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e*****r
发帖数: 379 | 2 One poss reason: MeCN too high!
try 90 or 85% MeCN
with some ammonium acetate. |
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S********8
发帖数: 781 | 4 If I were you, I will disassamble all pumps, tubes and parts, then replace
them with new tubes, new parts, leave pumps there if they works well.
Next step after re assambling, manually calibrate the flow rate with water,
MeCN or MeOH, with or without C18 column, and verify the pressure
Check the UV detector to see if the light bubble still works well or replace
it with new light bubble
The reparing costs are very expensive and may allow you to buy a new HPLC
system if you ask experts to do it, S... 阅读全帖 |
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h******g
发帖数: 600 | 5 诸如 [Cu(MeCN)4]BF4 在酰氯-COCl存在下稳定吗，会有什么副反应吗？谢谢了。 |
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l*******e
发帖数: 1519 | 6 生成的MoOL配合物易溶于MeCN，CH2Cl2。但是不确定在hexane 和乙醚的溶解性。所以
问一下你这个冲洗剂怎么选择 的。（Ph3PO)易溶于极性溶剂。最初 我试了一下，用的
二氯甲烷和甲醇，但是冲不下来。 |
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m******3
发帖数: 108 | 9 最近在固相合成易形成beta-sheet 的一段较长的peptide，其中侧链中酰胺键较多，在
含有TFA的
MeCN/H2O体系中过完HPLC后，冻干前用纯水怎么都没法完全溶解。
不晓得版上有没有做过类似东东的朋友，有啥建议。 |
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m******3
发帖数: 108 | 10 抱歉，没表述清楚，HPLC的后的溶液是MeCN/H20/TFA，然后旋蒸掉溶剂后，加纯水希望
可以溶解后去
冻干，这步溶解很困难。 |
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w*s
发帖数: 50 | 11 Prep HPLC，样品不溶于水，在乙腈里溶解度极低。只溶于DCM或DMSO，怎样上样啊？起
始洗脱50% MeCN/H2O。老问题了，一直找不到好的solution。只有一根柱子，不敢用太
多DMSO上啊。 |
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n*******9
发帖数: 277 | 12 http://www.bestnursingdegree.com/programs/direct-entry-msn/
Show me all the Direct Entry MSN programs in:
Alabama
The University of Alabama at Birmingham (Birmingham, AL)
Online Program Name: Accelerated Masters in Nursing Pathway (AMNP) - CNL
Accreditation: CCNE accredited
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Online Program Name: Clinical Nurse Leader, Executive Nurse Administrator,
Nursing Education
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Arizona
University of Arizona (Tucson, AZ)
Online Program Na... 阅读全帖 |
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n*******9
发帖数: 277 | 13 http://www.bestnursingdegree.com/programs/direct-entry-msn/
Show me all the Direct Entry MSN programs in:
Alabama
The University of Alabama at Birmingham (Birmingham, AL)
Online Program Name: Accelerated Masters in Nursing Pathway (AMNP) - CNL
Accreditation: CCNE accredited
University of South Alabama (Mobile, AL)
Online Program Name: Clinical Nurse Leader, Executive Nurse Administrator,
Nursing Education
Accreditation: CCNE accredited
Arizona
University of Arizona (Tucson, AZ)
Online Program Na... 阅读全帖 |
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