x*****o
发帖数: 441 | 1 严格来说,single molecule分很多大方向.Chu,ha还有小薇主要是single molecule
FRET, tweezer做得不多 ,不涉及拉扯.主要研究平衡或非平衡态下得分子自发形变.
Bustamante,block主要是optical或者megnet tweezer,荧光涉及比较少,主要是生物分
子受力下的机理.
这两拨人做得东西相差甚远,但都涉及生物过程的动力学. 但是分析数据的方法不太相
同.
其他的single molecule方向还有sunny xie的活细胞confocal,小薇他们在做的super
resolution等等等等
要是想跳最好找个好老板,这行比较看血统,都是嫡系把持的.好老板还是有很多能做的
好方向.还有就是想清楚是想做哪种single molecule,分类太多了. |
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x*****o
发帖数: 441 | 2 这个矛盾是一直有的.
就像我以前的老板,是理工背景的,有single molecule仪器.然后找生物牛人,什么HHMI
的合作,paper可以发的很好. TJ ha也是走这个路子的.
当然我们也有自己的生物系统,就比较难搞,你不是那个圈子里面的,想要做那个,是很难
进人家那个圈子的.
新技术是single molecule人的骄傲. 我老板不管如何巴结大牛发自己系统的paper或者
拉项目,我们实验室发展的新技术都是亲生的.这个很难,以前是一个科大很聪明的男生
在做.
我的建议是: 纯生物背景如果是PI,想做single molecule,就找个人合作,送样品过去,
那边给作了,一起发paper.自己做是不可能的.如果是做postdoc,想学学single
molecule,可以找个好点的组,呆上几年,慢慢学,不必被门槛吓到了.以后出来做faculty
不就两个背景都有了. |
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Y********6
发帖数: 51 | 3
We worked on all the techniques in the single molecule field. We are
currently looking for people to build optical tweezer, combination of
optical tweezer and single molecule FRET, magnetic tweezer and the
combination of magnetic tweezer and FRET, AFM, patch clamp and the
combination of patch clamp with single molecule fluoresnce or CARS, super
resolution microscopy including PALM, STORM, SIM, spinning disc, bessel beam
microscopy, Inverted selective plane illumination microscopy, etc.
At the b... 阅读全帖 |
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s*****l
发帖数: 1844 | 4 【 以下文字转载自 Pharmaceutical 讨论区 】
发信人: seagull (bigfish), 信区: Pharmaceutical
标 题: Small Molecules Layoff Crazy from Pfizer
发信站: BBS 未名空间站 (Tue Dec 1 16:09:11 2009, 美东)
Just hear Pfizer is layoff all small molecules staffs in its St Louis site (
~600 people), close the Collegeville site from Wyeth (mostly small molecule)
, layoff all small molecules people at Pearl River and Princeton sites from
Wyeth......
Overall 22 R&D sites reduced to 9. These positions may be gone for ever
with the outsourci |
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m******i
发帖数: 834 | 5 观看碳纳米管(内分子)形状的变化，Vision molecules change shape inside
nanotubes
【纳米科技世界快讯】High-resolution images of shape changes in retinal – a
molecule important for vision – may help us to understand how we see,
report researchers in Japan. Using a transmission electron microscope (TEM),
Kazu Suenaga and colleagues at the National Institute of Advanced
Industrial Science and Technology (AIST) imaged a single retinal molecule by
attaching it to a carbon-60 molecule trapped inside a carbon nanotube. The
work m |
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Y********6
发帖数: 51 | 6 Dear all:
We just started a new company about single molecule instrument (Suzhou
single molecule instrument development Co. Ltd) and we want to hire people
with background in one of the following field: optics, microscopy, single
molecule biophysics, biophotonics, nanophotonics, electrophysiology, optical
tweezer, magnetic tweezer, AFM, etc. If you have experience in the
instrument design or have a Ph.D degree in the related field, we would like
to invite you to join us in either full or part ti... 阅读全帖 |
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x*****o
发帖数: 441 | 7 呵呵,如果就是postdoc其实也没那么高的要求了.当然对自己要求高点是对的. single
molecule主要是拼仪器,要是实验室仪器已经有了,实验上手也不是很复杂.新来的阿三
小弟小妹就是一个月就独立做试验了.
当然仪器都没有就不要想了,搭仪器要专业的. 物理的方面的要求主要是搭仪器和数据
分析.要是要求不高,传统的数据分析是很成熟的套路,到专业老板那里学一两年就行了.
要是想通过single molecule data构建复杂的模型就得有点底子.建议看看小薇有的
single molecule paper,数理方程,公式一堆一堆的,砸死你.不过我科大的同事说这个
其实没什么必要, Ha没有这些照样发CNS.
要是想跳super resolution, imaging这方面最好有编程基础,这些主要靠这个.
说道这个技术的局限性,是很大的. 最大的局限就是在数据分析里面的主观性. 数据分
析的时候,都是靠人为选取好得单分子和删除噪音.这里面就有很多主观得因素了.以致
于组里面的mm说觉得自己每次分析数据都是在cheating.........
如果想在这行做PI,一直做这个,还是不... 阅读全帖 |
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Y********6
发帖数: 51 | 8 Dear all:
We just started a new company about single molecule instrument (Suzhou
single molecule instrument development Co. Ltd) and we want to hire people
with background in one of the following field: optics, microscopy, single
molecule biophysics, biophotonics, nanophotonics, electrophysiology, optical
tweezer, magnetic tweezer, AFM, etc. If you have experience in the
instrument design or have a Ph.D degree in the related field, we would like
to invite you to join us in either full or part ti... 阅读全帖 |
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Y********6
发帖数: 51 | 9 Dear all:
We just started a new company about single molecule instrument (Suzhou
single molecule instrument development Co. Ltd) and we want to hire people
with background in one of the following field: optics, microscopy, single
molecule biophysics, biophotonics, nanophotonics, electrophysiology, optical
tweezer, magnetic tweezer, AFM, etc. If you have experience in the
instrument design or have a Ph.D degree in the related field, we would like
to invite you to join us in either full or part ti... 阅读全帖 |
|
Y********6
发帖数: 51 | 10 Dear all:
We just started a new company about single molecule instrument (Suzhou
single molecule instrument development Co. Ltd) and we want to hire people
with background in one of the following field: optics, microscopy, single
molecule biophysics, biophotonics, nanophotonics, electrophysiology, optical
tweezer, magnetic tweezer, AFM, etc. If you have experience in the
instrument design or have a Ph.D degree in the related field, we would like
to invite you to join us in either full or part ti... 阅读全帖 |
|
s*****l
发帖数: 1844 | 11 Just hear Pfizer is layoff all small molecules staffs in its St Louis site (
~600 people), close the Collegeville site from Wyeth (mostly small molecule)
, layoff all small molecules people at Pearl River and Princeton sites from
Wyeth......
Overall 22 R&D sites reduced to 9. These positions may be gone for ever
with the outsourcing going on, just like the auto industry.... |
|
Y********6
发帖数: 51 | 12 Dear all:
We just started a new company about single molecule instrument (Suzhou
single molecule instrument development Co. Ltd) and we want to hire people
with background in one of the following field: optics, microscopy, single
molecule biophysics, biophotonics, nanophotonics, electrophysiology, optical
tweezer, magnetic tweezer, AFM, etc. If you have experience in the
instrument design or have a Ph.D degree in the related field, we would like
to invite you to join us in either full or part ti... 阅读全帖 |
|
Y********6
发帖数: 51 | 13 Dear all:
We just started a new company about single molecule instrument (Suzhou
single molecule instrument development Co. Ltd) and we want to hire people
with background in one of the following field: optics, microscopy, single
molecule biophysics, biophotonics, nanophotonics, electrophysiology, optical
tweezer, magnetic tweezer, AFM, etc. If you have experience in the
instrument design or have a Ph.D degree in the related field, we would like
to invite you to join us in either full or part ti... 阅读全帖 |
|
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p*l
发帖数: 1359 | 15 PALM STORM这一类的super resolution技术，是基于单分子成像，图像合成就是一个个
单分子数出来。所以算single molecule技术也没说错。
molecule? |
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s****n
发帖数: 234 | 16 Assuming you have done three independent data collections, each of which
contains dataset from 5000 molecules, I would analyze the data in two
different ways:
(1) calculate the statistics (mean velocity, mean FRET efficiency or ...)
for each 5000-molecule dataset, calculate the standard deviation of the mean
of the three statistical values (SEM), and present the data as
+ SEM;
Or
(2) pool all the data from three data collections and calculate the mean
statistics +/- Standard Error o... 阅读全帖 |
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f******g
发帖数: 1003 | 17 想做个small molecule screening，但是自己又不是这个方面出身的，请问国内有做
small molecule screening 比较好的公司吗?
费用如何?
thanks |
|
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O*******f
发帖数: 926 | 19 【 以下文字转载自 Pharmaceutical 讨论区 】
发信人: Organoelf (King), 信区: Pharmaceutical
标 题: Scientist/Assocaite Scientist in Small Molecule roles
发信站: BBS 未名空间站 (Fri May 27 02:09:46 2016, 美东)
a recruiter at LinkedIn contacted me this Monday to see if I have friend(s)
who would be interested with this position. I just saw his message today.
Sorry, but any details in my hand yet. It seems focusing on small molecule
synthesis, I guess.
I can show you the recruiter's name at LinkedIn, and you may contact him
dir... 阅读全帖 |
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t********i
发帖数: 10 | 20 【 以下文字转载自 Chemistry 讨论区,原文如下 】
发信人: tetsuhashi (lulu), 信区: Chemistry
标 题: What software can calculate the IR transition frequency of a molecule? Matlab? Chemoffice?HyperChem?
发信站: The unknown SPACE (Fri Jun 28 05:25:04 2002) WWW-POST
Could anybody recommand a software that can calculate the IR transition
frequency (IR spectrum) of a molecule and assign the frequencies to modes?
Esp. excited state IR abs. Matlab? Chemoffice? HyperChem? I learnt from
literature that Gaussian98 can. But where to g |
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w******y
发帖数: 8040 | 21 so what's wrong with small molecules???
(
molecule)
from |
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l******t
发帖数: 2243 | 22 so everybody touches small molecules in those sites get laid off? discovery,
DMPK, formulation...?
(
molecule)
from |
|
发帖数: 1 | 23 The Molecular Informatics department performs molecular design and
informatics support to the small molecules and biologics drug discovery
programs within the R & D center at Shanghai Hengrui Pharmaceutical Inc.
Opening position
Scientist/Senior scientist:
We have an open position for small molecule drug discovery and scientific
computing. This person will actively provide computational support for
various drug discovery projects at different stages independently or under
limited supervision. In... 阅读全帖 |
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w*******o
发帖数: 739 | 25 如题：Molecules 需要审稿人两枚，请审过这个稿子的站内信联系，谢谢。
(Before Oct 1st) |
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a****k
发帖数: 117 | 26 需要三个journal审稿人（keywords:AFM, DNA molecules, Nicks）
请站内联系并附上 姓名，邮箱，工作单位，研究方向，两篇相关代表作。 |
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q**********0
发帖数: 335 | 27 There are two RNAs, one is about 1800bp, one is 200bp, how to know these two
RNA molecule will complementary to each other? What kind of software we can
use? Thanks. |
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x*****o
发帖数: 441 | 28 这个老板自己是single molecule的吗?没人带白手起家可不容易.从实验到data
analysis都会有问题的. |
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x*****o
发帖数: 441 | 29 Ha其实传统single molecule技术这方面水平还行,review什么的写得也很不错. |
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m******h
发帖数: 32 | 30 曾经设计过一个课题想用single molecule的技术，半年后让老板枪毙掉了。不是他的
兴趣所在，不愿意再花更多的人力物力了。 |
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s*****j
发帖数: 6435 | 31 "sunny xie的活细胞confocal", 这个是什么东西?
"小薇他们在做的super resolution", super resolution 也算成是single molecule? |
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x*****o
发帖数: 441 | 32 就是用confocal显微镜看单个细胞里面的荧光,主要是FRET
molecule? |
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b******y
发帖数: 627 | 33 I am not 业内人士. But I am a big fan of single molecule and high resolution
imaging techniques. So are many decent biophysics, biochemistry and cell
biology departments.
For the next few decades, "seeing is believing" is still the rule of thumb
in biology. By that, I mean there are a lot of applications of these
imagining techniques to otherwise intractable biological questions. Be an
expert of it technically and be able to ask the right biological questions (
as pointed out by pll) and you will fi... 阅读全帖 |
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p*l
发帖数: 1359 | 34 single molecule和high resolution imaging要做到物理工程加上生物紧密结合，明了各个环节的机会和局限性，这一行才能做下去。生物背景的想跳这一行的，要问问自己，如果要读生物PhD的同时做一个EE或者Physics PhD，有没有这个能力。
resolution
( |
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x*****o
发帖数: 441 | 35 好像前几年发个一片science,拉曼光谱的,2008吧.
最近拉曼光谱也挺火的,前几年还听一个seminar,是一个是IR的做single molecule的..
...... |
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p*l
发帖数: 1359 | 36 这些新方法，还远没达到能让一个做生物的人打开开关就用的地步，对技术不了解，不
可能做好。而且各个方法也有不同的应用局限性，能不能选到一个有意义的应用，往往
决定了一个新技术的命运。在生物应用上，往往需要根据仪器的局限调整生物方面的实
验方案。在图像数据处理上，也需要根据情况调整处理算法。所以，在single
molecule和super resolution领域，只有做到造显微镜，用显微镜以及分析解释数据一
体，才能有好结果。
这个方向现在百花齐放，机会很多，恰恰是因为并没有一个压倒一切，而且能让一般生
物研究人员就能使用的成熟技术。 |
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v*****s
发帖数: 20290 | 37 我比较奇怪的是，怎么shuming nie一直没人提呢？这个也很牛的啊。97年那片single
molecule SERS现在引用快4000了吧。 |
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s******9
发帖数: 283 | 38 统计问题纠结了好久，想咨询一下：
以前做biochemistry，用纯化样品，比如酶，triplicate结果就可以发表了，大家一般
就做个standard deviation的error bar。
现在类似的实验，检测single-molecule信号。比如挑5000个分子，然后算出有信号的
比例（不好简单地估计standard deviation）。我现在的操作也是也triplicate，然后
算出error bar。
我的问题是，有没有必要算confidence interval？比如用Student’s t test，n=3，
confidence interval用实验的standard deviation算，感觉就像是个redundant的值。
不知道这种基于单分子平均信号的数据，有没有标准的处理？ |
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Y********6
发帖数: 51 | 39 We will provide the service package such as trainning people, assemble the
instrument and provide guidance for experimental design. Single molecule
FRET does not have patent issue and the patent of spinning disc is going to
expire soon. We are aware of this issue, so we will do whatever we can do. |
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m********r
发帖数: 124 | 40 有没有人给推荐用single molecule fluorescence做蛋白dynamics conformation的.
怎么放眼望去都是做DNA的各种酶的？有没有做蛋白构象或者相互作用的？ |
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c*******0
发帖数: 190 | 41 Single molecule FRET. Try it. |
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h*******1
发帖数: 13 | 42 Single Molecules Meet Genomics
Pinpointing Precision Medicine
http://jama.jamanetwork.com/article.aspx?articleid=2293457&utm_
粗粗看了一遍， 不是很了解这方面的研究。有几个问题请教专家：
关于血液中的肿瘤单细胞测序：如果血液中的肿瘤细胞超过两个并且这些肿瘤细胞之间
也存在基因序列上的不同，如何进行肿瘤鉴定和分析。
关于体外受精的临床试验：从发育的第五天到第六天获取细胞样品进行测序的伦理学问题
关于标题：单细胞测序到底如何使得个人化医学受益，在这方面没有看明白作者的解释 |
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a****k
发帖数: 117 | 43 需要三个journal审稿人（keywords:AFM, DNA molecules, Nicks）
请站内联系并附上 姓名，邮箱，工作单位，研究方向，两篇相关代表作。 |
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w**k
发帖数: 6722 | 44 LC-MS/MS貌似只能定量不能知道具体的number of protein molecules（用浓度来推算
当然可以，不过不算digital方法吧） |
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c********6
发帖数: 658 | 45 how many molecule are in a 100mg sample of water?
how many atoms of hydrogen are in a 100mg sample of wa |
|
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l*l
发帖数: 149 | 47 Richard F. Heck, Ei-ichi Negishi and Akira Suzuki share the 2010
Nobel Prize in Chemistry for developing new, more efficient ways of linking
carbon atoms together to build the complex molecules that are improving our
everyday lives. |
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a***n
发帖数: 578 | 48 【 以下文字转载自 Chemistry 讨论区,原文如下 】
发信人: argon (argon), 信区: Chemistry
标 题: Question: how to synthesis this molecule?
发信站: The unknown SPACE (Mon Jan 20 18:12:44 2003) WWW-POST
3 fluoro - imidazole? None seems to have done that.
F3C3N2H
any organic buddy will give a hint? thanks a lot. |
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a****k
发帖数: 117 | 49 需要三个journal审稿人（keywords:AFM, DNA molecules, Nicks）
请站内联系并附上 姓名，邮箱，工作单位，研究方向，两篇相关代表作。 |
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