V***b
发帖数: 3419 | 1 The reason that rapamycin inhibits mTorc2 is that long-term treatment with
rapamycin, overnight for example (concentration doesn't matter), inhibits
mTorc2 complex assembly, but rather inhibits its kinase activity directly. |
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l*****7
发帖数: 8463 | 2 Cancer researcher retracts 19 studies at once
with 23 comments
jcheng
Jin Cheng
A former cancer biologist at the Moffitt Cancer Center in Tampa, Florida has
retracted 19 papers from a single journal.
Jin Cheng, who studies how ovarian cancer develops, withdrew 19 papers from
the Journal of Biological Chemistry originally published over the last 15
years, and corrected another. All of the retractions are for image
manipulation.
For example, here’s the notice for “Activation of phosphatidylinosito... 阅读全帖 |
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j********r
发帖数: 156 | 3 Generally, the kinases for T308 and S473 of Akt are PDK1 and mTOR complex 2
respectively. PI3K causes the production of PIP3 on the membrane, which then
binds to both Akt and PDK1 (which is assumed to be constitutively active).
So far there seems no direct evidence as to how mTORC2 is regulated. However
, inhibition of PI3K in most cases will lead to the suppression of
phosphorylation at S473.
Just curious, what is the evidence for PI3K-independent S473 phosphorylation
in your experiment? PI3K h... 阅读全帖 |
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s********s
发帖数: 67 | 4 高浓度的rapamycin抑制mtorc2原因搞清楚了吗？谢谢! |
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l**********1
发帖数: 5204 | 5 >被揪出来后，还有很多人居然好意思继续在圈子里混。
Just changed one and next one lab then it is ok for next career possible.
Pls refer:
MTORC1 and mTORC2 differentially regulate homeostasis of neoplastic and non-
neoplastic human mast cells
Authors: Daniel Smrz, Mi-Sun Kim, Shuling Zhang, Beverly A Mock, Sárka
Smrzová, Wendy Dubois, Olga Simakova, Irina Maric, Todd M Wilson, Dean D
Metcalfe, Alasdair M Gilfillan
Blood. 11/2011;
Cytokine Production and Chemotaxis in Human Mast Cells
Authors: Madeleine Rådinger, Hye ... 阅读全帖 |
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