l*****l
发帖数: 5909 | 1 作者：沈睿
我1月13号晚上写的阅读余秀华的诗歌的博文，突然goes viral ，点击超过五十万，据
说微信上都在转，成千上万的人说余秀华的诗歌怎样好，吓了我一跳，跟去年五月我的
博文《舌尖上的中国，有多好》吓我一跳差不多，不过后者让我吓一跳是给我带来的成
千上万的谩骂和辱骂。（http://www.douban.com/note/478467876/?src=noti）
好几个出版社杂志社找我，跟我要余秀华的地址电话。我答我不认识余秀华，跟她没联
系，不知道她住在哪里，只知道她是湖北人。我的儿子也写信问我：妈妈她是哪儿人？
湖北，我说。哪个县？我大笑，我哪里知道！我是网上知道她在湖北，我跟她没说过话
，我不认识她。
我只看诗不看人。我觉得余秀华的诗很棒，不在乎她是脑瘫还是脚瘫，就是她身体没受
到损伤，我也喜欢她的诗歌。她的不完美的身体，她的容貌，跟我欣赏喜欢她的诗歌没
有关系。我最烦“美女”这两字，一个写字的人不是电影演员，不靠脸蛋吃饭，一个诗
人不是国家主席夫人，靠穿衣服给中国争面子，美女怎样，不美女又怎样？余秀华诗写
得好，语言横空出世，与她的天分有关，与她的身体受限制有关，与... 阅读全帖 |
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s*****g
发帖数: 5159 | 2 start with 35lux a
then 50cron or 50 noti, depends on your skills and pocket. |
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b*s
发帖数: 82482 | 5 From: B********[email protected] (Bruce Grubb)
Here's Version 4.6.3 (Dec 1, 2000)
The Mac-FTP-list lists over 120 mac anonymous ftp sites (some with notes),
over 100 Mac web pages, and contains some instructions on how to use
anonymous
ftp and find files as well a format chart showing which programs decompress
and decode which files. Also there is a section on how to use certain
files.
Since the list is as much a web page list as a FTP one I have desided to
rename it the Mac-site-list and you will noti |
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j*****e
发帖数: 11116 | 6 你再看一遍吧 这个跟我说的不是一回事 这个是voicemail的noti |
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b******r
发帖数: 111 | 7 Good idea. My 9kb DNA itself contains too many restriction sites. NotI is
the only choice.
'Description:
Important: GeneHogs® competent cells are being discontinued on December
31, 2007.
Use ElectroMAX™ DH10B™ T1R cells for exceptional performance!
'
常做的克隆比是不是还比较正常，如
果比较正常，那说明这种假阳性很可能是因为你的插入片段有一段序列比较容易发生重
组，试着30度长克隆和摇菌看看。
另外，能不用单酶切做克隆就不要用，你大片段克隆还用这个策略，最好换其它双酶切。
这个菌株，从来没出现过问题，感
受态效率一般7次方就可以了，15kb可以长百来个克隆。
发生重组的质粒，一般10个有2个
总是对的。 |
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e**e
发帖数: 166 | 9 NotI GQ, 1,000u
what does GQ mean?
thanks |
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b******r
发帖数: 111 | 10 Hi,everyone,
I have to use single restriction sites for insertion of multiple fragments
. When using KpnI site, just get one successful insertion from 70 clones.
For NotI, it is 1 from 4,BamHI is 1 from 2. My question is why the ligation
is so bad at KpnI site? Or other reasons? Thanks |
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D*a
发帖数: 6830 | 11 正常的PCR错配比例很低而且每个碱基的几率是一样的，所以错配的只是测序里面的背
景噪音，对测序结果没有任何影响。只有在需要单个分子的时候，比如做分子克隆或者
做nested PCR时，才要考虑到单个的分子可能有突变。nested PCR解决这个问题的方法
就是不要大量稀释，而是降低循环数（提高模版来源于不同PCR产物的数量，同时降低
了PCR反应的次数所以也减少了错配的次数）
sequence
region
NotI
吗, |
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j**W
发帖数: 89 | 12 You are right about a couple of things.I switch field and learned biology (
not including molecular biology) in English.And I did not make the targeting
vector. I took over the project starting from agouti mouse. Please bear
with me if I could not be clearer. I am sorry about the confusion on the
primer used for sequencing. We did sequencing with both P1 and P2. One came
back with no good sequence at all. From the sequencing result of the other,
it seems to me like P1. The sequencing result cove... 阅读全帖 |
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n****3
发帖数: 543 | 13 你要对载体去磷酸化，我克隆了3kb到27kb的载体内通过notI, 挑了5个有3个是， 测
序鉴定没有问题 |
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n****3
发帖数: 543 | 14 你要对载体去磷酸化，我克隆了3kb到27kb的载体内通过notI, 挑了5个有3个是， 测
序鉴定没有问题 |
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w*****t
发帖数: 179 | 15 为什么连不进去呀，我连过很多cdna,都成功了呀，我用的是agilent 的系统，如果你
担心ITR有突变的话，可以先在PCMV-MCS中连，在用NOTI切后连到PAAV-MCS中去，之后
转到重组酶缺失的大肠杆菌中，如XL10-GOLDEN 或者SURE中。现在我用INFUSION
CLONING几乎都是阳性。 |
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m**i
发帖数: 84 | 16 文成武德,千秋万代,一统江湖
zz
发信人: crazyman (企予望之，步立踯躅), 信区: Immigration
标 题: 我的EB1A 140 (TSC PP) 也approve了
发信站: BBS 未名空间站 (Mon Apr 9 20:15:02 2012, 美东)
30号寄出的， 刚刚收到email
On April 9, 2012, we mailed you a notice that we have approved this I140
IMM
IGRANT PETITION FOR ALIEN WORKER. Please follow any instructions on the
noti
ce. If you move before you receive the notice, call customer service at 1-
80
0-375-5283. |
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b****n
发帖数: 35 | 17 Thanks for both of ur reply. Sorry I forget to follow up. It sounds these two
markets r very dynamic and complicated, just like chicken-and-egg things.
Actually I am more interested in the local currency (S$) against US$. I noti
ce S$ slides whenever the Singapore government-linked-corporation acquires
some big oversea companies. The selling of S$ in exchange of US$ for the acquis
ition pushes the depreciation. But S$ recently rebounds back due to the weak
ness of US$.
I have a hunch that S$ has |
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j****x
发帖数: 943 | 18 Been a PhD student for 5 years now and never heard of it. I think this
probably because the Chicago Consulate sucks, they never bother to noti
fy me or anyone that I heard know (maybe the Chinese student association
never notify us at all). Nonetheless, the application material is
interesting.
6．研究课题报告或课题介绍（中文，不少于2000字）；
7．曾发表的主要学术论文、著作、作品的封面、目录、首页等证明材料及1000字以内
的中文摘要；
8．国际学术会议邀请函或相关证明。 |
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b*******s
发帖数: 954 | 19 Thanks! It is like a personal message left online. Anyone who follows
can see your message. You can see the messenger left by anyone you
follow. Therefore, if we have a group, a group member can send out a
piece of message; all the other group members will see this message. It
save everyone's time since you don't need to send a similar message to
different person. Instead, you send a message once, everyone who is
interested in seeing it will know...
I'm testing drive it now and i like it. I noti |
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q***z
发帖数: 543 | 20 对阿我也选4但是是错的
Correct Answer: 2 Your Answer: 4
RATIONALES: Headache is common after a head injury;
however, it may also be a sign of worsening neurologic
condition. Therefore, the nurse should administer
acetaminophen to try to manage the client's pain without
causing sedation. The nurse should then reassess the
client in 30 minutes to note the effectiveness of the pain
medication. Although a headache is expected, the client
should receive treatment to alleviate pain. The physician
should be noti |
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s*********s
发帖数: 1205 | 22 A majority of Americans are unhappy with their jobs, according to a recent
Conference Board survey. What does this mean? For one thing, it’s a clue
that as soon as this economy improves, an awful lot of people are going to
be setting off for greener pastures.
Now is a good time to talk about how to quit a job with class. (A lot of
this also applies to how to leave a job classily under any circumstances,
voluntary or not.)
It helps to break down the process into three phases: before you give noti |
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h*******e
发帖数: 5550 | 23 小片在偶的FACEBOOK上玩游戏，跟一堆人发出NOTI。。。 |
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