j*b
发帖数: 341 | 1 这个PKM2和肿瘤代谢的关系已经知道很久了，这两篇还不是原创贴。
cantley还可以发nature，能不能解释一下？
Anticancer Res. 2003 Mar-Apr;23(2A):1155-8.
Tumor marker pyruvate kinase type tumor M2 in patients suffering from
diabetic nephropathy.
Oremek GM, Rutner F, Sapoutzis N, Sauer-Eppel H.
Source
Central Laboratory, Hospital of the Johann Wolfgang Goethe-University,
Frankfurt/Main, Germany.
Abstract
INTRODUCTION:
Compared to normal tissues all tumors investigated so far and especially
those with a metastatic potential overexpress an isoenzy... 阅读全帖 |
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j*b
发帖数: 341 | 2 这个PKM2和肿瘤代谢的关系已经知道很久了，这两篇还不是原创贴。
cantley还可以发nature，能不能解释一下？
Anticancer Res. 2003 Mar-Apr;23(2A):1155-8.
Tumor marker pyruvate kinase type tumor M2 in patients suffering from
diabetic nephropathy.
Oremek GM, Rutner F, Sapoutzis N, Sauer-Eppel H.
Source
Central Laboratory, Hospital of the Johann Wolfgang Goethe-University,
Frankfurt/Main, Germany.
Abstract
INTRODUCTION:
Compared to normal tissues all tumors investigated so far and especially
those with a metastatic potential overexpress an isoenzy... 阅读全帖 |
|
G***a
发帖数: 27294 | 3 根据他给的wiki
http://en.wikipedia.org/wiki/Skeletal_muscle
“ The fast twitch fibers rely on a well-developed, short term, glycolytic
system for energy transfer and can contract and develop tension at 2-3 times
the rate of slow twitch fibers.”
---glycolytic system是糖酵解酶系统。这段话意思难道不是说fast twitch是无氧能
力强，非常快产能的意思吗？
-----------------------
看到后面，又发现，红肌是slow twitch， 两种白肌都叫fast twitch
要么是wiki后面修订错了，要么是书上有错。现在没法确定，我要travel，回到家再仔
细看paper吧：） |
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a******1
发帖数: 1519 | 4 Skeletal (voluntary) muscle is further divided into two broad types: slow
twitch and fast twitch:
Type I, slow twitch, or "red" muscle, is dense with capillaries and is rich
in mitochondria and myoglobin, giving the muscle tissue its characteristic
red color. It can carry more oxygen and sustain aerobic activity using fats
or carbohydrates as fuel.[4] Slow twitch fibers contract for long periods of
time but with little force.
Type II, fast twitch muscle, has three major subtypes (IIa, IIx, and I... 阅读全帖 |
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b****u
发帖数: 903 | 6 Acta Crystallogr D Biol Crystallogr. 2008 Jun;64(Pt 6):651-7. Epub 2008 May
14.
Structure of human alpha-enolase (hENO1), a multifunctional glycolytic
enzyme.
many thanks.
please send to: a******[email protected] |
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n***w
发帖数: 2405 | 7 Proteomic profiling of a layered tissue reveals unique glycolytic
specializations of photoreceptor cells.
Reidel B, Thompson JW, Farsiu S, Moseley MA, Skiba NP, Arshavsky VY.
PMID: 21173383
我们学校明明买了这个杂志的，不知道为什么获取全文时还要sign in。。。
请发到n***[email protected]
非常感谢！ |
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h*******o
发帖数: 4884 | 8 As mentioned above, a lot of the cytotoxicity/cell viability assays
are based on mitochondrial function, particularly SDH activity. This renders
a lot of the assays under question when the "manipulation/treatment" have
potential impacts on mitochondrial function.
For cancer cell lines, it is less of an issue because of the
notorious "warburg" effect through which cancer cells are more glycolytic
and rely much less on mitochondrial OXPHOS.
But for primary cultures, like... 阅读全帖 |
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h******y
发帖数: 351 | 9 Actually, tumor cells do not prefer to use oxygen. The so-called Warburg
effect is the observation that most cancer cells predominantly produce
energy by a high rate of glycolysis followed by lactic acid fermentation in
the cytosol, rather than by a comparatively low rate of glycolysis followed
by oxidation of pyruvate in mitochondria like most normal cells.
Unlike what we would think, malignant rapidly-growing tumor cells typically
have glycolytic rates that are up to 200 times higher than thos... 阅读全帖 |
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l**********1
发帖数: 5204 | 10 RT
by
Postnikov EB, Verveyko DV, Verisokin AY
Simple model for temperature control of glycolytic oscillations.
Phys Rev E Stat Nonlin Soft Matter Phys. 2011 Jun;83(6 Pt 1):062901. Epub
2011 Jun 16.
ncbi link:
http://www.ncbi.nlm.nih.gov/pubmed/21797427
my mail address:
Thanks a million in advance! |
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j********r
发帖数: 156 | 11 碰巧刚看到几篇关于氨基酸代谢的文章，大牛来点评一下。
1. Serine starvation induces stress and p53-dependent metabolic remodelling
in cancer cells （nature, 2013)
2. Serine, but Not Glycine, Supports One-Carbon Metabolism and Proliferation
of Cancer Cells (Cell reports, 2014)
3. Phosphoglycerate dehydrogenase diverts glycolytic flux and contributes to
oncogenesis (Nature genetics, 2011)
4. Functional genomics reveal that the serine synthesis pathway is essential
in breast cancer (nature, 2011)
elife刚发了一片关于metformin的文章。俺愣是没看... 阅读全帖 |
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p*****h
发帖数: 36 | 12 Suicide inhibitor is a term reserved for those capable of covalent
modification of enzyme active site. Of course at high concentrations, 2DG6P
as a substrate /product analogue inhibits several glycolytic enzymes
including HK and PFK.
It is for historical reasons that these drugs are dirty (phenotypic drug
screening came well before rational design). This is not to say there will
be no better drugs.
在 hellozero (hellozero) 的大作中提到: 】
s
inhibitors/ |
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h*******o
发帖数: 4884 | 13 pigfish was right, 2-DG usually is not called as hexokinase inhibitor but
glycolytic inhibitor.
There are many other metabolic modulators that you can use, but keep in mind
, they often come with primary and secondary impact of metabolic flux and
you should tightly control your study so that the outcome can be interpreted
properly. |
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u**s
发帖数: 384 | 14 Title:A method for the determination of changes of glycolytic metabolites in
yeast on a subsecond time scale using extraction at neutral pH
Author:de Koning W, van Dam K.
Journal:Anal Biochem. 1992 Jul;204(1):118-23.
Link:http://www.ncbi.nlm.nih.gov/pubmed/1514678
3x! |
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s*******d
发帖数: 1079 | 15 学友MILK找到的这个FA ２０１１版 vs ２０１０版的详细对照，我觉得很有帮助，放
在这儿给大家做参考吧。
原文见 http://forums.studentdoctor.net/showthread.php?t=785943
Overall:
- FA 2011 corrects some of the 2010 errata
- FA 2011 has darker print and thicker pages
- FA 2011 increases the size of most figures/images (although only slightly)
- FA 2011 removes several images that are difficult to see/interpret
Behavioral Science:
p. 59-60 2010 (57-58 2011) -2011 reorganized a couple items into "Advance
directives" and got rid of "Good Samaritan Law.... 阅读全帖 |
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h*******o
发帖数: 4884 | 16 That is not necessarily the case.
For metabolic coupling, glia are largely glycolytic relative to neurons. Yet
, they provide substrates for OXPHOS in neurons. Glia covert glucose to
pyruvate, then to lactate, which is transported to neurons and converted
back to pyruvate to enter into the TCA cycle and OXPHOS.
For more details, read Pierre Magistratti's review on glia-neuron metabolic
corss talk reviews.
For anti-oxidant capacity, glia are far more resistant to nitric oxide and
other ROS. They ... 阅读全帖 |
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