v*******n
发帖数: 8995 | 1 这个
是molecualr beacon
或者taqman
正反是用来pcr
最后的那段是反向的探针在目标rna上
3‘的把5’的荧光抑制 不发光
等pcr开始了 到了那段把丫分解 或打算就尼玛发光了
这个比较准确 假阳性不多
★ 发自iPhone App: ChinaWeb 1.1.5 |
|
v*******n
发帖数: 8995 | 2 这个
是molecualr beacon
或者taqman
正反是用来pcr
最后的那段是反向的探针在目标rna上
3‘的把5’的荧光抑制 不发光
等pcr开始了 到了那段把丫分解 或打算就尼玛发光了
这个比较准确 假阳性不多
★ 发自iPhone App: ChinaWeb 1.1.5 |
|
a***k
发帖数: 1038 | 3 你说的原理我这个外行看下维基早就明白了。我闹不懂的是金银潭医院的用的引物找不
到对应的序列。
至于选择性，不是说新病毒和舟山的蝙蝠的病毒相似99以上，难道随便切的一个片段不
可以和新病毒一样吗？
: 这个
: 是molecualr beacon
: 或者taqman
: 正反是用来pcr
: 最后的那段是反向的探针在目标rna上
: 3‘的把5’的荧光抑制 不发光
: 等pcr开始了 到了那段把丫分解 或打算就尼玛发光了
: 这个比较准确 假阳性不多
: ★ 发自iPhone App: ChinaWeb 1.1.5
|
|
L******n
发帖数: 20 | 4 Thanks for all your replies.
To: Bigbigbee
I mainly focused on growth and characterization of thin films by Molecualr
beam epitaxy (MBE). The thin films have almost no applications and I have to
rely on MBE. For MBE related positions, they require at least green card.
If someone could recommend, I could have a change in bigger companies which
need general skills in our field.
If any of you could recommend me, I will really appreciate that (:.
Best wishes to EVERYONE. |
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g***m
发帖数: 465 | 5 check NEB catalog for Taq enzyme, it has double strand specific 5'->3'
exonuclease activity.
My guess is primer and/or product degradation by Taq.
BTW, one small tip for pfx is: using 2X buffer rather than 1X. I got several
cDNA clones by PCR, and none of them disrupt the ORF by this modification.
DNA(WASP)
然后用同样的primer
colony-pGEX4t1
这些在处理后的pGEX4t1 |
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w***a
发帖数: 4361 | 6 问题是做了这么多年，很多疾病相关的signaling pathway已经逐渐明了。target就在那
里，问题是不drugable。
RNAi的好处太多了，不是单个基因控制的diease，你就多弄几条siRNA target多个基因
，甚至整条pathway。这个用small molecular是不可想象的。要说off target effect，
small molecular就更没法控制了。至于RNAi的作用是short term而非long term，这点
跟small molecualr也一样，而且实际上你也不希望吃点药就permanently knock out你的
某个基因吧？
对于RNAi therapy，其实就是个delivery的问题。 |
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c********r
发帖数: 1125 | 7 我不太清楚童的工作，因为查不到原始paper.很怀疑有没有人能重复出来实验。
也不用过分夸大童的意义，因为胚胎学很大程度上是技术活，那个年代也是molecualr
biology得年代，胚胎学（包括gurdon ）也被遗忘了很久。
没有yamanaka的工作，gurdon的工作也未必100％就得奖。
当然中国也有很牛的工作，比如前几天michael bishop在我们这里的talk化了30分钟的
时间回顾了中国王振义＋张廷栋的 全反维甲酸＋As2O3治疗APL白血病的工作，给了很
高的评价。虽然有狗屎运成分，但是这个研究我们是世界最前列得，也是目前为止药物
能几乎治愈的唯一肿瘤。。。 |
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p****a
发帖数: 42 | 8 Postdoc Positions (individuals with high qualifications can be appointed as
research scientists; Ph.D. studentship is also available).
Location: Houston, Texas
Excellent programming skill is a must.
Preferred: database construction and mining, and web-based application and user
interface development
Desired:
Knowledge of drug discovery, QSAR, ADMET, molecualr docking, machine learning, or
cheminformatics/bioinformatics/systems biology
We have VERY good benefit packages, office environment, and r |
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p****a
发帖数: 42 | 9 Postdoc Positions (individuals with high qualifications can be appointed as
research scientist; Ph.D. studentship is also available).
Location: Houston, Texas
Excellent programming skill is a must.
Preferred: database construction and mining, and web-based application and user
interface development
Desired:
Knowledge of drug design, QSAR, ADMET, molecualr docking, machine learning,
or cheminformatics/bioinformatics/systems biology
We have VERY good benefit packages, office environment, and resea |
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t*******n
发帖数: 66 | 10 In my opinion, generally speaking, PVA can be dissoved in water. But when the
molecualr is too high(> 10,000), it will be unsoluble in water, maybe because
of the hrdrogen bond. So probably you can make some more short block of
PVA(probably 80 or 100 DPn). I thin it wil work.
PIB |
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j*********e
发帖数: 1 | 11 Hi, I got the PhD in biochemistry and molecualr biology in 2005 followed by
2 years postdoc training in proteomic project in university of california
I want to apply a job in biophamaceutical company at scientist level in US
or in China.
Does anyone have some informaiton about this?
thanks. |
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p****a
发帖数: 42 | 12 Postdoc Positions (individuals with high qualifications can be appointed as
research scientists; Ph.D. studentship is also available).
Location: Houston, Texas
Excellent programming skill is a must (not necessary for studentship applicants).
Preferred: database construction and mining, and web-based application and user
interface design
Desired:
Knowledge of drug design, QSAR, ADMET, molecualr docking, machine learning, or
cheminformatics/bioinformatics/systems biology.
We have VERY good benefit |
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a***u
发帖数: 72 | 13 哦, Beer's Law, 也叫Lamb-Beer's Law.对于理想吸光.
A=Ln[I_{Transmission}/I_{Incident}]=\epsilon c L,
c是吸光组分的浓度,L是吸收光程,\epsilon叫absorbtivity(比吸光度).基于Lamb-Beer's
Law的成分分析也叫 UV/Vis molecualr absorption analysis, 不过精度不高,除非对高
吸光度的成分.但对天然色素,如卟啉类大环色素(P P*吸收带)比吸光度可高达10^4至10^5
L {mol}^{-1} {cm}^{-1}的检出限还是很底的.
不过因为UV/Vis都是带状谱,灵敏度,抗干扰度,没法和AAS,或ICP-AES比啦.Lamb-Beer's
Law的线性区间也不是很大的说.
？
况
.
吸
如
没辙。。。我也看不懂TA在说什么。对了，有点儿事儿跟你商量一下，给我发个MSG |
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