 1 | 7 现在用的一种便宜的 Poly打几天走线就很严重了 |
|
f****l 发帖数: 27 | 8 http://www.crfg-central.org/calendar.htm
看着还不错,想去去观摩一下
Mark your calendars -- this meeting will be on the third Saturday -- for our
annual Grafting Event and Scion Exchange. This has been the most heavily
attended meeting of past years! Plan on coming early for grafting lessons
and the scion exchange. There will be grafting supplies for sale.
Date: Saturday, Feb. 21, 2015
Time: 1:30 p.m. until 3:30 p.m.
Location: Crops Unit at Cal Poly- Corner of Highland and Mt. Bishop Rd.
Cost: Free
Who: Open ... 阅读全帖 |
|
r********3 发帖数: 2998 | 9 毫无疑问是NYU。
我记得前几年就已经有人在Gter家园上爆poly那个学校很扯了。没想到现在还是这样扯
。 |
|
c********g 发帖数: 449 | 10 Cal poly. ,simply. The best. |
|
m******g 发帖数: 621 | 11 你这两个学校MS去别的地方读PHD难度比较大吧。
如果MS直接找工作,我还是推荐SCU。
CAL POLY你是哪个?POMONA还是SAN LUISPO。
一句话,位置最重要,最好不要离开弯曲 |
|
g***m 发帖数: 465 | 12 其实没必要控制cycle,虽然书上这么建议的。
俺先后用过两个proofreading,先是用NEB的Deep Vent,这个酶的忠实度狠好,俺用来构
建质粒,35cycles,将近1k的三个片段拿去测序,没有错误。
后来作RT PCR的时候因为template是total poly A RNA,而Deep Vent没有Hot start,结
果不是太consistant,有时候有,有时候没有,产率也偏低。俺就换到Pfx plantium
(Invitrogen),号称市面上忠实度最高的,现在所有RT PCR都作出来了,35cycles,3K的
片段。先拿了一个基因的三个克隆测序,结果是在两端大致1.5k的长度上DNA序列有2-4个
错误,氨基酸序列没有错误。而且由于俺同时测了三个克隆,以后可以用没有错误的克隆
拼接成error free的一个全长片段。
总的来说,Mg的浓度不要太高,酶的用量要尽可能少,然后就是wait and pray了,呵和
。
好运! |
|
j****x 发帖数: 1704 | 13 想要确保获得有效的RNA Polyadenylation,后一个可能是不够的。
狭义上的polyA signal,指的是RNA序列上的一个Conserved motif: AATAAA(AAUAAA)
。CPSF蛋白识别并结合该位点,在CstF的辅助下切割下游pre-mRNA,再由PAP完成加尾
。当然整个过程还需要其他一系列蛋白因子的共同左右,不细说了。
你的问题涉及到CstF这个关键因子,它识别并结合AATAAA下游一段GU rich region,对
于有效的RNA Polyadenylation processing是不可或缺的,尽管这段序列不像poly
signal motif那样具有比较严格的consensus sequence。而在你的第二个版本中,并没
有包含这段序列。 |
|
m******5 发帖数: 1383 | 14 非常感谢!
还有一个问题,挺多人设计knock in allelle,往往并未提及如何添加poly A
sequence,在这种情况下是什么原因 |
|
m******5 发帖数: 1383 | 15 非常感谢! 主要是我设计了一个漏掉了GU rich sequence的poly A 在我的knock in
sequence的尾端,现在已经拿到老鼠了,刚意识到这个问题。
现在不知道严重程度,因为还没有检测基因的表达 |
|
H*********8 发帖数: 323 | 16 搭车问个问题,我在某个基因内部插入cre 基因,cre基因的终止子后面就没放poly a
,转录出来的mRNA是融合的,翻译成蛋白的时候能不能在cre 终止子处终止翻译呢?
谢谢! |
|
H*********8 发帖数: 323 | 17 如果是在in frame reading,我就想知道,如果没有poly a,能不能够在终止密码子处
停止翻译。 不要说理论的,我想理论上是可以的。我想知道有没有人有这方面的经历。 |
|
f******9 发帖数: 22 | 18 Does anyone know if there is any different between human growth hormone
polyA and bovine growth hormone polyA? why some expression cassette use
human other use bovine? Any specific purpose?
Plus bovine is much short than human ones, make me wonder why we do not use
bovine poly instead.
Anyone knows? Thanks. |
|
h******0 发帖数: 150 | 19 帮朋友请教一下,这几个学校都没有奖学金,请问选哪一个比较好,
1. University of California, Santa Barbara (UCSB)
MA in Economics with an emphasis in Business Economics, one year
program
2. Case Western Reserve University (CASE), MBA, two-year program
3 NYU-Poly, MS in Management, two-year program
4 Ceibs 中欧, MBA
请提供一些信息,多谢啦! |
|
s***h 发帖数: 56 | 20 【 以下文字转载自 Macromolecules 讨论区 】
发信人: shish (shish), 信区: Macromolecules
标 题: 包子求篇上周ACS POLY的preprint, thanks
发信站: BBS 未名空间站 (Mon Mar 29 13:58:28 2010, 美东)
Surface active zwitterionic block copolymer synthesis for antifouling
applications and surface characterization.
Sundaram, Harihara S.; Cho,
Youngjin; Weinman, Craig J.; Paik, Marvin Y.; Dimitriou, Michael D.;
Finlay, John; Callow, Maureen E.; Callow, James A.; Kramer, Edward J.;
Ober, Christopher K. Department of Materials Science & Engineerin |
|
c******y 发帖数: 193 | 21 自己顶一下。
有ACS POLY MEMBERSHIP的同学只需要进下面这个链接,输入
ID:0ACS NUMBER
PASSWORD:POLYMER
http://www.polyacs.org/11.html this has instruction, use you ID number start with 0, and password as 'polymer'.
非常感谢! |
|
d********s 发帖数: 23 | 22 发信人: dendrimers (xiaoqiu), 信区: Macromolecules
标 题: 审稿机会 方向是thermal transport capacity of Poly(3-hexylthiophene)
Films
发信站: BBS 未名空间站 (Mon Dec 17 21:39:46 2012, 美东)
有兴趣并且方向match的同学请站内和我联系一下,方便的话给我一个简单的CV |
|
h******0 发帖数: 150 | 23 帮朋友请教一下,这几个学校都没有奖学金,请问选哪一个比较好,
1. University of California, Santa Barbara (UCSB)
MA in Economics with an emphasis in Business Economics, one year
program
2. Case Western Reserve University (CASE), MBA, two-year program
3 NYU-Poly, MS in Management, two-year program
4 Ceibs 中欧, MBA
请提供点建议。 |
|
l*******s 发帖数: 36 | 24 一个纽约,一个波士顿(费用较贵),不好选~
打算偏硬些,VLSI.
感觉neu的专业水平更好。
poly吸引我的地方是打算如果以后有可能的话转到Master of finance engineering去
,不太想以后天天对着电脑写code.
希望大家畅所欲言,谢谢了。 |
|
w*******d 发帖数: 3714 | 25 NEU has somehow better location. But Poly would become NYU eventually, which
is a huge advantage! |
|
J*******d 发帖数: 72 | 26 for a-Si:H and uc-Si:H(or nc-Si:H), pin structure is commonly used. however,
for poly-Si, it seems no pin structure yet, only p-n diode structure. Why?
any super cow can give me a reasonable explanation?
Thanks! |
|
J*******d 发帖数: 72 | 27 I think it should called uc-Si instead of poly-Si, strictly speaking. Do you
agree? |
|
s*****v 发帖数: 145 | 28 【 以下文字转载自 JobHunting 讨论区 】
发信人: semenov (seme), 信区: JobHunting
标 题: 这里有没有Poly-tech的童子,我想核实一下就业情况
发信站: BBS 未名空间站 (Sun Mar 15 12:57:01 2009)
家有小表弟,拿了这个学校硕士的小奖,问我怎么样。我个人没有听说过这个学校,他
说这个学校是NYU的一部分。还给跟我说这个学校EE就业情况非常好,中国学生一半多
都找到工作了。
有没有童子说说到底怎么样??
我要是离得近就去看看这个学校了,无奈离得很远而且工作比较忙。提前谢谢大伙了!! |
|
|
a********e 发帖数: 3771 | 30 poly的EE只要交钱就能上,实在太垃圾了。他们的EE学生毕业以后基本上回国找工作。 |
|
s****o 发帖数: 62 | 31
poly 是不是交钱就能上我不知道 不过EE毕业后基本上回国找工作? 你搞笑吧? 我
就今年秋季毕业的 我认识的十几个EE同学 只有两,三个到现在还没工作
就凭你这说话跟放屁一样的水平 你才是垃圾
至于楼主说的TAMU 我不了解 就不发表意见了 |
|
e******z 发帖数: 1 | 32 Is there a database or a website that have those physical constants
of polymer like pKa, more specifically i would like to know the pKa
of PAA (Poly Acrylic Acid) 's pKa.
A specific handbook or paper is also ok. Thanks a lot! |
|
e*********s 发帖数: 1440 | 33 I have tried this. Unfortunately, it didn't work. The problem is that poly(
2-vinylpyridine) is coordinated with the copper salt. I did the synthesis
based on literature, which says the solution is colorless after passing
through alumina column. Maybe there is a tricky issue that I don't realize.
the |
|
D********g 发帖数: 533 | 34 hmmm... then the copper may be ligated via pyridyl groups... I also want to
know the tricks... since my polymer also can form ligation with copper and
after twice the column and it is still light green. However, isopropyl
alcohol definitely cannot get any ligands out, at least for my polymer.
Good luck.
poly( |
|
s***h 发帖数: 56 | 35 Surface active zwitterionic block copolymer synthesis for antifouling
applications and surface characterization.
Sundaram, Harihara S.; Cho,
Youngjin; Weinman, Craig J.; Paik, Marvin Y.; Dimitriou, Michael D.;
Finlay, John; Callow, Maureen E.; Callow, James A.; Kramer, Edward J.;
Ober, Christopher K. Department of Materials Science & Engineering,
Cornell University, Ithaca, NY, USA.
Abstracts of Papers, 239th ACS
National Meeting, San Francisco, CA, United States, March 21-25, 2010
(2010), POLY- |
|
c******y 发帖数: 193 | 36 Hi, I need to download a paper in Polymer Preprint, which could only be
downloaded by ACS POLY member.
Heparin-containing hydrogels for modulating endothelial cell responses.
Polymer Preprints 2008, 49(2), 584.
Thanks a lot! |
|
c******y 发帖数: 193 | 37 自己顶一下。
有ACS POLY MEMBERSHIP的同学只需要进下面这个链接,输入
ID:0ACS NUMBER
PASSWORD:POLYMER
http://www.polyacs.org/11.html this has instruction, use you ID number start with 0, and password as 'polymer'.
非常感谢! |
|
c*******n 发帖数: 82 | 38 请教各位poly(N-isopropyl acrylamide)的一些温敏性质:
如果我把这个高分子的水溶液和有机溶剂(hexane or iso-octane)混合,然后把溶液加
热到50度左右,它会从水相迁移到有机相吗?请熟悉这个高分子的高手解答。 |
|
u*****e 发帖数: 357 | 39 Polym. Prepr. 2008, 49(2), 483-484.
有ACS POLY MEMBERSHIP的同学只需要进下面这个链接,输入
ID:0ACS NUMBER
PASSWORD:POLYMER
http://www.polyacs.org/11.html this has instruction, use you ID number start with 0, and password as 'polymer'.
非常感谢! |
|
m****n 发帖数: 293 | 40 去TAMU吧, 尽管NYU POLY的EE还水平可以。
也别学什么nuclear engineering, 啥热门就学啥吧, 象CS什么的。
学nuclear engineering以后只能喝西北风了。
我就说这么多了, 再问我就要咨询费了。 |
|
D*****I 发帖数: 8268 | 41 TAMU吧,Poly校园小的要死。。
核工程就算了。。 |
|
|
s**a 发帖数: 8648 | 43 【 以下文字转载自 Sociology 讨论区 】
发信人: sasa (无空), 信区: Sociology
标 题: Research in Poli Soc Vol.17 Call for Papers
发信站: BBS 未名空间站 (Sun Apr 22 18:46:57 2007)
Research in Political Sociology is accepting manuscripts for Volume 17.
This volume will broadly focus on 'Politics and Public Policy.' The primary
objective of Research in Political Sociology is to publish high quality,
theoretical informed empirical research in areas that advance the
understanding of politics in society. Manuscripts submitted for |
|
b***k 发帖数: 2673 | 44 ☆─────────────────────────────────────☆
LilyBlossom (LilyBlossom) 于 (Fri May 16 01:35:07 2008) 提到:
poly tech MFE $7000 scholarship,值不值得去读?看上去学校位置很好,不知道这个
地利有多大作用。如果quant的背景不是十分好的话(只学过微积分和线代),本科读
finance.读这个对以后就业究竟有没有好处?
☆─────────────────────────────────────☆
LilyBlossom (LilyBlossom) 于 (Sun May 18 19:24:06 2008) 提到:
希望知情人可以交流一下,不胜感激!
☆─────────────────────────────────────☆
yuandt (TT) 于 (Sun May 18 21:44:45 2008) 提到:
☆─────────────────────────────────────☆
yuandt (TT) 于 (Mon |
|
z*y 发帖数: 77 | 45 Hi,
请问poly的master of science in financial engineering这个program,毕业好找工
作吗?这个版的名字金融工程,如果主题不符请帮我转载。谢谢! |
|
k*****a 发帖数: 7389 | 46 NYU-Poly的MSFE is better than CMU's |
|
s**********r 发帖数: 8153 | 47 what is Poly? u mean NYU? |
|
m*******e 发帖数: 6358 | 48 据fcinternews,波利正式黄了
So that now Poli thanked Inter and now feel free to negotiate with other
companies that are interested. According to Sky, these are Napoli, AC Milan
and Juventus also. |
|
|
|